An SOS-regulated operon involved in damage-inducible mutagenesis in Caulobacter crescentus
Open accessAbstract
DNA polymerases of the Y-family, such as Escherichia coli UmuC and DinB, are specialized enzymes induced by the SOS response, which bypass lesions allowing the continuation of DNA replication. umuDC orthologs are absent in Caulobacter crescentus and other bacteria, raising the question about the existence of SOS mutagenesis in these organisms. Here, we report that the C.crescentus dinB ortholog is not involved in damage-induced mutagenesis. However, an operon composed of two hypothetical genes and dnaE2, encoding a second copy of the catalytic subunit of Pol III, is damage inducible in a recA-dependent manner, and is responsible for most ultraviolet (UV) and mitomycin C-induced mutations in C.crescentus. The results demonstrate that the three genes are required for the error-prone processing of DNA lesions. The two hypothetical genes were named imuA and imuB, after inducible mutagenesis. ImuB is similar to proteins of the Y-family of polymerases, and possibly cooperates with DnaE2 in lesion bypass. The mutations arising as a consequence of the activity of the imuAB dnaE2 operon are rather unusual for UV irradiation, including G:C to C:G transversions.
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The genes imuA and imuB induce SOS mutagenesis in Caulobacter crescentus but are absent in E. coli.
“Recently, the genes imuA and imuB have been described that induce SOS mutagenesis in Caulobacter crescentus, but absent in E. coli [332].”
From Molecular Mechanisms of Ultraviolet Radiation‐Induced DNA Damage and Repair (2010) · cites this paper as [332]
The Discussion (page unavailable) states that the SOS-regulated imuAB–dnaE2 operon is responsible for most damage-inducible mutagenesis in Caulobacter crescentus. It contrasts this machinery with that of E. coli, but the passages do not establish that both imuA and imuB are absent from E. coli. (LLM, conf=0.84)
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Molecular Mechanisms of Ultraviolet Radiation‐Induced DNA Damage and Repair
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- Authors
- Galhardo, R. S.
- Affiliations
- Universidade de São Paulo (BR)
- Corresponding author
- Galhardo, R. S.
- ORCID
- Galhardo, R. S.
- Venue
- Nucleic Acids Research
- Publisher
- Oxford University Press (OUP)
- Published
- 2005-04-28
- Volume
- 33
- Issue
- 8
- Pages
- 2603-2614
- ISSN
- 0305-1048 (ISSN-L)
- 1362-4962
- PMC
- PMC1092274
- OpenAlex
- W1983014437
- Cited by
- 125
- References
- 54
- Citations by year
- 2026: 4 · 2025: 4 · 2024: 2 · 2023: 5 · 2022: 6 · 2021: 4 · 2020: 5 · 2019: 9 · 2018: 6 · 2017: 9 · 2016: 4 · 2015: 7 · 2014: 7 · 2013: 5 · 2012: 5
- FWCI
- 3.15
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Locations
- Nucleic Acids Research (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Fundação de Amparo à Pesquisa do Estado de São Paulo
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › DNA Repair Mechanisms
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › Carcinogens and Genotoxicity Assessment
- Keywords
- Caulobacter crescentus, SOS response, Y-family DNA polymerases, UV-induced mutations, error-prone DNA replication, DNA damage bypass
- MeSH terms
- Bacterial Proteins, DNA Damage, DNA Polymerase III, Operon, Phylogeny, SOS Response, Genetics, Ultraviolet Rays, Mutagenesis, Genome, Bacterial, Caulobacter crescentus
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