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Cited by 405

The DOI matches its Crossref record; 47 of the 47 references it cites were found in a registry or an index.

Identification of the CRP regulon using in vitro and in vivo transcriptional profiling

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Zheng, D.·2004·Nucleic Acids Research
DOI 10.1093/nar/gkh908
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Abstract

The Escherichia coli cyclic AMP receptor protein (CRP) is a global regulator that controls transcription initiation from more than 100 promoters by binding to a specific DNA sequence within cognate promoters. Many genes in the CRP regulon have been predicted simply based on the presence of DNA-binding sites within gene promoters. In this study, we have exploited a newly developed technique, run-off transcription/microarray analysis (ROMA) to define CRP-regulated promoters. Using ROMA, we identified 176 operons that were activated by CRP in vitro and 16 operons that were repressed. Using positive control mutants in different regions of CRP, we were able to classify the different promoters into class I or class II/III. A total of 104 operons were predicted to contain Class II CRP-binding sites. Sequence analysis of the operons that were repressed by CRP revealed different mechanisms for CRP inhibition. In contrast, the in vivo transcriptional profiles failed to identify most CRP-dependent regulation because of the complexity of the regulatory network. Analysis of these operons supports the hypothesis that CRP is not only a regulator of genes required for catabolism of sugars other than glucose, but also regulates the expression of a large number of other genes in E.coli. ROMA has revealed 152 hitherto unknown CRP regulons.

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Metadata

Authors
Zheng, D.
Venue
Nucleic Acids Research
Publisher
Oxford University Press (OUP)
Published
2004-10-28
Volume
32
Issue
19
Pages
5874-5893
ISSN
  • 0305-1048 (ISSN-L)
  • 1362-4962
DOI
10.1093/nar/gkh908
OpenAlex
W1994782227
Cited by
405
References
47
FWCI
7.75
Type
journal article
Language
en
Open access
bronze

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CoChat. (2026). Citation check: Identification of the CRP regulon using in vitro and in vivo transcriptional profiling. https://app.cochat.ai/papers/10.1093/nar/gkh908
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Zheng, D. (2004). Identification of the CRP regulon using in vitro and in vivo transcriptional profiling. Nucleic Acids Research. https://doi.org/10.1093/nar/gkh908

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