The DOI matches its Crossref record; 4 of the 4 references it cites were found in a registry or an index.
Assessing genome assembly quality using the LTR Assembly Index (LAI)
Open accessAbstract
Assembling a plant genome is challenging due to the abundance of repetitive sequences, yet no standard is available to evaluate the assembly of repeat space. LTR retrotransposons (LTR-RTs) are the predominant interspersed repeat that is poorly assembled in draft genomes. Here, we propose a reference-free genome metric called LTR Assembly Index (LAI) that evaluates assembly continuity using LTR-RTs. After correcting for LTR-RT amplification dynamics, we show that LAI is independent of genome size, genomic LTR-RT content, and gene space evaluation metrics (i.e., BUSCO and CEGMA). By comparing genomic sequences produced by various sequencing techniques, we reveal the significant gain of assembly continuity by using long-read-based techniques over short-read-based methods. Moreover, LAI can facilitate iterative assembly improvement with assembler selection and identify low-quality genomic regions. To apply LAI, intact LTR-RTs and total LTR-RTs should contribute at least 0.1% and 5% to the genome size, respectively. The LAI program is freely available on GitHub: https://github.com/oushujun/LTR_retriever.
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Cites
BUSCO: assessing genome assembly and annotation completeness with single-copy orthologs
Simão, F. A., Waterhouse, R. M., Ioannidis, P., Kriventseva, E. V., & Zdobnov, E. M. (2015) · Bioinformatics · doi:10.1093/bioinformatics/btv351 · Cited by 13,344
QUAST: quality assessment tool for genome assemblies
Gurevich, A., Saveliev, V., Vyahhi, N., & Tesler, G. (2013) · Bioinformatics · doi:10.1093/bioinformatics/btt086 · Cited by 11,172
The zebrafish reference genome sequence and its relationship to the human genome.
Howe, K., Clark, M. D., Torroja, C. F., Torrance, J., Berthelot, C., Muffato, M., Collins, J. E., Humphray, S., McLaren, K., Matthews, L., et al. (2013) · Nature · doi:10.1038/nature12111 · Cited by 5,100
Assessing the gene space in draft genomes
Parra, G., Bradnam, K., Ning, Z., Keane, T., & Korf, I. (2009) · Nucleic Acids Research · doi:10.1093/nar/gkn916 · Cited by 395 · cited 1×
“5) (19) and Benchmarking Universal Single-Copy Orthologs (BUSCO v3) (2).”
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RepeatModeler2 for automated genomic discovery of transposable element families
Flynn, J. M., Hubley, R., Goubert, C., Rosen, J., Clark, A. G., Feschotte, C., & Smit, A. F. (2020) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1921046117 · Cited by 3,848
De novo assembly, annotation, and comparative analysis of 26 diverse maize genomes
Hufford, M. B., Seetharam, A. S., Woodhouse, M. R., Chougule, K. M., Ou, S., Liu, J., Ricci, W. A., Guo, T., Olson, A., Qiu, Y., et al. (2021) · Science · doi:10.1126/science.abg5289 · Cited by 747
Self-citationPan-genome analysis of 33 genetically diverse rice accessions reveals hidden genomic variations
Qin, P., Lu, H., Du, H., Wang, H., Chen, W., Chen, Z., He, Q., Ou, S., Zhang, H., Li, X., et al. (2021) · Cell · doi:10.1016/j.cell.2021.04.046 · Cited by 578
Self-citationYang, T., Liu, R., Luo, Y., Hu, S., Wang, D., Wang, C., Pandey, M. K., Ge, S., Xu, Q., Li, N., et al. (2022) · Nature Genetics · doi:10.1038/s41588-022-01172-2 · Cited by 191
Standards recommendations for the Earth BioGenome Project
Lawniczak, M. K. N., Durbin, R., Flicek, P., Lindblad-Toh, K., Wei, X., Archibald, J. M., Baker, W. J., Belov, K., Blaxter, M. L., Marques Bonet, T., et al. (2022) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.2115639118 · Cited by 99
Genomic Analyses Unveil Helmeted Guinea Fowl (Numida meleagris) Domestication in West Africa
Shen, Q.-K., Peng, M.-S., Adeola, A. C., Kui, L., Duan, S., Miao, Y.-W., Eltayeb, N. M., Lichoti, J. K., Otecko, N. O., Strillacci, M. G., et al. (2021) · Genome Biology and Evolution · doi:10.1093/gbe/evab090 · Cited by 15
Liu, H.-S., Wang, W.-H., Song, Y.-F., Yang, Y., Zhang, D.-Y., Bai, W.-N., & Zhang, B.-W. (2025) · Current Biology · doi:10.1016/j.cub.2025.09.017 · Cited by 5
Metadata
- Authors
- Ou, S., Chen, J., & Jiang, N.
- Venue
- Nucleic Acids Research
- Publisher
- Oxford University Press (OUP)
- Published
- 2018-08-10
- Volume
- 46
- Issue
- 21
- Pages
- e126-e126
- ISSN
- 0305-1048 (ISSN-L)
- 1362-4962
- OpenAlex
- W2885582175
- Cited by
- 739
- References
- 4
- FWCI
- 9.42
- Type
- journal article
- Language
- en
- Open access
- gold
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