Cited by 125Pregnancy and preeclampsia studiesMicroRNA in disease regulationReproductive System and Pregnancy

MiR‐205 silences MED1 in hypoxic primary human trophoblasts

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Mouillet, Jean‐Francois, Chu, Tianjiao, Nelson, D. Michael, Mishima, Takuya, Sadovsky, Yoel·2010·The FASEB Journal

Abstract

Acting through degradation of target mRNA or inhibition of translation, microRNAs (miRNAs) regulate development, differentiation, and cellular response to diverse cues. We analyzed changes in miRNA expression in human placental trophoblasts exposed to hypoxia, which may result from hypoperfusion and placental injury. Using an miRNA microarray screen, confirmed by Northern blot analysis, we defined a set of seven miRNAs (miR‐93, miR‐205, miR‐224, miR‐335, miR‐424, miR‐451, and miR‐491) that are differentially regulated in primary trophoblasts exposed to hypoxia. We combined in silico prediction of miRNA targets with gene expression profiling data to identify a series of potential targets for the miRNAs, which were further analyzed using luciferase reporter assays. Among experimentally confirmed targets, we found that the transcriptional coactivator MED1, which plays an important role in placental development, is a target for miR‐205. Using gain‐ and loss‐of‐function assays, we confirmed that miR‐205 interacts with a specific target in the 3′‐UTR sequence of MED1 and silences MED1 expression in human trophoblasts exposed to hypoxia, suggesting that miR‐205 plays a role in trophoblast injury.—Mouillet, J.‐F., Chu, T., Nelson, D. M., Mishima, T., Sadovsky, Y. MiR‐205 silences MED1 in hypoxic primary human trophoblasts. FASEB J. 24, 2030–2039 (2010). www.fasebj.org

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  • Micro-RNAs identified as being differentially expressed under hypoxia include miR-93, miR-205, miR-224, MiR-335, MiR-424, miR-451, miR-491 and mIR-34a.

    “Many of these Other micro-RNAs identified as being differentially expressed under hypoxia include miR-93, miR-205, miR-224, MiR-335, MiR-424, miR-451 and miR-491 (396, 397), and mIR-34a (154), but little is known regarding their functional significance at present.”

    From Placental Origins of Chronic Disease (2016) · cites this paper as [396]

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Authors
Mouillet, J., Chu, T., Nelson, D. M., Mishima, T., & Sadovsky, Y.
Affiliations
  • Magee‐Womens Research Institute Department of Obstetrics, Gynecology, and Reproductive Sciences Pittsburgh Pennsylvania USA
  • Magee-Womens Research Institute (US)
  • Washington University in St. Louis (US)
Corresponding author
Sadovsky, Yoel
Venue
The FASEB Journal
Publisher
Wiley
Published
2010-01-11
Volume
24
Issue
6
Pages
2030-2039
ISSN
  • 0892-6638 (ISSN-L)
  • 1530-6860
OpenAlex
W2115901073
Cited by
125
References
96
Citations by year
2026: 4 · 2025: 1 · 2024: 2 · 2023: 6 · 2022: 6 · 2021: 11 · 2020: 10 · 2019: 5 · 2018: 9 · 2017: 7 · 2016: 6 · 2015: 11 · 2014: 6 · 2013: 15 · 2012: 7
FWCI
17.53
Type
journal article
Language
en
Open access
Locations
  • The FASEB Journal (publishedVersion)
  • Europe PMC (PubMed Central) (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Funders
National Institutes of Health (R01‐HD29190, R21‐HD053878, R01‐HD45675)
Topics
  • Health Sciences › Medicine › Obstetrics and Gynecology › Pregnancy and preeclampsia studies
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › MicroRNA in disease regulation
  • Life Sciences › Immunology and Microbiology › Immunology › Reproductive System and Pregnancy
Keywords
miR-205, MED1, microRNA, hypoxia, trophoblast, placental development, placental damage, microRNA target regulation, 3' untranslated region, miRNA microarray, miR-451, miR-424, miR-335, miR-93, miR-224, placental hypoperfusion
MeSH terms
Hypoxia, Female, Humans, Luciferases, Placenta, Pregnancy, RNA, Messenger, Trophoblasts, Blotting, Northern, Blotting, Western, In Situ Hybridization, 3' Untranslated Regions, Gene Expression Profiling, MicroRNAs, Mediator Complex Subunit 1

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