Single-cell transcriptomic landscape of human blood cells
Open accessAbstract
High throughput single-cell RNA-seq has been successfully implemented to dissect the cellular and molecular features underlying hematopoiesis. However, an elaborate and comprehensive transcriptome reference of the whole blood system is lacking. Here, we profiled the transcriptomes of 7551 human blood cells representing 32 immunophenotypic cell types, including hematopoietic stem cells, progenitors and mature blood cells derived from 21 healthy donors. With high sequencing depth and coverage, we constructed a single-cell transcriptional atlas of blood cells (ABC) on the basis of both protein-coding genes and long noncoding RNAs (lncRNAs), and showed a high consistence between them. Notably, putative lncRNAs and transcription factors regulating hematopoietic cell differentiation were identified. While common transcription factor regulatory networks were activated in neutrophils and monocytes, lymphoid cells dramatically changed their regulatory networks during differentiation. Furthermore, we showed a subset of nucleated erythrocytes actively expressing immune signals, suggesting the existence of erythroid precursors with immune functions. Finally, a web portal offering transcriptome browsing and blood cell type prediction has been established. Thus, our work provides a transcriptional map of human blood cells at single-cell resolution, thereby offering a comprehensive reference for the exploration of physiological and pathological hematopoiesis.
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- Authors
- Xie, X., Liu, M., Zhang, Y., Wang, B., Zhu, C., Wang, C., Li, Q., Huo, Y., Guo, J., Xu, C., Hu, L., Pang, A., Ma, S., Wang, L., Cao, W., Chen, S., Li, Q., Zhang, S., Zhao, X., Zhou, W., Luo, H., Zheng, G., Jiang, E., Feng, S., Chen, L., Shi, L., Cheng, H., Hao, S., Zhu, P., & Cheng, T.
- Affiliations
- Chinese Academy of Medical Sciences & Peking Union Medical College (CN)
- Central South University (CN)
- State Key Laboratory of Experimental Hematology and National Clinical Research Center for Blood Diseases, Institute of Hematology and Blood Diseases Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin 300020, China
- Zhengzhou University (CN)
- Corresponding author
- Hao, Sha
- Zhu, Ping
- Cheng, Tao
- ORCID
- Xie, Xiaowei
- Liu, Mengyao
- Zhang, Yawen
- Wang, Bingrui
- Zhu, Caiying
- Li, Qing
- Huo, Yingying
- Guo, Jiaojiao
- Hu, Linping
- Pang, Aiming
- Ma, Shihui
- Wang, Lina
- Cao, Wenbin
- Chen, Shulian
- Li, Qiuling
- Zhao, Xueying
- Zhou, Wen
- Luo, Hongbo
- Zheng, Guoguang
- Jiang, Erlie
- Feng, Sizhou
- Chen, Lixiang
- Shi, Lihong
- Cheng, Hui
- Hao, Sha
- Zhu, Ping
- Cheng, Tao
- Venue
- National Science Review
- Publisher
- Oxford University Press (OUP)
- Published
- 2020-08-24
- Volume
- 8
- Issue
- 3
- Pages
- nwaa180-nwaa180
- ISSN
- 2053-714X (ISSN-L)
- 2095-5138
- PMC
- PMC8288407
- OpenAlex
- W3080202759
- Cited by
- 145
- References
- 32
- Citations by year
- 2026: 17 · 2025: 26 · 2024: 22 · 2023: 33 · 2022: 31 · 2021: 15 · 2020: 1
- FWCI
- 6.49
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- Locations
- National Science Review (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Funders
- Ministry of Science and Technology (2018YFA0107801)
- National Key Research and Development Program of China (2016YFA0100600)
- National Natural Science Foundation of China (2017YFA0103400)
- CAMS (2018YFA0107804)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Single-cell and spatial transcriptomics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Phylogenetic Studies
- Keywords
- human blood cells, hematopoiesis, single-cell transcriptome, hematopoietic stem cells, hematopoietic progenitor cells, long non-coding RNA, transcriptional regulatory networks, hematopoietic differentiation, neutrophils, monocytes, lymphoid cells, erythroid precursors, nucleated erythrocytes, transcriptome atlas
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