The DOI matches its Crossref record; 30 of the 30 references it cites were found in a registry or an index.
A small segment of polyoma virus DNA enhances the expression of a cloned β-globin gene over a distance of 1400 base pairs
Open accessAbstract
The hemoglobin beta-1 gene of the rabbit was linked to a 244 bp DNA fragment from the beginning of the polyoma virus late region, not including the viral origin of replication. After transfection of such recombinant DNAs into mouse 3T6 and human HeLa cells, the polyoma sequences were found to strongly enhance the level of correct beta-globin gene transcripts over a distance of at least 1400 bp. These findings are similar to those obtained with a segment of DNA from the corresponding region of the SV40 genome (J. Banerji, S. Rusconi and W. Schaffner, 1981, Cell, in press) which, however, shows very limited sequence homology to the polyoma 244 bp segment. Using the same assay, a complete copy of polyoma virus DNA was found to interfere with the enhancement of globin gene expression in a cell type-specific manner which may be due to incorrect transcription. In contrast to the complete polyoma virus genome, the 244 bp DNA fragment will be particularly useful as a component of mammalian expression vectors since it almost exclusively yielded high levels of correct beta-globin gene transcripts.
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Cites
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SV40-transformed simian cells support the replication of early SV40 mutants
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The isolation of structural genes from libraries of eucaryotic DNA
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Cited by
Gillies, S. D., Morrison, S. L., Oi, V. T., & Tonegawa, S. (1983) · Cell · doi:10.1016/0092-8674(83)90014-4 · Cited by 1,361
Two distinct enhancers with different cell specificities coexist in the regulatory region of polyoma
Herbomel, P., Bourachot, B., & Yaniv, M. (1984) · Cell · doi:10.1016/0092-8674(84)90472-0 · Cited by 837
Multiple Point Mutations Affecting the Simian Virus 40 Enhancer
Weiher, H., König, M., & Gruss, P. (1983) · Science · doi:10.1126/science.6297005 · Cited by 832
Treisman, R. (1985) · Cell · doi:10.1016/0092-8674(85)90285-5 · Cited by 816
Human polyomavirus JC virus genome
Frisque, R. J., Bream, G. L., & Cannella, M. T. (1984) · Journal of Virology · doi:10.1128/jvi.51.2.458-469.1984 · Cited by 675
Metadata
- Authors
- de Villiers, J., & Schaffner, W.
- Venue
- Nucleic Acids Research
- Publisher
- Oxford University Press (OUP)
- Published
- 1981
- Volume
- 9
- Issue
- 23
- Pages
- 6251-6264
- ISSN
- 0305-1048 (ISSN-L)
- 1362-4962
- OpenAlex
- W1971420993
- Cited by
- 321
- References
- 30
- FWCI
- 21.32
- Type
- journal article
- Language
- en
- Open access
- green
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- de Villiers, J., & Schaffner, W. (1981). A small segment of polyoma virus DNA enhances the expression of a cloned β-globin gene over a distance of 1400 base pairs. Nucleic Acids Research. https://doi.org/10.1093/nar/9.23.6251
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
