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Cited by 200Chromosomal and Genetic VariationsGenomics and Chromatin DynamicsRNA and protein synthesis mechanisms

A YY1-binding site is required for accurate human LINE-1 transcription initiation

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Athanikar, J. N.·2004·Nucleic Acids Research
DOI 10.1093/nar/gkh698PMC PMC506791
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Abstract

The initial step in Long Interspersed Element-1 (LINE-1) retrotransposition requires transcription from an internal promoter located within its 5'-untranslated region (5'-UTR). Previous studies have identified a YY1 (Yin Yang 1)-binding site as an important sequence in LINE-1 transcription. Here, we demonstrate that mutations in the YY1-binding site have only minor effects on transcription activation of the full-length 5'-UTR and LINE-1 mobility in a single round cultured cell retrotransposition assay. Instead, these mutations disrupt proper initiation of transcription from the +1 site of the 5'-UTR. Thus, we propose that the YY1-binding site functions as a component of the LINE-1 core promoter to direct accurate transcription initiation. Indeed, this sequence may explain the evolutionary success of LINE-1 by enabling full-length retrotransposed copies to undergo autonomous retrotransposition in subsequent generations.

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Authors
Athanikar, J. N.
Affiliations
University of Michigan (US)
Corresponding author
Athanikar, J. N.
Venue
Nucleic Acids Research
Publisher
Oxford University Press (OUP)
Published
2004-07-16
Volume
32
Issue
13
Pages
3846-3855
ISSN
  • 0305-1048 (ISSN-L)
  • 1362-4962
DOI
10.1093/nar/gkh698
PMC
PMC506791
OpenAlex
W2158882685
Semantic Scholar
0ff7bef52a47a2ef0690468a408e8a32629c3817
Cited by
200
References
54
Citations by year
2026: 12 · 2025: 5 · 2024: 11 · 2023: 14 · 2022: 14 · 2021: 10 · 2020: 6 · 2019: 10 · 2018: 11 · 2017: 8 · 2016: 12 · 2015: 12 · 2014: 6 · 2013: 8 · 2012: 7
FWCI
10.16
Type
journal article
Language
en
Open access
Locations
  • Nucleic Acids Research (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
  • Figshare (submittedVersion, cc-by)
  • Leicester Research Archive (University of Leicester) (submittedVersion)
Funders
  • University of Michigan
  • Wellcome Trust (069702/Z/02/Z)
  • National Institutes of Health
  • National Cancer Institute (CA009676, T32CA009676)
  • National Institute of General Medical Sciences (GM60518, F32GM20859, R01GM060518, F32GM020859)
Topics
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Chromosomal and Genetic Variations
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA and protein synthesis mechanisms
Keywords
LINE-1, YY1 binding site, transcription initiation, core promoter, 5' untranslated region, retrotransposition, transcriptional activation
MeSH terms
Base Sequence, Binding Sites, Humans, Molecular Sequence Data, Mutation, Transcription Factors, Transcription, Genetic, Transcriptional Activation, Mutagenesis, Insertional, Long Interspersed Nucleotide Elements, 5' Untranslated Regions, Cell Line, Tumor, Erythroid-Specific DNA-Binding Factors, YY1 Transcription Factor

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