A genome-wide library of CB4856/N2 introgression lines of Caenorhabditis elegans
Open accessAbstract
Recombinant inbred lines (RILs) derived from Caenorhabditis elegans wild-type N2 and CB4856 are increasingly being used for mapping genes underlying complex traits. To speed up mapping and gene discovery, introgression lines (ILs) offer a powerful tool for more efficient QTL identification. We constructed a library of 90 ILs, each carrying a single homozygous CB4856 genomic segment introgressed into the genetic background of N2. The ILs were genotyped by 123 single-nucleotide polymorphism (SNP) markers. The proportion of the CB4856 segments in most lines does not exceed 3%, and together the introgressions cover 96% of the CB4856 genome. The value of the IL library was demonstrated by identifying novel loci underlying natural variation in two ageing-related traits, i.e. lifespan and pharyngeal pumping rate. Bin mapping of lifespan resulted in six QTLs, which all have a lifespan-shortening effect on the CB4856 allele. We found five QTLs for the decrease in pumping rate, of which four colocated with QTLs found for average lifespan. This suggests pleiotropic or closely linked QTL associated with lifespan and pumping rate. Overall, the presented IL library provides a versatile resource toward easier and efficient fine mapping and functional analyses of loci and genes underlying complex traits in C. elegans.
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Inbred lines have been generated for C. elegans and, recently, metabolic network models became available.
“Inbred lines have been generated for C. elegans (295) and, recently, metabolic network models became available (296, 297).”
From Metabolic Flexibility as an Adaptation to Energy Resources and Requirements in Health and Disease (2018) · cites this paper as [295]
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Metabolic Flexibility as an Adaptation to Energy Resources and Requirements in Health and Disease
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- Authors
- Doroszuk, A., Snoek, L. B., Fradin, E., Riksen, J., & Kammenga, J.
- Affiliations
- Wageningen University & Research (NL)
- Corresponding author
- Kammenga, Jan
- Venue
- Nucleic Acids Research
- Publisher
- Oxford University Press (OUP)
- Published
- 2009-06-19
- Volume
- 37
- Issue
- 16
- Pages
- e110-e110
- ISSN
- 0305-1048 (ISSN-L)
- 1362-4962
- PMC
- PMC2760803
- OpenAlex
- W2152695013
- Cited by
- 102
- References
- 40
- Citations by year
- 2026: 2 · 2025: 4 · 2024: 1 · 2023: 8 · 2022: 6 · 2021: 6 · 2020: 10 · 2019: 8 · 2018: 9 · 2017: 8 · 2016: 7 · 2015: 6 · 2014: 6 · 2013: 8 · 2012: 5
- FWCI
- 2.52
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- Locations
- Nucleic Acids Research (publishedVersion, cc-by-nc)
- PubMed Central (publishedVersion, cc-by-nc)
- PubMed (publishedVersion)
- Socio-Environmental Systems Modeling (acceptedVersion)
- Data Archiving and Networked Services (DANS) (submittedVersion, other-oa)
- Funders
- European Commission (222936)
- Nederlandse Organisatie voor Wetenschappelijk Onderzoek
- Wageningen University and Research
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Genetic Mapping and Diversity in Plants and Animals
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Aging › Genetics, Aging, and Longevity in Model Organisms
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Genetic and phenotypic traits in livestock
- Keywords
- Caenorhabditis elegans, introgression lines, CB4856, N2 wild-type, recombinant inbred lines, QTL mapping, lifespan, natural genetic variation, aging, fine mapping, SNP markers, pleiotropy
- MeSH terms
- Animals, Chromosome Mapping, Longevity, Pharynx, Genomic Library, Caenorhabditis elegans, Animals, Inbred Strains, Quantitative Trait Loci, Genome, Helminth
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