MiR‐205 silences MED1 in hypoxic primary human trophoblasts
Open accessAbstract
Acting through degradation of target mRNA or inhibition of translation, microRNAs (miRNAs) regulate development, differentiation, and cellular response to diverse cues. We analyzed changes in miRNA expression in human placental trophoblasts exposed to hypoxia, which may result from hypoperfusion and placental injury. Using an miRNA microarray screen, confirmed by Northern blot analysis, we defined a set of seven miRNAs (miR‐93, miR‐205, miR‐224, miR‐335, miR‐424, miR‐451, and miR‐491) that are differentially regulated in primary trophoblasts exposed to hypoxia. We combined in silico prediction of miRNA targets with gene expression profiling data to identify a series of potential targets for the miRNAs, which were further analyzed using luciferase reporter assays. Among experimentally confirmed targets, we found that the transcriptional coactivator MED1, which plays an important role in placental development, is a target for miR‐205. Using gain‐ and loss‐of‐function assays, we confirmed that miR‐205 interacts with a specific target in the 3′‐UTR sequence of MED1 and silences MED1 expression in human trophoblasts exposed to hypoxia, suggesting that miR‐205 plays a role in trophoblast injury.—Mouillet, J.‐F., Chu, T., Nelson, D. M., Mishima, T., Sadovsky, Y. MiR‐205 silences MED1 in hypoxic primary human trophoblasts. FASEB J. 24, 2030–2039 (2010). www.fasebj.org
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Micro-RNAs identified as being differentially expressed under hypoxia include miR-93, miR-205, miR-224, MiR-335, MiR-424, miR-451, miR-491 and mIR-34a.
“Many of these Other micro-RNAs identified as being differentially expressed under hypoxia include miR-93, miR-205, miR-224, MiR-335, MiR-424, miR-451 and miR-491 (396, 397), and mIR-34a (154), but little is known regarding their functional significance at present.”
From Placental Origins of Chronic Disease (2016) · cites this paper as [396]
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RNA Network Interactions During Differentiation of Human Trophoblasts
Chu, T., Mouillet, J., Cao, Z., Barak, O., Ouyang, Y., & Sadovsky, Y. (2021) · Frontiers in Cell and Developmental Biology · doi:10.3389/fcell.2021.677981 · Cited by 9 · see citing works
Self-citation
- Authors
- Mouillet, J., Chu, T., Nelson, D. M., Mishima, T., & Sadovsky, Y.
- Affiliations
- Magee‐Womens Research Institute Department of Obstetrics, Gynecology, and Reproductive Sciences Pittsburgh Pennsylvania USA
- Magee-Womens Research Institute (US)
- Washington University in St. Louis (US)
- Corresponding author
- Sadovsky, Yoel
- Venue
- The FASEB Journal
- Publisher
- Wiley
- Published
- 2010-01-11
- Volume
- 24
- Issue
- 6
- Pages
- 2030-2039
- ISSN
- 0892-6638 (ISSN-L)
- 1530-6860
- PMC
- PMC2874470
- OpenAlex
- W2115901073
- Cited by
- 125
- References
- 96
- Citations by year
- 2026: 4 · 2025: 1 · 2024: 2 · 2023: 6 · 2022: 6 · 2021: 11 · 2020: 10 · 2019: 5 · 2018: 9 · 2017: 7 · 2016: 6 · 2015: 11 · 2014: 6 · 2013: 15 · 2012: 7
- FWCI
- 17.53
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- The FASEB Journal (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institutes of Health (R01‐HD29190, R21‐HD053878, R01‐HD45675)
- Topics
- Health Sciences › Medicine › Obstetrics and Gynecology › Pregnancy and preeclampsia studies
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › MicroRNA in disease regulation
- Life Sciences › Immunology and Microbiology › Immunology › Reproductive System and Pregnancy
- Keywords
- miR-205, MED1, microRNA, hypoxia, trophoblast, placental development, placental damage, microRNA target regulation, 3' untranslated region, miRNA microarray, miR-451, miR-424, miR-335, miR-93, miR-224, placental hypoperfusion
- MeSH terms
- Hypoxia, Female, Humans, Luciferases, Placenta, Pregnancy, RNA, Messenger, Trophoblasts, Blotting, Northern, Blotting, Western, In Situ Hybridization, 3' Untranslated Regions, Gene Expression Profiling, MicroRNAs, Mediator Complex Subunit 1
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