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The multi-domain protein Np95 connects DNA methylation and histone modification
Open accessAbstract
DNA methylation and histone modifications play a central role in the epigenetic regulation of gene expression and cell differentiation. Recently, Np95 (also known as UHRF1 or ICBP90) has been found to interact with Dnmt1 and to bind hemimethylated DNA, indicating together with genetic studies a central role in the maintenance of DNA methylation. Using in vitro binding assays we observed a weak preference of Np95 and its SRA (SET- and Ring-associated) domain for hemimethylated CpG sites. However, the binding kinetics of Np95 in living cells was not affected by the complete loss of genomic methylation. Investigating further links with heterochromatin, we could show that Np95 preferentially binds histone H3 N-terminal tails with trimethylated (H3K9me3) but not acetylated lysine 9 via a tandem Tudor domain. This domain contains three highly conserved aromatic amino acids that form an aromatic cage similar to the one binding H3K9me3 in the chromodomain of HP1ss. Mutations targeting the aromatic cage of the Np95 tandem Tudor domain (Y188A and Y191A) abolished specific H3 histone tail binding. These multiple interactions of the multi-domain protein Np95 with hemimethylated DNA and repressive histone marks as well as with DNA and histone methyltransferases integrate the two major epigenetic silencing pathways.
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Cites
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Cited by
DNA methylation pathways and their crosstalk with histone methylation
Du, J., Johnson, L. M., Jacobsen, S. E., & Patel, D. J. (2015) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm4043 · Cited by 1,000
Recognition and potential mechanisms for replication and erasure of cytosine hydroxymethylation
Hashimoto, H., Liu, Y., Upadhyay, A. K., Chang, Y., Howerton, S. B., Vertino, P. M., Zhang, X., & Cheng, X. (2012) · Nucleic Acids Research · doi:10.1093/nar/gks155 · Cited by 477
Epigenetic reprogramming in mouse pre-implantation development and primordial germ cells
Saitou, M., Kagiwada, S., & Kurimoto, K. (2012) · Development · doi:10.1242/dev.050849 · Cited by 414
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Epigenetic Mechanisms in Inflammation
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Metadata
- Authors
- Rottach, A., Frauer, C., Pichler, G., Bonapace, I. M., Spada, F., & Leonhardt, H.
- Venue
- Nucleic Acids Research
- Publisher
- Oxford University Press (OUP)
- Published
- 2009-12-21
- Volume
- 38
- Issue
- 6
- Pages
- 1796-1804
- ISSN
- 0305-1048 (ISSN-L)
- 1362-4962
- OpenAlex
- W2039056262
- Cited by
- 164
- References
- 5
- FWCI
- 6.48
- Type
- journal article
- Language
- en
- Open access
- gold
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