Molecular connectivity of mitochondrial gene expression and OXPHOS biogenesis
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Mitochondria contain their own gene expression systems, including membrane-bound ribosomes dedicated to synthesizing a few hydrophobic subunits of the oxidative phosphorylation (OXPHOS) complexes. We used a proximity-dependent biotinylation technique, BioID, coupled with mass spectrometry to delineate in baker's yeast a comprehensive network of factors involved in biogenesis of mitochondrial encoded proteins. This mitochondrial gene expression network (MiGENet) encompasses proteins involved in transcription, RNA processing, translation, or protein biogenesis. Our analyses indicate the spatial organization of these processes, thereby revealing basic mechanistic principles and the proteins populating strategically important sites. For example, newly synthesized proteins are directly handed over to ribosomal tunnel exit-bound factors that mediate membrane insertion, co-factor acquisition, or their mounting into OXPHOS complexes in a special early assembly hub. Collectively, the data reveal the connectivity of mitochondrial gene expression, reflecting a unique tailoring of the mitochondrial gene expression system.
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Transcription and translation in fungal mitochondria are closely connected by the RNA processing machinery.
“Transcription and translation in fungal mitochondria are believed to be closely connected by the RNA processing machinery (Singh et al. 2020), which partially explains the lower level of multiple mitochondrial transcripts in the Cappr7Δ strain.”
From <i>Candida albicans</i> PPR proteins are required for the expression of respiratory Complex I subunits (2024) · cites this paper as Singh et al. 2020
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Li, P., Meng, Y., Wang, L., et al. (2018) · Methods in molecular biology · doi:10.1007/978-1-4939-8814-3_10 · Cited by 18 · see citing works
Watts, T., Khalimonchuk, O., Wolf, R. Z., Turk, E. M., Mohr, G., & Winge, D. R. (2011) · Journal of Biological Chemistry · doi:10.1074/jbc.m110.205625 · Cited by 16 · see citing works
Franco, L. V. R., Moda, B. S., Soares, M. A. K. M., & de Barros, M. H. (2019) · FEBS Journal · doi:10.1111/febs.14785 · Cited by 9 · see citing works
Moreno, J. I., Coleman, I. S., Johnson, C. L., Green, D. S., & Piva, M. A. (2020) · Current Genetics · doi:10.1007/s00294-020-01064-0 · Cited by 6 · see citing works
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Hillen, H. S., Markov, D. A., Wojtas, I. D., Hofmann, K. B., Lidschreiber, M., Cowan, A. T., Jones, J. L., Temiakov, D., Cramer, P., & Anikin, M. (2021) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.2009329118 · Cited by 21 · see citing works
Wenda, J. M., Drzewicka, K., Mulica, P., Tetaud, E., di Rago, J. P., Golik, P., & Łabędzka-Dmoch, K. (2024) · GENETICS · doi:10.1093/genetics/iyae124 · Cited by 2 · see citing works
- Authors
- Singh, A. P., Salvatori, R., Aftab, W., Kohler, A., Carlström, A., Forne, I., Imhof, A., & Ott, M.
- Affiliations
- Stockholm University (SE)
- Ludwig-Maximilians-Universität München (DE)
- Corresponding author
- Ott, Martin
- Venue
- Molecular Cell
- Publisher
- Elsevier BV
- Published
- 2020-09
- Volume
- 79
- Issue
- 6
- Pages
- 1051-1065.e10
- ISSN
- 1097-2765 (ISSN-L)
- OpenAlex
- W3082520945
- Cited by
- 65
- References
- 98
- Citations by year
- 2026: 10 · 2025: 9 · 2024: 13 · 2023: 15 · 2022: 8 · 2021: 9 · 2020: 1
- FWCI
- 2.67
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- Molecular Cell (publishedVersion, cc-by)
- PubMed (publishedVersion)
- KTH Publication Database DiVA (KTH Royal Institute of Technology) (submittedVersion, other-oa)
- Funders
- Vetenskapsrådet
- Ludwig-Maximilians-Universität München
- Knut och Alice Wallenbergs Stiftelse (J4398-B)
- Carl Tryggers Stiftelse för Vetenskaplig Forskning
- Austrian Science Fund
- Wenner-Gren Stiftelserna
- Deutsche Forschungsgemeinschaft (RI-00089)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Mitochondrial Function and Pathology
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Biotin and Related Studies
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
- Keywords
- mitochondrial gene expression, mitochondrial ribosome, protein-protein interaction network, membrane insertion, Saccharomyces cerevisiae
- MeSH terms
- Membrane Proteins, Mitochondria, Oxidative Phosphorylation, Ribosomal Proteins, Saccharomyces cerevisiae, Protein Biosynthesis, Gene Expression Regulation, Fungal, Mitochondrial Proteins, Saccharomyces cerevisiae Proteins
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
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