The DOI matches its Crossref record; 20 of the 20 references it cites were found in a registry or an index.
Site-directed, Ligase-Independent Mutagenesis (SLIM): a single-tube methodology approaching 100% efficiency in 4 h
Open accessAbstract
Site-directed, Ligase-Independent Mutagenesis (SLIM) is a novel PCR-mediated mutagenesis approach that can accommodate all three sequence modification types (insertion, deletion and substitution). The method utilizes an inverse PCR amplification of the template by two tailed long primers and two short primers in a single reaction with all steps carried out in one tube. The tailed primers are designed to contain the desired mutation on complementary overhangs at the terminus of PCR products. Upon post-amplification denaturation and re-annealing, heteroduplex formation between the mixed PCR products creates the desired clonable mutated plasmid. The technique is highly robust and suitable for applications in high-throughput gene engineering and library constructions. In this study, SLIM was employed to create sequence insertions, deletion and substitution within bacteriophage T7 gene 5. The overall efficiency for obtaining the desired product was >95%.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Molecular cloning: A laboratory manual
Deininger, P. (1990) · Analytical Biochemistry · doi:10.1016/0003-2697(90)90595-z · Cited by 84,682
Rapid and efficient site-specific mutagenesis without phenotypic selection.
Kunkel, T. A. (1985) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.82.2.488 · Cited by 7,206
Engineering hybrid genes without the use of restriction enzymes: gene splicing by overlap extension
Horton, R. M., Hunt, H. D., Ho, S. N., Pullen, J. K., & Pease, L. R. (1989) · Gene · doi:10.1016/0378-1119(89)90359-4 · Cited by 3,017
High-Resolution Epitope Mapping of hGH-Receptor Interactions by Alanine-Scanning Mutagenesis
Cunningham, B. C., & Wells, J. A. (1989) · Science · doi:10.1126/science.2471267 · Cited by 1,367
The "megaprimer" method of site-directed mutagenesis.
Sarkar, G., & Sommer, S. S. (1990) · PubMed · Cited by 1,165
Site-directed mutagenesis of virtually any plasmid by eliminating a unique site
Deng, W. P., & Nickoloff, J. A. (1992) · Analytical Biochemistry · doi:10.1016/0003-2697(92)90280-k · Cited by 1,060
A general method for rapid site-directed mutagenesis using the polymerase chain reaction
Landt, O., Grunert, H.-P., & Hahn, U. (1990) · Gene · doi:10.1016/0378-1119(90)90351-q · Cited by 635
A simple method for site-directed mutagenesis using the polymerase chain reaction
Hemsley, A., Arnheim, N., Toney, M. D., Cortopassi, G., & Galas, D. J. (1989) · Nucleic Acids Research · doi:10.1093/nar/17.16.6545 · Cited by 612
Site-directed mutagenesis of double-stranded DNA by the polymerase chain reaction
Weiner, M. P., Costa, G. L., Schoettlin, W., Cline, J., Mathur, E., & Bauer, J. C. (1994) · Gene · doi:10.1016/0378-1119(94)90641-6 · Cited by 481
Receptor and Antibody Epitopes in Human Growth Hormone Identified by Homolog-Scanning Mutagenesis
Cunningham, B. C., Jhurani, P., Ng, P., & Wells, J. A. (1989) · Science · doi:10.1126/science.2466339 · Cited by 342
Cited by
Many Families of C. elegans MicroRNAs Are Not Essential for Development or Viability
Alvarez-Saavedra, E., & Horvitz, H. R. (2010) · Current Biology · doi:10.1016/j.cub.2009.12.051 · Cited by 293
Boulias, K., & Horvitz, H. R. (2012) · Cell Metabolism · doi:10.1016/j.cmet.2012.02.014 · Cited by 184
LEDGINs inhibit late stage HIV-1 replication by modulating integrase multimerization in the virions
Desimmie, B. A., Schrijvers, R., Demeulemeester, J., Borrenberghs, D., Weydert, C., Thys, W., Vets, S., Van Remoortel, B., Hofkens, J., De Rijck, J., et al. (2013) · Retrovirology · doi:10.1186/1742-4690-10-57 · Cited by 139
MiR‐205 silences MED1 in hypoxic primary human trophoblasts
Mouillet, J., Chu, T., Nelson, D. M., Mishima, T., & Sadovsky, Y. (2010) · The FASEB Journal · doi:10.1096/fj.09-149724 · Cited by 125
HIV-1 exploits a dynamic multi-aminoacyl-tRNA synthetase complex to enhance viral replication
Duchon, A. A., St. Gelais, C., Titkemeier, N., Hatterschide, J., Wu, L., & Musier-Forsyth, K. (2017) · Journal of Virology · doi:10.1128/jvi.01240-17 · Cited by 48
Metadata
- Authors
- Chiu, J.
- Venue
- Nucleic Acids Research
- Publisher
- Oxford University Press (OUP)
- Published
- 2004-11-29
- Volume
- 32
- Issue
- 21
- Pages
- e174-e174
- ISSN
- 0305-1048 (ISSN-L)
- 1362-4962
- OpenAlex
- W2155596249
- Cited by
- 355
- References
- 20
- FWCI
- 2.73
- Type
- journal article
- Language
- en
- Open access
- bronze
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: Site-directed, Ligase-Independent Mutagenesis (SLIM): a single-tube methodology approaching 100% efficiency in 4 h. https://app.cochat.ai/papers/10.1093/nar/gnh172
- The paper
- Chiu, J. (2004). Site-directed, Ligase-Independent Mutagenesis (SLIM): a single-tube methodology approaching 100% efficiency in 4 h. Nucleic Acids Research. https://doi.org/10.1093/nar/gnh172
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
