The DOI matches its Crossref record; 22 of the 22 references it cites were found in a registry or an index.
Shotgun DNA sequencing using cloned DNase I-generated fragments
Open accessAbstract
A method for DNA sequencing has been developed that utilises libraries of cloned randomly-fragmented DNA. The DNA to be sequenced is first subjected to limit attach by a non-specific endonuclease (DNase I in the presence of Mn++), fractionated by size and cloned in a single-stranded phage vector. Clones are then picked at random and used to provide a template for sequencing by the dideoxynucleotide chain termination method. This technique was used to sequence completely a 4257 bp EcoRI fragment of bovine mitochondrial DNA. The cloned fragments were evenly distributed with respect to the EcoRI fragment, and completion of the entire sequence required the construction of only a single library. In general, once a clone library has been prepared, the speed of this approach (greater than 1000 nucleotides of randomly selected sequence per day) is limited mainly by the rate at which the data can be processed. Because the clones are selected randomly, however, the average amount of new sequence information per clone is substantially diminished as the sequence near completion.
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Citations
Cites
DNA sequencing with chain-terminating inhibitors
Sanger, F., Nicklen, S., & Coulson, A. R. (1977) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.74.12.5463 · Cited by 68,572
Rigby, P. W. J., Dieckmann, M., Rhodes, C., & Berg, P. (1977) · Journal of Molecular Biology · doi:10.1016/0022-2836(77)90052-3 · Cited by 11,401
Sequence and organization of the human mitochondrial genome
Anderson, S., Bankier, A. T., Barrell, B. G., de Bruijn, M. H. L., Coulson, A. R., Drouin, J., Eperon, I. C., Nierlich, D. P., Roe, B. A., Sanger, F., et al. (1981) · Nature · doi:10.1038/290457a0 · Cited by 9,636
Self-citationCloning in single-stranded bacteriophage as an aid to rapid DNA sequencing
Sanger, F., Coulson, A. R., Barrell, B. G., Smith, A. J. H., & Roe, B. A. (1980) · Journal of Molecular Biology · doi:10.1016/0022-2836(80)90196-5 · Cited by 3,407
A system for shotgun DNA sequencing
Messing, J., Crea, R., & Seeburg, P. H. (1981) · Nucleic Acids Research · doi:10.1093/nar/9.2.309 · Cited by 2,619
The isolation of structural genes from libraries of eucaryotic DNA
Maniatis, T., Hardison, R. C., Lacy, E., Lauer, J., O'Connell, C., Quon, D., Sim, G. K., & Efstratiadis, A. (1978) · Cell · doi:10.1016/0092-8674(78)90036-3 · Cited by 1,825
Yamamoto, K. R., Alberts, B. M., Benzinger, R., Lawhorne, L., & Treiber, G. (1970) · Virology · doi:10.1016/0042-6822(70)90218-7 · Cited by 1,565
The use of thin acrylamide gels for DNA sequencing
(1978) · FEBS Letters · doi:10.1016/0014-5793(78)80145-8 · Cited by 1,433
Sutcliffe, J. G. (1978) · Nucleic Acids Research · doi:10.1093/nar/5.8.2721 · Cited by 759
Messing, J. W., Gronenborn, B., Müller‐Hill, B., & Hopschneider, P. H. (1977) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.74.9.3642 · Cited by 705
Cited by
Initial sequencing and analysis of the human genome
International Human Genome Sequencing Consortium, Whitehead Institute for Biomedical Research, C. F. G. R., Lander, E. S., Linton, L. M., Birren, B., Nusbaum, C., Zody, M. C., Baldwin, J., Devon, K., Dewar, K., et al. (2001) · Nature · doi:10.1038/35057062 · Cited by 24,216
Anderson, S., de Bruijn, M. H. L., Coulson, A. R., Eperon, I. C., Sanger, F., & Young, I. G. (1982) · Journal of Molecular Biology · doi:10.1016/0022-2836(82)90137-1 · Cited by 1,472
Self-citationThe sequence of sequencers: The history of sequencing DNA
Heather, J. M., & Chain, B. (2016) · Genomics · doi:10.1016/j.ygeno.2015.11.003 · Cited by 1,397
“…than one kilobase (kb) in length: in order to analyse longer fragments researchers made use of techniques such as ‘ shotgun sequencing ’ where overlapping DNA fragments were cloned and sequenced separately, and then assembled into one long contiguous sequence (or ‘ contig ’ ) in silico [35,36].”
BackgroundHolo, H., & Nes, I. F. (1989) · Applied and Environmental Microbiology · doi:10.1128/aem.55.12.3119-3123.1989 · Cited by 1,048
O'Hare, K., & Rubin, G. M. (1983) · Cell · doi:10.1016/0092-8674(83)90133-2 · Cited by 784
Metadata
- Authors
- Anderson, S.
- Venue
- Nucleic Acids Research
- Publisher
- Oxford University Press (OUP)
- Published
- 1981
- Volume
- 9
- Issue
- 13
- Pages
- 3015-3027
- ISSN
- 0305-1048 (ISSN-L)
- 1362-4962
- OpenAlex
- W2125196454
- Cited by
- 419
- References
- 22
- FWCI
- 8.97
- Type
- journal article
- Language
- en
- Open access
- bronze
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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