The DOI matches its Crossref record; 93 of the 93 references it cites were found in a registry or an index.
Expanding the coverage of regulons from high-confidence prior knowledge for accurate estimation of transcription factor activities
Open accessAbstract
Gene regulation plays a critical role in the cellular processes that underlie human health and disease. The regulatory relationship between transcription factors (TFs), key regulators of gene expression, and their target genes, the so called TF regulons, can be coupled with computational algorithms to estimate the activity of TFs. However, to interpret these findings accurately, regulons of high reliability and coverage are needed. In this study, we present and evaluate a collection of regulons created using the CollecTRI meta-resource containing signed TF–gene interactions for 1186 TFs. In this context, we introduce a workflow to integrate information from multiple resources and assign the sign of regulation to TF–gene interactions that could be applied to other comprehensive knowledge bases. We find that the signed CollecTRI-derived regulons outperform other public collections of regulatory interactions in accurately inferring changes in TF activities in perturbation experiments. Furthermore, we showcase the value of the regulons by examining TF activity profiles in three different cancer types and exploring TF activities at the level of single-cells. Overall, the CollecTRI-derived TF regulons enable the accurate and comprehensive estimation of TF activities and thereby help to interpret transcriptomics data.
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Cites
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Self-citationThe Human Transcription Factors
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“Transcription factor activity was computed at both the nuclei and spot level using the Col-lecTRI 79 resource with the method ULM from decoupler 73 .”
Self-citationBenchmarking foundation cell models for post-perturbation RNA-seq prediction
Csendes, G., Sanz, G., Szalay, K. Z., & Szalai, B. (2025) · BMC Genomics · doi:10.1186/s12864-025-11600-2 · Cited by 47
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Ag-driven CD8+ T cell clonal expansion is a prominent feature of MASH in humans and mice
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Metadata
- Authors
- Müller-Dott, S., Tsirvouli, E., Vazquez, M., Ramirez Flores, R. O., Badia-i-Mompel, P., Fallegger, R., Türei, D., Lægreid, A., & Saez-Rodriguez, J.
- Venue
- Nucleic Acids Research
- Publisher
- Oxford University Press (OUP)
- Published
- 2023-10-16
- Volume
- 51
- Issue
- 20
- Pages
- 10934-10949
- ISSN
- 0305-1048 (ISSN-L)
- 1362-4962
- OpenAlex
- W4387661643
- Cited by
- 380
- References
- 93
- FWCI
- 28.39
- Type
- journal article
- Language
- en
- Open access
- gold
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- Müller-Dott, S., Tsirvouli, E., Vazquez, M., Ramirez Flores, R. O., Badia-i-Mompel, P., Fallegger, R., Türei, D., Lægreid, A., & Saez-Rodriguez, J. (2023). Expanding the coverage of regulons from high-confidence prior knowledge for accurate estimation of transcription factor activities. Nucleic Acids Research. https://doi.org/10.1093/nar/gkad841
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