The DOI matches its Crossref record; 40 of the 40 references it cites were found in a registry or an index.
Oxidative damage in telomeric DNA disrupts recognition by TRF1 and TRF2
Open accessAbstract
The ends of linear chromosomes are capped by protein-DNA complexes termed telomeres. Telomere repeat binding factors 1 and 2 (TRF1 and TRF2) bind specifically to duplex telomeric DNA and are critical components of functional telomeres. Consequences of telomere dysfunction include genomic instability, cellular apoptosis or senescence and organismal aging. Mild oxidative stress induces increased erosion and loss of telomeric DNA in human fibroblasts. We performed binding assays to determine whether oxidative DNA damage in telomeric DNA alters the binding activity of TRF1 and TRF2 proteins. Here, we report that a single 8-oxo-guanine lesion in a defined telomeric substrate reduced the percentage of bound TRF1 and TRF2 proteins by at least 50%, compared with undamaged telomeric DNA. More dramatic effects on TRF1 and TRF2 binding were observed with multiple 8-oxo-guanine lesions in the tandem telomeric repeats. Binding was likewise disrupted when certain intermediates of base excision repair were present within the telomeric tract, namely abasic sites or single nucleotide gaps. These studies indicate that oxidative DNA damage may exert deleterious effects on telomeres by disrupting the association of telomere-maintenance proteins TRF1 and TRF2.
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“In addition, 8-oxo-Gs accumulate at telomeres, where they inhibit telomerase and decrease the binding of telomeric proteins, leading to the disruption of telomere length and precluding the maintenance of chromosomal-end capping46.”
From ROS in cancer therapy: the bright side of the moon (2020) · cites this paper as [46]
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“Oxidative stress reportedly both induces tDD without telomere shortening and accelerates telomere shortening23–26 by inhibiting telomerase27 and disrupting the recognition by telomere-binding proteins, which contributes to telomere uncapping22,28.”
From Telomere dysfunction in ageing and age-related diseases (2022) · cites this paper as [28]
In the citing paper
“oxidative damage at telomeres displaces shelterin proteins TRF1 and TRF2, which might be another mechanism by which oxidative stress leads to telomere dysfunction (Opresko et al., 2005).”
From Telomeres and cell senescence—size matters not (2017) · cites this paper as Opresko et al., 2005
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Citations
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Metadata
- Authors
- Opresko, P. L.
- Affiliations
- National Institutes of Health (US)
- Corresponding author
- Opresko, P. L.
- ORCID
- Opresko, P. L.
- Venue
- Nucleic Acids Research
- Publisher
- Oxford University Press (OUP)
- Published
- 2005-02-23
- Volume
- 33
- Issue
- 4
- Pages
- 1230-1239
- ISSN
- 0305-1048 (ISSN-L)
- 1362-4962
- PMC
- PMC549571
- OpenAlex
- W2098312065
- Cited by
- 289
- References
- 40
- Citations by year
- 2026: 10 · 2025: 15 · 2024: 10 · 2023: 17 · 2022: 19 · 2021: 20 · 2020: 23 · 2019: 18 · 2018: 15 · 2017: 14 · 2016: 20 · 2015: 20 · 2014: 17 · 2013: 14 · 2012: 6
- FWCI
- 4.61
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- other-oa
- Locations
- Nucleic Acids Research (publishedVersion, other-oa)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- eScholarship (California Digital Library) (submittedVersion)
- Funders
- National Institutes of Health
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Telomeres, Telomerase, and Senescence
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › DNA and Nucleic Acid Chemistry
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › DNA Repair Mechanisms
- Keywords
- telomeres, TRF1, TRF2, oxidative DNA damage, 8-oxoguanine, telomeric DNA binding, base excision repair, abasic sites, telomere dysfunction, telomere shortening
- MeSH terms
- DNA, DNA Damage, Guanine, Humans, Telomere, Oxidative Stress, Telomeric Repeat Binding Protein 1, Telomeric Repeat Binding Protein 2
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