The DOI matches its Crossref record; 40 of the 40 references it cites were found in a registry or an index.
Oxidative damage in telomeric DNA disrupts recognition by TRF1 and TRF2
Open accessAbstract
The ends of linear chromosomes are capped by protein-DNA complexes termed telomeres. Telomere repeat binding factors 1 and 2 (TRF1 and TRF2) bind specifically to duplex telomeric DNA and are critical components of functional telomeres. Consequences of telomere dysfunction include genomic instability, cellular apoptosis or senescence and organismal aging. Mild oxidative stress induces increased erosion and loss of telomeric DNA in human fibroblasts. We performed binding assays to determine whether oxidative DNA damage in telomeric DNA alters the binding activity of TRF1 and TRF2 proteins. Here, we report that a single 8-oxo-guanine lesion in a defined telomeric substrate reduced the percentage of bound TRF1 and TRF2 proteins by at least 50%, compared with undamaged telomeric DNA. More dramatic effects on TRF1 and TRF2 binding were observed with multiple 8-oxo-guanine lesions in the tandem telomeric repeats. Binding was likewise disrupted when certain intermediates of base excision repair were present within the telomeric tract, namely abasic sites or single nucleotide gaps. These studies indicate that oxidative DNA damage may exert deleterious effects on telomeres by disrupting the association of telomere-maintenance proteins TRF1 and TRF2.
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“In addition, 8-oxo-Gs accumulate at telomeres, where they inhibit telomerase and decrease the binding of telomeric proteins, leading to the disruption of telomere length and precluding the maintenance of chromosomal-end capping46.”
From ROS in cancer therapy: the bright side of the moon (2020) · cites this paper as [46]
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“Oxidative stress reportedly both induces tDD without telomere shortening and accelerates telomere shortening23–26 by inhibiting telomerase27 and disrupting the recognition by telomere-binding proteins, which contributes to telomere uncapping22,28.”
From Telomere dysfunction in ageing and age-related diseases (2022) · cites this paper as [28]
In the citing paper
“oxidative damage at telomeres displaces shelterin proteins TRF1 and TRF2, which might be another mechanism by which oxidative stress leads to telomere dysfunction (Opresko et al., 2005).”
From Telomeres and cell senescence—size matters not (2017) · cites this paper as Opresko et al., 2005
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Citations
Cites
Instability and decay of the primary structure of DNA
Lindahl, T. (1993) · Nature · doi:10.1038/362709a0 · Cited by 5,679
Oxidative stress shortens telomeres
von Zglinicki, T. (2002) · Trends in Biochemical Sciences · doi:10.1016/s0968-0004(02)02110-2 · Cited by 2,524
Mammalian Telomeres End in a Large Duplex Loop
Griffith, J. D., Comeau, L., Rosenfield, S., Stansel, R. M., Bianchi, A., Moss, H., & de Lange, T. (1999) · Cell · doi:10.1016/s0092-8674(00)80760-6 · Cited by 2,447
Insertion of specific bases during DNA synthesis past the oxidation-damaged base 8-oxodG
Shibutani, S., Takeshita, M., & Grollman, A. P. (1991) · Nature · doi:10.1038/349431a0 · Cited by 2,224
TRF2 Protects Human Telomeres from End-to-End Fusions
van Steensel, B., Smogorzewska, A., & de Lange, T. (1998) · Cell · doi:10.1016/s0092-8674(00)80932-0 · Cited by 1,709
Protection of mammalian telomeres
de Lange, T. (2002) · Oncogene · doi:10.1038/sj.onc.1205080 · Cited by 942
Control of Human Telomere Length by TRF1 and TRF2
Smogorzewska, A., van Steensel, B., Bianchi, A., Oelmann, S., Schaefer, M. R., Schnapp, G., & de Lange, T. (2000) · Molecular and Cellular Biology · doi:10.1128/mcb.20.5.1659-1668.2000 · Cited by 768
Li, B., Oestreich, S. C., & de Lange, T. (2000) · Cell · doi:10.1016/s0092-8674(00)80858-2 · Cited by 609
T‐loop assembly in vitro involves binding of TRF2 near the 3′ telomeric overhang
Stansel, R. M., de Lange, T., & Griffith, J. D. (2001) · The EMBO Journal · doi:10.1093/emboj/20.19.5532 · Cited by 562
Clustered DNA damages induced in isolated DNA and in human cells by low doses of ionizing radiation
Sutherland, B. M., Bennett, P. V., Sidorkina, O. M., & Laval, J. (2000) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.97.1.103 · Cited by 473
Cited by
Sorted by citations
Cancer-related inflammation, the seventh hallmark of cancer: links to genetic instability
Colotta, F., Allavena, P., Sica, A., Garlanda, C., & Mantovani, A. (2009) · Carcinogenesis · doi:10.1093/carcin/bgp127 · Cited by 2,960
ROS in cancer therapy: the bright side of the moon
Perillo, B., Di Donato, M., Pezone, A., Di Zazzo, E., Giovannelli, P., Galasso, G., Castoria, G., & Migliaccio, A. (2020) · Experimental & Molecular Medicine · doi:10.1038/s12276-020-0384-2 · Cited by 2,176
Oxidative Stress, Mitochondrial Dysfunction, and Aging
Cui, H., Kong, Y., & Zhang, H. (2011) · Journal of Signal Transduction · doi:10.1155/2012/646354 · Cited by 1,076
Hewitt, G., Jurk, D., Marques, F. D. M., Correia-Melo, C., Hardy, T., Gackowska, A., Anderson, R., Taschuk, M., Mann, J., & Passos, J. F. (2012) · Nature Communications · doi:10.1038/ncomms1708 · Cited by 908
Telomere dysfunction in ageing and age-related diseases
Rossiello, F., Jurk, D., Passos, J. F., & d’Adda di Fagagna, F. (2022) · Nature Cell Biology · doi:10.1038/s41556-022-00842-x · Cited by 754
Metadata
- Authors
- Opresko, P. L.
- Affiliations
- National Institutes of Health (US)
- Corresponding author
- Opresko, P. L.
- ORCID
- Opresko, P. L.
- Venue
- Nucleic Acids Research
- Publisher
- Oxford University Press (OUP)
- Published
- 2005-02-23
- Volume
- 33
- Issue
- 4
- Pages
- 1230-1239
- ISSN
- 0305-1048 (ISSN-L)
- 1362-4962
- PMC
- PMC549571
- OpenAlex
- W2098312065
- Cited by
- 289
- References
- 40
- Citations by year
- 2026: 10 · 2025: 15 · 2024: 10 · 2023: 17 · 2022: 19 · 2021: 20 · 2020: 23 · 2019: 18 · 2018: 15 · 2017: 14 · 2016: 20 · 2015: 20 · 2014: 17 · 2013: 14 · 2012: 6
- FWCI
- 4.61
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- other-oa
- Locations
- Nucleic Acids Research (publishedVersion, other-oa)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- eScholarship (California Digital Library) (submittedVersion)
- Funders
- National Institutes of Health
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Telomeres, Telomerase, and Senescence
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › DNA and Nucleic Acid Chemistry
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › DNA Repair Mechanisms
- Keywords
- telomeres, TRF1, TRF2, oxidative DNA damage, 8-oxoguanine, telomeric DNA binding, base excision repair, abasic sites, telomere dysfunction, telomere shortening
- MeSH terms
- DNA, DNA Damage, Guanine, Humans, Telomere, Oxidative Stress, Telomeric Repeat Binding Protein 1, Telomeric Repeat Binding Protein 2
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