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Fluorescent quenching-based quantitative detection of specific DNA/RNA using a BODIPY(R) FL-labeled probe or primer
Open accessAbstract
We have developed a simple method for the quantitative detection of specific DNA or RNA molecules based on the finding that BODIPY((R)) FL fluorescence was quenched by its interaction with a uniquely positioned guanine. This approach makes use of an oligonucleotide probe or primer containing a BODIPY((R)) FL-modified cytosine at its 5'-end. When such a probe was hybridized with a target DNA, its fluorescence was quenched by the guanine in the target, complementary to the modified cytosine, and the quench rate was proportional to the amount of target DNA. This widely applicable technique will be used directly with larger samples or in conjunction with the polymerase chain reaction to quantify small DNA samples.
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Cites
Primer-Directed Enzymatic Amplification of DNA with a Thermostable DNA Polymerase
Saiki, R. K., Gelfand, D. H., Stoffel, S., Scharf, S. J., Higuchi, R., Horn, G. T., Mullis, K. B., & Erlich, H. A. (1988) · Science · doi:10.1126/science.2448875 · Cited by 17,719
Cited by
BODIPY Dyes and Their Derivatives: Syntheses and Spectroscopic Properties
Loudet, A., & Burgess, K. (2007) · Chemical Reviews · doi:10.1021/cr078381n · Cited by 5,238
Bias and artifacts in multitemplate polymerase chain reactions (PCR)
Kanagawa, T. (2003) · Journal of Bioscience and Bioengineering · doi:10.1016/s1389-1723(03)90130-7 · Cited by 566
Real‐Time Polymerase Chain Reaction
Wilhelm, J., & Pingoud, A. (2003) · ChemBioChem · doi:10.1002/cbic.200300662 · Cited by 396
Real-Time Multiplex PCR Assays
Wittwer, C. T., Herrmann, M. G., Gundry, C. N., & Elenitoba-Johnson, K. S. J. (2001) · Methods · doi:10.1006/meth.2001.1265 · Cited by 314
Detection and Enumeration of Aromatic Oxygenase Genes by Multiplex and Real-Time PCR
Baldwin, B. R., Nakatsu, C. H., & Nies, L. (2003) · Applied and Environmental Microbiology · doi:10.1128/aem.69.6.3350-3358.2003 · Cited by 290
Metadata
- Authors
- Kurata, S.
- Venue
- Nucleic Acids Research
- Publisher
- Oxford University Press (OUP)
- Published
- 2001-03-15
- Volume
- 29
- Issue
- 6
- Pages
- 34e-34
- ISSN
- 0305-1048 (ISSN-L)
- 1362-4962
- OpenAlex
- W1991525069
- Cited by
- 198
- References
- 1
- FWCI
- 2.82
- Type
- journal article
- Language
- en
- Open access
- green
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- Kurata, S. (2001). Fluorescent quenching-based quantitative detection of specific DNA/RNA using a BODIPY(R) FL-labeled probe or primer. Nucleic Acids Research. https://doi.org/10.1093/nar/29.6.e34
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