The DOI matches its Crossref record; 9 of the 9 references it cites were found in a registry or an index.
DNA damage triggers SAF-A and RNA biogenesis factors exclusion from chromatin coupled to R-loops removal
Open accessAbstract
We previously identified the heterogeneous ribonucleoprotein SAF-A/hnRNP U as a substrate for DNA-PK, a protein kinase involved in DNA damage response (DDR). Using laser micro-irradiation in human cells, we report here that SAF-A exhibits a two-phase dynamics at sites of DNA damage, with a rapid and transient recruitment followed by a prolonged exclusion. SAF-A recruitment corresponds to its binding to Poly(ADP-ribose) while its exclusion is dependent on the activity of ATM, ATR and DNA-PK and reflects the dissociation from chromatin of SAF-A associated with ongoing transcription. Having established that SAF-A RNA-binding domain recapitulates SAF-A dynamics, we show that this domain is part of a complex comprising several mRNA biogenesis proteins of which at least two, FUS/TLS and TAFII68/TAF15, exhibit similar biphasic dynamics at sites of damage. Using an original reporter for live imaging of DNA:RNA hybrids (R-loops), we show a transient transcription-dependent accumulation of R-loops at sites of DNA damage that is prolonged upon inhibition of RNA biogenesis factors exclusion. We propose that a new component of the DDR is an active anti-R-loop mechanism operating at damaged transcribed sites which includes the exclusion of mRNA biogenesis factors such as SAF-A, FUS and TAF15.
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Cites
The DNA Damage Response: Making It Safe to Play with Knives
Ciccia, A., & Elledge, S. J. (2010) · Molecular Cell · doi:10.1016/j.molcel.2010.09.019 · Cited by 4,368
ATM and ATR Substrate Analysis Reveals Extensive Protein Networks Responsive to DNA Damage
Matsuoka, S., Ballif, B. A., Smogorzewska, A., McDonald, E. R., Hurov, K. E., Luo, J., Bakalarski, C. E., Zhao, Z., Solimini, N., Lerenthal, Y., et al. (2007) · Science · doi:10.1126/science.1140321 · Cited by 3,075
ATM-Dependent Chromatin Changes Silence Transcription In cis to DNA Double-Strand Breaks
Shanbhag, N. M., Rafalska-Metcalf, I. U., Balane-Bolivar, C., Janicki, S. M., & Greenberg, R. A. (2010) · Cell · doi:10.1016/j.cell.2010.04.038 · Cited by 737
Chou, D. M., Adamson, B., Dephoure, N. E., Tan, X., Nottke, A. C., Hurov, K. E., Gygi, S. P., Colaiácovo, M. P., & Elledge, S. J. (2010) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1012946107 · Cited by 529
Adamson, B., Smogorzewska, A., Sigoillot, F. D., King, R. W., & Elledge, S. J. (2012) · Nature Cell Biology · doi:10.1038/ncb2426 · Cited by 433
Thompson, L. H. (2012) · Mutation Research/Reviews in Mutation Research · doi:10.1016/j.mrrev.2012.06.002 · Cited by 357
The ATM repair pathway inhibits RNA polymerase I transcription in response to chromosome breaks
Kruhlak, M., Crouch, E. E., Orlov, M., Montaño, C., Gorski, S. A., Nussenzweig, A., Misteli, T., Phair, R. D., & Casellas, R. (2007) · Nature · doi:10.1038/nature05842 · Cited by 305
DNAPKcs-dependent arrest of RNA polymerase II transcription in the presence of DNA breaks
Pankotai, T., Bonhomme, C., Chen, D., & Soutoglou, E. (2012) · Nature Structural & Molecular Biology · doi:10.1038/nsmb.2224 · Cited by 239
Pre-mRNA processing factors meet the DNA damage response
Montecucco, A., & Biamonti, G. (2013) · Frontiers in Genetics · doi:10.3389/fgene.2013.00102 · Cited by 69
Cited by
Liquid demixing of intrinsically disordered proteins is seeded by poly(ADP-ribose)
Altmeyer, M., Neelsen, K. J., Teloni, F., Pozdnyakova, I., Pellegrino, S., Grøfte, M., Rask, M.-B. D., Streicher, W., Jungmichel, S., Nielsen, M. L., et al. (2015) · Nature Communications · doi:10.1038/ncomms9088 · Cited by 587
Cristini, A., Groh, M., Kristiansen, M. S., & Gromak, N. (2018) · Cell Reports · doi:10.1016/j.celrep.2018.04.025 · Cited by 423
Michelini, F., Pitchiaya, S., Vitelli, V., Sharma, S., Gioia, U., Pessina, F., Cabrini, M., Wang, Y., Capozzo, I., Iannelli, F., et al. (2017) · Nature Cell Biology · doi:10.1038/ncb3643 · Cited by 392
The antitumorigenic roles of BRCA1–BARD1 in DNA repair and replication
Tarsounas, M., & Sung, P. (2020) · Nature Reviews Molecular Cell Biology · doi:10.1038/s41580-020-0218-z · Cited by 359
De Magis, A., Manzo, S. G., Russo, M., Marinello, J., Morigi, R., Sordet, O., & Capranico, G. (2019) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1810409116 · Cited by 332
Metadata
- Authors
- Britton, S., Dernoncourt, E., Delteil, C., Froment, C., Schiltz, O., Salles, B., Frit, P., & Calsou, P.
- Venue
- Nucleic Acids Research
- Publisher
- Oxford University Press (OUP)
- Published
- 2014-07-16
- Volume
- 42
- Issue
- 14
- Pages
- 9047-9062
- ISSN
- 0305-1048 (ISSN-L)
- 1362-4962
- OpenAlex
- W2050562740
- Cited by
- 188
- References
- 9
- FWCI
- 5.23
- Type
- journal article
- Language
- en
- Open access
- gold
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- Britton, S., Dernoncourt, E., Delteil, C., Froment, C., Schiltz, O., Salles, B., Frit, P., & Calsou, P. (2014). DNA damage triggers SAF-A and RNA biogenesis factors exclusion from chromatin coupled to R-loops removal. Nucleic Acids Research. https://doi.org/10.1093/nar/gku601
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