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Phy M: an RNase activity specific for U and A residues useful in RNA sequence analysis
Open accessAbstract
Physarum polycephalum, an acellular slime mold, produces an endoribonuclease activity (Phy M) useful in direct RNA sequence determination. Under conditions previously described for direct enzymatic RNA sequencing (7M urea, 50 degrees C, pH 5.0)1 Phy M cleaves almost exclusively and uniformly at Up-N and Ap-N. In the absence of urea Up-N, Ap-N, and Gp-N are attacked in a sequence specific way identical to that found with RNase Phy I2 digests performed under the same reaction conditions.
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Cited by
A General Method for Site-specific Incorporation of Unnatural Amino Acids into Proteins
Noren, C. J., Anthony-Cahill, S. J., Griffith, M. C., & Schultz, P. G. (1989) · Science · doi:10.1126/science.2649980 · Cited by 1,019
A new method of sequencing DNA
Hyman, E. D. (1988) · Analytical Biochemistry · doi:10.1016/0003-2697(88)90041-3 · Cited by 272
Yasukawa, T., Suzuki, T., Suzuki, T., Ueda, T., Ohta, S., & Watanabe, K. (2000) · Journal of Biological Chemistry · doi:10.1074/jbc.275.6.4251 · Cited by 268
Yasukawa, T., Suzuki, T., Ishii, N., Ueda, T., Ohta, S., & Watanabe, K. (2000) · FEBS Letters · doi:10.1016/s0014-5793(00)01145-5 · Cited by 124
Lipskaya, G. Y., Muzychenko, A. R., Kutitova, O. K., Maslova, S. V., Equestre, M., Drozdov, S. G., Bercoff, R. P., & Agol, V. I. (1991) · Journal of Medical Virology · doi:10.1002/jmv.1890350415 · Cited by 97
Metadata
- Authors
- Donis-Keller, H.
- Venue
- Nucleic Acids Research
- Publisher
- Oxford University Press (OUP)
- Published
- 1980
- Volume
- 8
- Issue
- 14
- Pages
- 3133-3142
- ISSN
- 0305-1048 (ISSN-L)
- 1362-4962
- OpenAlex
- W2083003129
- Cited by
- 321
- References
- 0
- FWCI
- 12.02
- Type
- journal article
- Language
- en
- Open access
- bronze
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- Donis-Keller, H. (1980). Phy M: an RNase activity specific for U and A residues useful in RNA sequence analysis. Nucleic Acids Research. https://doi.org/10.1093/nar/8.14.3133
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