Nuclear RNA transcripts from drosophila melanogaster ribosomal RNA genes containing introns
Open accessAbstract
The transcription of ribosomal genes in a cell line (Kc) derived from female D.melanogaster, has been investigated using hybridization probes prepared from restriction fragments of a cloned rDNA repeat with a 5 kb type I [Wellauer et al. (1978) Cell 14, 269-278] intron. Gels, of nuclear RNA that have been transferred to diazotized paper and hybridized with labelled intron sequences, reveal both large (1-10 kb) transcripts and a discrete 325 base species. Berk-Sharp experiments [(1977) Cell 12, 721-732] reveal large transcripts that are homologous to intron sequences and extend into 28S sequences as well. However, while the abundance of 28S transcripts is about 50,000 copies per nucleus [Clark et al. (1977) Genetics 86, 789-800], these long transcripts are only present at 1-2 copies per nucleus and the 325 base species is only 10 times more abundant. In view of the fact that female cells have about 400 rDNA genes, 49% of which have type I introns, one must conclude either that transcription rarely occurs from the genes containing introns (the majority) or these transcripts are processed unusually rapidly. Transcripts homologous to the "non-transcribed spacer" region have been found, but their abundance is no higher.
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rRNA genes with insertions produce hundreds or thousands of times fewer transcripts than intact rDNAs, though one work observed only a 10-fold difference.
“In fact, it has been known for several decades that rRNA genes with insertions (hereafter, inserted or interrupted rDNAs) produce hundreds or thousands of times fewer transcripts than intact (uninserted) rDNAs (47–49), though in one work only a 10-fold difference was observed (50).”
From Impaired function of rDNA transcription initiation machinery leads to derepression of ribosomal genes with insertions of R2 retrotransposon (2022) · cites this paper as [48]
The Results and Discussion report that intron sequences were about 3,000 times less abundant than exon sequences in nuclear RNA from D. melanogaster Kc cells. But the paper says the low abundance could reflect either rare transcription or rapid breakdown; it does not establish that inserted genes produce transcripts 3,000 times less often. The 10-fold observation is attached to a different citation in the citing sentence. (LLM, conf=0.86)
Experimental — accuracy not yet measuredA substantial number of rDNA units in the D. melanogaster genome contain insertions of R1 and R2 retrotransposons usually causing these units to be expressed at very low levels.
“It has been known for several decades that a substantial number of rDNA units in the D. melanogaster genome contain insertions of R1 and R2 retrotransposons usually causing these units to be expressed at very low levels (47,48,51).”
From Impaired function of rDNA transcription initiation machinery leads to derepression of ribosomal genes with insertions of R2 retrotransposon (2022) · cites this paper as [48]
The Introduction (page unavailable) says that about 49% of rDNA repeats in the female-derived cells studied have type I introns. The Abstract (page unavailable) reports very low abundance of long intron-containing transcripts, but does not establish that the insertions cause low expression; it also raises rapid processing as a possibility. Our copy does not identify the introns as R1 and R2 retrotransposons. (LLM, conf=0.86)
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Research works printed but not linked: 7
- [6]6. Tilghman, S.M., Tiemeier, D.C., Seidman, J.G., Peterlin, B.M., Sullivan, M., Maizel, J.V. and Leder, P. (1978) Proc. Natl. Acad. Sci. USA 75, 725-729.
- [19]19. 19, Dawid, I.B. and Botchan, P. (1977) Proc. Natl. Acad. Sci. USA 74, 4233-4237.
- [25]25. Alwine, J.C., Kemp, D.J. and Stark, G.R. (1977) Proc. Natl. Acad. Sci. USA 74,5350-5354.
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- [31]31. Clewe11, D.B. and Helinski, D.R. (1969) Proc. Natl. Acad. Sci.USA 62, 1159-1166.
- [35]35. Maniatis, T., Jeffrey, A. and Kleid, D.G. (1975) Proc. Nat1, Acad. Sci. USA 72, 1184-1188.
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Cited by
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Jamrich, M., & Miller, O. L. (1984) · The EMBO Journal · doi:10.1002/j.1460-2075.1984.tb02008.x · Cited by 66 · see citing works
Repression of interrupted and intact rDNA by the SUMO pathway in drosophila melanogaster
Luo, Y., Fefelova, E., Ninova, M., Chen, Y.-C. A., & Aravin, A. A. (2020) · eLife · doi:10.7554/elife.52416 · Cited by 19 · see citing works
Fefelova, E. A., Pleshakova, I. M., Mikhaleva, E. A., Pirogov, S. A., Poltorachenko, V. A., Abramov, Y. A., Romashin, D. D., Shatskikh, A. S., Blokh, R. S., Gvozdev, V. A., et al. (2022) · Nucleic Acids Research · doi:10.1093/nar/gkab1276 · Cited by 15 · see citing works
“melanogaster genome contain insertions of R1 and R2 retrotransposons usually causing these units to be expressed at very low levels (47,48,51).”
Background
- Authors
- Jolly, D. J., & Thomas, C. A.
- Affiliations
- Scripps Clinic (US)
- ORCID
- Jolly, D.J.
- Venue
- Nucleic Acids Research
- Publisher
- Oxford University Press (OUP)
- Published
- 1980
- Volume
- 8
- Issue
- 1
- Pages
- 67-84
- ISSN
- 0305-1048 (ISSN-L)
- 1362-4962
- PMC
- PMC327243
- OpenAlex
- W2046131886
- Cited by
- 43
- References
- 0
- Citations by year
- 2021: 1 · 2020: 1 · 2013: 2
- FWCI
- 4.21
- Type
- journal article
- Language
- en
- Open access
- green
- europepmc.org
- Locations
- Nucleic Acids Research (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Charles A. King Trust
- University of New South Wales
- University of California, San Diego
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Nuclear Structure and Function
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Chromosomal and Genetic Variations
- Keywords
- ribosomal RNA gene transcription, Drosophila melanogaster, 28S rRNA, non-transcribed spacer, rDNA repeats, mRNA levels, RNA processing
- MeSH terms
- Animals, Cell Line, Cell Nucleus, Cloning, Molecular, DNA Restriction Enzymes, DNA, Recombinant, Drosophila melanogaster, Female, Molecular Weight, Nucleic Acid Hybridization, Plasmids, RNA, RNA, Ribosomal, Transcription, Genetic
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