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A method for the recovery of DNA from agarose gels

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Tabak, H.F., Flavell, R.A.·1978·Nucleic Acids Research

Abstract

We describe a quick and versatile method for the isolation of DNA from agarose gels. The DNA is electrophoresed into a trough containing hydroxyapatite, where it is bound. The hydroxyapatite is taken out and the DNA eluted with phosphate buffer. By putting the hydroxyapatite on a small column of Sephadex G50, elution and subsequent removal of phosphate can be performed in one step. The DNA recovered can be used equally well in enzymatic incubations as DNA not purified through agarose gel electrophoresis. Several applications of this technique are described.

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Metadata

Authors
Tabak, H. F., & Flavell, R. A.
Venue
Nucleic Acids Research
Published
1978
Volume
5
Issue
7
Pages
2321-2331
ISSN
  • 0305-1048 (ISSN-L)
  • 1362-4962
OpenAlex
W2024662909
Cited by
261
References
0
FWCI
20.25
Type
journal article
Language
en
Open access
green

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CoChat. (2026). Citation check: A method for the recovery of DNA from agarose gels. https://app.cochat.ai/papers/10.1093/nar/5.7.2321
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Tabak, H. F., & Flavell, R. A. (1978). A method for the recovery of DNA from agarose gels. Nucleic Acids Research. https://doi.org/10.1093/nar/5.7.2321

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