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High-Frequency Transformation, by Electroporation, of Lactococcus lactis subsp. cremoris Grown with Glycine in Osmotically Stabilized Media
Open accessAbstract
An efficient method for genetic transformation of lactococci by electroporation is presented. Highly competent lactococci for electrotransformation were obtained by growing cells in media containing high concentrations of glycine and 0.5 M sucrose as the osmotic stabilizers. These cells could be stored at −85°C without loss of competence. With Lactococcus lactis subsp. cremoris BC101, a transformation frequency of 5.7 × 10 7 transformants per μg of pIL253 DNA was obtained, which represents 5% of the surviving cells. All the lactococcal strains tested could be transformed by the present method.
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Citations
Cited by
Microbial Cellulose Utilization: Fundamentals and Biotechnology
Lynd, L. R., Weimer, P. J., van Zyl, W. H., & Pretorius, I. S. (2002) · Microbiology and Molecular Biology Reviews · doi:10.1128/mmbr.66.3.506-577.2002 · Cited by 4,726
Buist, G., Kok, J., Leenhouts, K. J., Dabrowska, M., Venema, G., & Haandrikman, A. J. (1995) · Journal of Bacteriology · doi:10.1128/jb.177.6.1554-1563.1995 · Cited by 247
Genetic and biochemical characterization of the oligopeptide transport system of Lactococcus lactis
Tynkkynen, S., Buist, G., Kunji, E., Kok, J., Poolman, B., Venema, G., & Haandrikman, A. (1993) · Journal of Bacteriology · doi:10.1128/jb.175.23.7523-7532.1993 · Cited by 231
Yvon, M., Chambellon, E., Bolotin, A., & Roudot-Algaron, F. (2000) · Applied and Environmental Microbiology · doi:10.1128/aem.66.2.571-577.2000 · Cited by 176
Kjos, M., Salehian, Z., Nes, I. F., & Diep, D. B. (2010) · Journal of Bacteriology · doi:10.1128/jb.00777-10 · Cited by 121
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Metadata
- Authors
- Holo, H., & Nes, I. F.
- Venue
- Applied and Environmental Microbiology
- Publisher
- American Society for Microbiology
- Published
- 1989-12
- Volume
- 55
- Issue
- 12
- Pages
- 3119-3123
- ISSN
- 0099-2240 (ISSN-L)
- 1098-5336
- OpenAlex
- W2109276669
- Cited by
- 1,048
- References
- 0
- FWCI
- 4.40
- Type
- journal article
- Language
- en
- Open access
- green
- Licence
- journals.asm.org/non-commercial-tdm-license (tdm)
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- Holo, H., & Nes, I. F. (1989). High-Frequency Transformation, by Electroporation, of Lactococcus lactis subsp. cremoris Grown with Glycine in Osmotically Stabilized Media. Applied and Environmental Microbiology. https://doi.org/10.1128/aem.55.12.3119-3123.1989
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Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
