Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe
Open accessAbstract
We report the construction and analysis of 4,836 heterozygous diploid deletion mutants covering 98.4% of the fission yeast genome providing a tool for studying eukaryotic biology. Comprehensive gene dispensability comparisons with budding yeast--the only other eukaryote for which a comprehensive knockout library exists--revealed that 83% of single-copy orthologs in the two yeasts had conserved dispensability. Gene dispensability differed for certain pathways between the two yeasts, including mitochondrial translation and cell cycle checkpoint control. We show that fission yeast has more essential genes than budding yeast and that essential genes are more likely than nonessential genes to be present in a single copy, to be broadly conserved and to contain introns. Growth fitness analyses determined sets of haploinsufficient and haploproficient genes for fission yeast, and comparisons with budding yeast identified specific ribosomal proteins and RNA polymerase subunits, which may act more generally to regulate eukaryotic cell growth.
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The 1,273 essential genes produced a significantly higher proportion of detectable proteins (81.9%, pbinomial < 10−15).
“Moreover, the 1,273 essential genes (Kim et al., 2010) produced a significantly higher proportion of detectable proteins (81.9%, pbinomial < 10−15).”
From Quantitative Analysis of Fission Yeast Transcriptomes and Proteomes in Proliferating and Quiescent Cells (2012) · cites this paper as Kim et al., 2010
Pol II could become limiting, consistent with Pol II subunit mutants being haplo-insufficient and with evidence for transcription factories.
“This low estimate suggests that Pol II could become limiting, consistent with the finding that Pol II subunit mutants are haplo-insufficient (Kim et al., 2010), and with evidence for transcription factories where limiting factors are concentrated for gene expression (Cook, 2010).”
From Quantitative Analysis of Fission Yeast Transcriptomes and Proteomes in Proliferating and Quiescent Cells (2012) · cites this paper as Kim et al., 2010
Hsp90 is an essential, abundant, and ubiquitous eukaryotic chaperone.
“Hsp90 is an essential, abundant, and ubiquitous eukaryotic chaperone that has crucial roles in protein folding and modulates the activities of key regulators.”
From Fungal Hsp90: a biological transistor that tunes cellular outputs to thermal inputs (2012) · cites this paper as [16]
Hsp90 is essential for the growth and viability of evolutionarily divergent yeasts such as S. cerevisiae, S. pombe and C. albicans even under normal growth conditions.
“Indeed, Hsp90 is essential for the growth and viability of evolutionarily divergent yeasts such as S. cerevisiae, Schizosaccharomyces pombe and Candida albicans even under normal growth conditions14-16.”
From Fungal Hsp90: a biological transistor that tunes cellular outputs to thermal inputs (2012) · cites this paper as [16]
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- Authors
- Kim, D.-U., Hayles, J., Kim, D., Wood, V., Park, H.-O., Won, M., Yoo, H.-S., Duhig, T., Nam, M., Palmer, G., Han, S., Jeffery, L., Baek, S.-T., Lee, H., Shim, Y. S., Lee, M., Kim, L., Heo, K.-S., Noh, E. J., Lee, A.-R., Jang, Y.-J., Chung, K.-S., Choi, S.-J., Park, J.-Y., Park, Y., Kim, H. M., Park, S.-K., Park, H.-J., Kang, E.-J., Kim, H. B., Kang, H.-S., Park, H.-M., Kim, K., Song, K., Song, K. B., Nurse, P., & Hoe, K.-L.
- Affiliations
- Korea Advanced Institute of Science and Technology (KR)
- Cancer Research UK (GB)
- Chungnam National University (KR)
- Korea Research Institute of Bioscience and Biotechnology (KR)
- Korea University (KR)
- Daejeon Technopark (KR)
- Seoul National University (KR)
- Kangwon National University (KR)
- Yonsei University (KR)
- Corresponding author
- Hoe, Kwang-Lae
- ORCID
- Venue
- Nature Biotechnology
- Published
- 2010-05-16
- Volume
- 28
- Issue
- 6
- Pages
- 617-623
- ISSN
- 0733-222X (ISSN-L)
- 1087-0156
- 1546-1696
- PMC
- PMC3962850
- OpenAlex
- W2052948442
- Cited by
- 742
- References
- 41
- Citations by year
- 2026: 31 · 2025: 26 · 2024: 41 · 2023: 44 · 2022: 45 · 2021: 40 · 2020: 38 · 2019: 45 · 2018: 40 · 2017: 59 · 2016: 52 · 2015: 48 · 2014: 74 · 2013: 57 · 2012: 49
- FWCI
- 20.60
- Type
- journal article
- Language
- en
- Update notices
- erratum (2010-12-07)
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Nature Biotechnology (publishedVersion)
- PubMed (publishedVersion)
- Europe PMC (PubMed Central) (acceptedVersion)
- PubMed Central (acceptedVersion)
- KRIBB Repository (submittedVersion)
- Open Access System for Information Sharing (Pohang University of Science and Technology) (submittedVersion)
- Funders
- Wellcome Trust (079643, 093917)
- Cancer Research UK
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Bioinformatics and Genomic Networks
- Keywords
- Schizosaccharomyces pombe, gene essentiality, gene dispensability, Saccharomyces cerevisiae, single-copy orthologs, mitochondrial translation, cell cycle checkpoints, haploinsufficiency, ribosomal proteins, RNA polymerase subunits, growth fitness
- MeSH terms
- Diploidy, Genes, Fungal, Haploidy, Saccharomyces cerevisiae, Schizosaccharomyces, Sequence Homology, Nucleic Acid, Species Specificity, Genome, Fungal, Gene Deletion, Genes, Essential
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