The DOI matches its Crossref record; 28 of the 28 references it cites were found in a registry or an index.
Argonaute of the archaeon Pyrococcus furiosus is a DNA-guided nuclease that targets cognate DNA
Open accessAbstract
Functions of prokaryotic Argonautes (pAgo) have long remained elusive. Recently, Argonautes of the bacteria Rhodobacter sphaeroides and Thermus thermophilus were demonstrated to be involved in host defense. The Argonaute of the archaeon Pyrococcus furiosus (PfAgo) belongs to a different branch in the phylogenetic tree, which is most closely related to that of RNA interference-mediating eukaryotic Argonautes. Here we describe a functional and mechanistic characterization of PfAgo. Like the bacterial counterparts, archaeal PfAgo contributes to host defense by interfering with the uptake of plasmid DNA. PfAgo utilizes small 5'-phosphorylated DNA guides to cleave both single stranded and double stranded DNA targets, and does not utilize RNA as guide or target. Thus, with respect to function and specificity, the archaeal PfAgo resembles bacterial Argonautes much more than eukaryotic Argonautes. These findings demonstrate that the role of Argonautes is conserved through the bacterial and archaeal domains of life and suggests that eukaryotic Argonautes are derived from DNA-guided DNA-interfering host defense systems.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Crystal Structure of Argonaute and Its Implications for RISC Slicer Activity
Song, J.-J., Smith, S. K., Hannon, G. J., & Joshua-Tor, L. (2004) · Science · doi:10.1126/science.1102514 · Cited by 1,470
Argonaute proteins: functional insights and emerging roles
Meister, G. (2013) · Nature Reviews Genetics · doi:10.1038/nrg3462 · Cited by 1,030
Fiala, G., & Stetter, K. O. (1986) · Archives of Microbiology · doi:10.1007/bf00413027 · Cited by 909
Structural basis for 5′-nucleotide base-specific recognition of guide RNA by human AGO2
Frank, F., Sonenberg, N., & Nagar, B. (2010) · Nature · doi:10.1038/nature09039 · Cited by 666
Structural basis for 5′-end-specific recognition of guide RNA by the A. fulgidus Piwi protein
Ma, J.-B., Yuan, Y.-R., Meister, G., Pei, Y., Tuschl, T., & Patel, D. J. (2005) · Nature · doi:10.1038/nature03514 · Cited by 657
The RNA-induced Silencing Complex: A Versatile Gene-silencing Machine
Pratt, A. J., & MacRae, I. J. (2009) · Journal of Biological Chemistry · doi:10.1074/jbc.r900012200 · Cited by 602
Structure of an argonaute silencing complex with a seed-containing guide DNA and target RNA duplex
Wang, Y., Juranek, S., Li, H., Sheng, G., Tuschl, T., & Patel, D. J. (2008) · Nature · doi:10.1038/nature07666 · Cited by 577
Origins and evolution of eukaryotic RNA interference
SHABALINA, S., & KOONIN, E. (2008) · Trends in Ecology & Evolution · doi:10.1016/j.tree.2008.06.005 · Cited by 561
Structure of the guide-strand-containing argonaute silencing complex
Wang, Y., Sheng, G., Juranek, S., Tuschl, T., & Patel, D. J. (2008) · Nature · doi:10.1038/nature07315 · Cited by 543
The evolutionary journey of Argonaute proteins
Swarts, D. C., Makarova, K., Wang, Y., Nakanishi, K., Ketting, R. F., Koonin, E. V., Patel, D. J., & van der Oost, J. (2014) · Nature Structural & Molecular Biology · doi:10.1038/nsmb.2879 · Cited by 543
Self-citation
Cited by
Bacterial Argonaute nucleases reveal different modes of DNA targeting in vitro and in vivo
Lisitskaya, L., Kropocheva, E., Agapov, A., Prostova, M., Panteleev, V., Yudin, D., Ryazansky, S., Kuzmenko, A., Aravin, A. A., Esyunina, D., et al. (2023) · Nucleic Acids Research · doi:10.1093/nar/gkad290 · Cited by 26
Zheng, L., Zhou, B., Yang, Y., Zan, B., Zhong, B., Wu, B., Feng, Y., Liu, Q., & Hong, L. (2024) · Chemical Science · doi:10.1039/d3sc06221j · Cited by 11
Metadata
- Authors
- Swarts, D. C., Hegge, J. W., Hinojo, I., Shiimori, M., Ellis, M. A., Dumrongkulraksa, J., Terns, R. M., Terns, M. P., & van der Oost, J.
- Venue
- Nucleic Acids Research
- Publisher
- Oxford University Press (OUP)
- Published
- 2015-04-29
- Volume
- 43
- Issue
- 10
- Pages
- 5120-5129
- ISSN
- 0305-1048 (ISSN-L)
- 1362-4962
- OpenAlex
- W2038482997
- Cited by
- 323
- References
- 28
- FWCI
- 5.66
- Type
- journal article
- Language
- en
- Open access
- gold
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: Argonaute of the archaeon Pyrococcus furiosus is a DNA-guided nuclease that targets cognate DNA. https://app.cochat.ai/papers/10.1093/nar/gkv415
- The paper
- Swarts, D. C., Hegge, J. W., Hinojo, I., Shiimori, M., Ellis, M. A., Dumrongkulraksa, J., Terns, R. M., Terns, M. P., & van der Oost, J. (2015). Argonaute of the archaeon Pyrococcus furiosus is a DNA-guided nuclease that targets cognate DNA. Nucleic Acids Research. https://doi.org/10.1093/nar/gkv415
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
