Gcn5 facilitates Pol II progression, rather than recruitment to nucleosome-depleted stress promoters, in Schizosaccharomyces pombe
Open accessAbstract
In the fission yeast, the MAP kinase Sty1 and the transcription factor Atf1 regulate up to 400 genes in response to environmental signals, and both proteins have been shown to bind to their promoters in a stress-dependent manner. In a genetic search, we have isolated the histone H3 acetyltransferase Gcn5, a component of the SAGA complex, as being essential for oxidative stress survival and activation of those genes. Upon stress, Gcn5 is recruited to promoters and coding sequences of stress genes in a Sty1- and Atf1-dependent manner, causing both an enhanced acetylation of histone H3 and nucleosome eviction. Unexpectedly, recruitment of RNA polymerase II (Pol II) is not impaired in Δgcn5 cells. We show here that stress genes display a 400-bp long nucleosome depleted region upstream of the transcription start site even prior to activation. Stress treatment does not alter promoter nucleosome architecture, but induces eviction of the downstream nucleosomes at stress genes, which is not observed in Δgcn5 cells. We conclude that, while Pol II is recruited to nucleosome-free stress promoters in a transcription factor dependent manner, Gcn5 mediates eviction of nucleosomes positioned downstream of promoters, allowing efficient Pol II progression along the genes.
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Gcn5-dependent histone acetylation probably increases binding of T cell receptor-induced factors such as NF-κB or NFAT that matter for Il9 expression after antigen receptor stimulation, or may help general transcription factors including RNA polymerase II.
“Gcn5-dependent histone acetylation likely increases binding of T cell receptor-induced factors such as NF-κB or NFAT that are important for Il9 gene expression following antigen receptor stimulation (27) or may facilitate the function of general transcription factors including RNA polymerase II (49).”
From Gcn5 Is Required for PU.1-Dependent IL-9 Induction in Th9 Cells (2012) · cites this paper as [49]
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Gcn5 Is Required for PU.1-Dependent IL-9 Induction in Th9 Cells
Goswami, R., & Kaplan, M. H. (2012) · The Journal of Immunology · doi:10.4049/jimmunol.1201496 · Cited by 97 · see citing works
“1 or IRF4 binding to Il9, Gcn5-dependent histone acetylation likely increases binding of TCR-induced factors such as NF-kB or NFAT that are important for Il9 gene expression after Ag receptor stimulation (27) or may facilitate the function of general transcription factors including RNA polymerase II (49).”
Background
- Authors
- Sansó, M., Vargas-Pérez, I., Quintales, L., Antequera, F., Ayté, J., & Hidalgo, E.
- Affiliations
- Universitat Pompeu Fabra (ES)
- Corresponding author
- Hidalgo, Elena
- Venue
- Nucleic Acids Research
- Publisher
- Oxford University Press (OUP)
- Published
- 2011-04-22
- Volume
- 39
- Issue
- 15
- Pages
- 6369-6379
- ISSN
- 0305-1048 (ISSN-L)
- 1362-4962
- PMC
- PMC3159446
- OpenAlex
- W2035849174
- Cited by
- 51
- References
- 58
- Citations by year
- 2026: 2 · 2025: 1 · 2024: 1 · 2023: 3 · 2022: 5 · 2021: 1 · 2020: 2 · 2019: 6 · 2018: 3 · 2017: 5 · 2016: 4 · 2015: 2 · 2014: 3 · 2013: 6 · 2012: 5
- FWCI
- 2.08
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- Locations
- Nucleic Acids Research (publishedVersion, cc-by-nc)
- Europe PMC (PubMed Central) (publishedVersion, cc-by-nc)
- PubMed Central (publishedVersion, cc-by-nc)
- PubMed (publishedVersion)
- RECERCAT (Consorci de Serveis Universitaris de Catalunya) (publishedVersion, other-oa)
- DIGITAL.CSIC (Spanish National Research Council (CSIC)) (submittedVersion, cc-by-nc)
- Repositori digital de la UPF (Universitat Pompeu Fabra) (submittedVersion, cc-by-nc)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Nuclear Structure and Function
- Keywords
- GCN5, RNA polymerase II, nucleosome eviction, nucleosome-depleted regions, oxidative stress response, SAGA complex, transcriptional activation, promoter architecture, Schizosaccharomyces pombe
- MeSH terms
- p300-CBP-Associated Factor, Acetylation, Acetyltransferases, Histones, Nucleosomes, Phosphoproteins, Promoter Regions, Genetic, RNA Polymerase II, Schizosaccharomyces, Stress, Physiological, Transcriptional Activation, Gene Expression Regulation, Fungal, Gene Deletion, Mitogen-Activated Protein Kinases, Schizosaccharomyces pombe Proteins, Activating Transcription Factor 1
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