Cited by 65Mitochondrial Function and PathologyBiotin and Related StudiesFungal and yeast genetics research

Molecular connectivity of mitochondrial gene expression and OXPHOS biogenesis

Open access
Singh, Abeer Prakash, Salvatori, Roger, Aftab, Wasim, Kohler, Andreas, Carlström, Andreas, Forne, Ignasi, Imhof, Axel, Ott, Martin·2020·Molecular Cell

Abstract

Mitochondria contain their own gene expression systems, including membrane-bound ribosomes dedicated to synthesizing a few hydrophobic subunits of the oxidative phosphorylation (OXPHOS) complexes. We used a proximity-dependent biotinylation technique, BioID, coupled with mass spectrometry to delineate in baker's yeast a comprehensive network of factors involved in biogenesis of mitochondrial encoded proteins. This mitochondrial gene expression network (MiGENet) encompasses proteins involved in transcription, RNA processing, translation, or protein biogenesis. Our analyses indicate the spatial organization of these processes, thereby revealing basic mechanistic principles and the proteins populating strategically important sites. For example, newly synthesized proteins are directly handed over to ribosomal tunnel exit-bound factors that mediate membrane insertion, co-factor acquisition, or their mounting into OXPHOS complexes in a special early assembly hub. Collectively, the data reveal the connectivity of mitochondrial gene expression, reflecting a unique tailoring of the mitochondrial gene expression system.

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Crossref · 10.1016/j.molcel.2020.07.024

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  • Transcription and translation in fungal mitochondria are closely connected by the RNA processing machinery.

    “Transcription and translation in fungal mitochondria are believed to be closely connected by the RNA processing machinery (Singh et al. 2020), which partially explains the lower level of multiple mitochondrial transcripts in the Cappr7Δ strain.”

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Authors
Singh, A. P., Salvatori, R., Aftab, W., Kohler, A., Carlström, A., Forne, I., Imhof, A., & Ott, M.
Affiliations
  • Stockholm University (SE)
  • Ludwig-Maximilians-Universität München (DE)
Corresponding author
Ott, Martin
Venue
Molecular Cell
Publisher
Elsevier BV
Published
2020-09
Volume
79
Issue
6
Pages
1051-1065.e10
ISSN
1097-2765 (ISSN-L)
OpenAlex
W3082520945
Cited by
65
References
98
Citations by year
2026: 10 · 2025: 9 · 2024: 13 · 2023: 15 · 2022: 8 · 2021: 9 · 2020: 1
FWCI
2.67
Type
journal article
Language
en
Open access
Locations
  • Molecular Cell (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • KTH Publication Database DiVA (KTH Royal Institute of Technology) (submittedVersion, other-oa)
Funders
  • Vetenskapsrådet
  • Ludwig-Maximilians-Universität München
  • Knut och Alice Wallenbergs Stiftelse (J4398-B)
  • Carl Tryggers Stiftelse för Vetenskaplig Forskning
  • Austrian Science Fund
  • Wenner-Gren Stiftelserna
  • Deutsche Forschungsgemeinschaft (RI-00089)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Mitochondrial Function and Pathology
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Biotin and Related Studies
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
Keywords
mitochondrial gene expression, mitochondrial ribosome, protein-protein interaction network, membrane insertion, Saccharomyces cerevisiae
MeSH terms
Membrane Proteins, Mitochondria, Oxidative Phosphorylation, Ribosomal Proteins, Saccharomyces cerevisiae, Protein Biosynthesis, Gene Expression Regulation, Fungal, Mitochondrial Proteins, Saccharomyces cerevisiae Proteins

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