Cited by 30RNA and protein synthesis mechanismsMitochondrial Function and PathologyNuclear Structure and Function

The translational activator Sov1 coordinates mitochondrial gene expression with mitoribosome biogenesis

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Seshadri, Suhas R, Banarjee, Chitra, Barros, Mario H, Fontanesi, Flavia·2020·Nucleic Acids Research

Abstract

Mitoribosome biogenesis is an expensive metabolic process that is essential to maintain cellular respiratory capacity and requires the stoichiometric accumulation of rRNAs and proteins encoded in two distinct genomes. In yeast, the ribosomal protein Var1, alias uS3m, is mitochondrion-encoded. uS3m is a protein universally present in all ribosomes, where it forms part of the small subunit (SSU) mRNA entry channel and plays a pivotal role in ribosome loading onto the mRNA. However, despite its critical functional role, very little is known concerning VAR1 gene expression. Here, we demonstrate that the protein Sov1 is an in bona fide VAR1 mRNA translational activator and additionally interacts with newly synthesized Var1 polypeptide. Moreover, we show that Sov1 assists the late steps of mtSSU biogenesis involving the incorporation of Var1, an event necessary for uS14 and mS46 assembly. Notably, we have uncovered a translational regulatory mechanism by which Sov1 fine-tunes Var1 synthesis with its assembly into the mitoribosome.

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  • The shift from transcriptional control to posttranscriptional regulation is evolutionarily advanced compared to prokaryotic ancestors that lack PPR proteins.

    “The shift from transcriptional control to posttranscriptional regulation is considered evolutionarily advanced compared to prokaryotic ancestors which do not contain PPR proteins (Ott et al. 2016; Siira et al. 2017; Seshadri et al. 2020).”

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  1. [19]Sanchirico M. Understandingmitochondrial biogenesis through gene relocation. 1998; Amherst University of Massachussetts PhD thesis.

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Authors
Seshadri, S. R., Banarjee, C., Barros, M. H., & Fontanesi, F.
Affiliations
  • University of Miami (US)
  • Universidade de São Paulo (BR)
Corresponding author
Fontanesi, Flavia
Venue
Nucleic Acids Research
Published
2020-05-25
Volume
48
Issue
12
Pages
6759-6774
ISSN
  • 0305-1048 (ISSN-L)
  • 1362-4962
OpenAlex
W3031328592
Cited by
30
References
61
Citations by year
2026: 4 · 2025: 4 · 2024: 5 · 2023: 7 · 2022: 3 · 2021: 4 · 2020: 3
FWCI
1.38
Type
journal article
Language
en
Open access
Locations
  • Nucleic Acids Research (publishedVersion, cc-by-nc)
  • PubMed Central (publishedVersion, cc-by-nc)
  • PubMed (publishedVersion)
Funders
  • São Paulo Research Foundation (FAPESP 2017/23921-5)
  • U.S. Department of Defense (PR180598)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA and protein synthesis mechanisms
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Mitochondrial Function and Pathology
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Nuclear Structure and Function
Keywords
mitochondrial gene expression, translational regulation, ribosome loading, Saccharomyces cerevisiae
MeSH terms
Mitochondrial Ribosomes, DNA, Mitochondrial, Membrane Proteins, Ribosomal Proteins, RNA, Messenger, Saccharomyces cerevisiae, Gene Expression Regulation, Fungal, Mitochondrial Proteins, Saccharomyces cerevisiae Proteins

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