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Cited by 296RNA and protein synthesis mechanismsRNA modifications and cancerPI3K/AKT/mTOR signaling in cancer

tRNA over-expression in breast cancer and functional consequences

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Pavon-Eternod, Mariana, Gomes, Suzanna, Geslain, Renaud, Dai, Qing, Rosner, Marsha Rich, Pan, Tao·2009·Nucleic Acids Research
DOI 10.1093/nar/gkp787PMC PMC2790902
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Abstract

Increased proliferation and elevated levels of protein synthesis are characteristics of transformed and tumor cells. Though components of the translation machinery are often misregulated in cancers, what role tRNA plays in cancer cells has not been explored. We compare genome-wide tRNA expression in cancer-derived versus non-cancer-derived breast cell lines, as well as tRNA expression in breast tumors versus normal breast tissues. In cancer-derived versus non-cancer-derived cell lines, nuclear-encoded tRNAs increase by up to 3-fold and mitochondrial-encoded tRNAs increase by up to 5-fold. In tumors versus normal breast tissues, both nuclear- and mitochondrial-encoded tRNAs increase up to 10-fold. This tRNA over-expression is selective and coordinates with the properties of cognate amino acids. Nuclear- and mitochondrial-encoded tRNAs exhibit distinct expression patterns, indicating that tRNAs can be used as biomarkers for breast cancer. We also performed association analysis for codon usage-tRNA expression for the cell lines. tRNA isoacceptor expression levels are not geared towards optimal translation of house-keeping or cell line specific genes. Instead, tRNA isoacceptor expression levels may favor the translation of cancer-related genes having regulatory roles. Our results suggest a functional consequence of tRNA over-expression in tumor cells. tRNA isoacceptor over-expression may increase the translational efficiency of genes relevant to cancer development and progression.

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  • A human tRNA microarray has been developed for tRNA quantification and has been applied in tRNA profiling in HIV-1, specific human tissues and tumor cells, and in identifying the complete interactome of tRNA-binding proteins.

    “A human tRNA microarray has been developed for tRNA quantification and has been applied in tRNA profiling in HIV-1 (51), as well as of specific human tissues and tumor cells, and in identifying the complete interactome of tRNA-binding proteins (136 -138).”

    From Role of host tRNAs and aminoacyl-tRNA synthetases in retroviral replication (2019) · cites this paper as [137]

    The Introduction (page not available in our copy) says the authors applied a human tRNA microarray to breast cancer cell lines, breast tumors, and normal breast tissue, supporting the tumor and tissue profiling portion. It describes the array as previously developed, and our copy does not report identifying a complete interactome of tRNA-binding proteins. HIV-1 profiling is attached to a separate citation (51) in the citing sentence. (LLM, conf=0.86)

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Research works printed but not linked: 31

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Authors
Pavon-Eternod, M., Gomes, S., Geslain, R., Dai, Q., Rosner, M. R., & Pan, T.
Affiliations
University of Chicago (US)
Corresponding author
  • Rosner, Marsha Rich
  • Pan, Tao
ORCID
Venue
Nucleic Acids Research
Publisher
Oxford University Press (OUP)
Published
2009-09-25
Volume
37
Issue
21
Pages
7268-7280
ISSN
  • 0305-1048 (ISSN-L)
  • 1362-4962
DOI
10.1093/nar/gkp787
PMC
PMC2790902
OpenAlex
W2112243735
Cited by
296
References
0
Citations by year
2026: 7 · 2025: 10 · 2024: 8 · 2023: 21 · 2022: 22 · 2021: 23 · 2020: 28 · 2019: 25 · 2018: 23 · 2017: 22 · 2016: 29 · 2015: 19 · 2014: 22 · 2013: 8 · 2012: 12
FWCI
3.90
Type
journal article
Language
en
Open access
Licence
Locations
  • Nucleic Acids Research (publishedVersion, cc-by-nc)
  • PubMed Central (publishedVersion, cc-by-nc)
  • PubMed (publishedVersion)
Funders
  • National Institutes of Health (W81XWH)
  • National Institute of General Medical Sciences (T32GM007183)
  • Congressionally Directed Medical Research Programs (W81XWH-07-1-0595)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA and protein synthesis mechanisms
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA modifications and cancer
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › PI3K/AKT/mTOR signaling in cancer
Keywords
breast cancer, mitochondrial tRNA, tRNA isoacceptors, codon usage, translation efficiency, tumor markers, cancer cell lines, protein synthesis
MeSH terms
Breast, Breast Neoplasms, Cell Line, Codon, Female, Humans, Nucleotides, RNA, Transfer, Oligonucleotide Array Sequence Analysis, Gene Expression Profiling, Cell Line, Tumor, Genes, Neoplasm

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