The DOI matches its Crossref record; 38 of the 38 references it cites were found in a registry or an index.
Free Radical-Independent Protection by Nerve Growth Factor and Bcl-2 of PC12 Cells from Hydrogen Peroxide-Triggered Apoptosis
Abstract
To investigate the mechanism of oxidative stress induced death of PC12 cells, we performed confocal and flow cytometric analysis with a reactive oxygen species (ROS)-specific fluorogen, 6-carboxy-2',7'-dichlorodihydrofluorescein diacetate, di(acetoxymethyl ester) (C-DCDHF-DA). Hydrogen peroxide significantly decreased the number of viable PC12 cells after 24 h. Hydrogen peroxide caused membrane blebbing, nuclear condensation and DNA fragmentation, indicating that the PC12 cells died due to apoptosis. The hydrogen peroxide-triggered apoptosis of PC12 cells was associated with enhanced ROS production in a dose-dependent manner by measuring with C-DCDHF-DA. Nerve growth factor (NGF) and Bcl-2 inhibited the hydrogen peroxide-induced apoptosis of PC12 cells. Neither of them, however, reduced the ROS production in PC12 cells. These data suggest that NGF or Bcl-2 protects PC12 cells from hydrogen peroxide-triggered apoptosis independently from ROS production.
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“| Ethanolic root extract | Parkinsonism | Mice | Increase in striatum dopamine | [306] |”
From Withania somnifera (L.) Dunal (Ashwagandha): A comprehensive review on ethnopharmacology, pharmacotherapeutics, biomedicinal and toxicological aspects (2021) · cites this paper as [306]
In the citing paper
“| Withaferin A | 3-NP induced HD | Rats | Increase in free radical scavenging activity | [53,306] |”
From Withania somnifera (L.) Dunal (Ashwagandha): A comprehensive review on ethnopharmacology, pharmacotherapeutics, biomedicinal and toxicological aspects (2021) · cites this paper as [306]
Citations
Cites
Glucocorticoid-induced thymocyte apoptosis is associated with endogenous endonuclease activation
Wyllie, A. H. (1980) · Nature · doi:10.1038/284555a0 · Cited by 4,499
Schreck, R., RIEBER, P., & Baeuerle, P. A. (1991) · The EMBO Journal · doi:10.1002/j.1460-2075.1991.tb07761.x · Cited by 3,793
Bcl-2 functions in an antioxidant pathway to prevent apoptosis
Hockenbery, D. M., Oltvai, Z. N., Yin, X., Milliman, C. L., & Korsmeyer, S. J. (1993) · Cell · doi:10.1016/0092-8674(93)80066-n · Cited by 3,402
Cell death during development of the nervous system
Oppenheim, R. W. (1991) · Annual Review of Neuroscience · doi:10.1146/annurev.ne.14.030191.002321 · Cited by 3,084
Ji, Y. (1993) · Cell · doi:10.1016/0092-8674(93)90485-9 · Cited by 2,497
Genetic control of programmed cell death in the nematode C. elegans
Ellis, H. (1986) · Cell · doi:10.1016/0092-8674(86)90004-8 · Cited by 1,828
Developmental cell death: morphological diversity and multiple mechanisms
Clarke, P. H. (1990) · Anatomy and Embryology · doi:10.1007/bf00174615 · Cited by 1,695
Bcl-2 inhibition of neural death: decreased generation of reactive oxygen species
Kane, D. J., Sarafian, T. A., Anton, R., Hahn, H., Gralla, E. B., Valentine, J. S., Örd, T., & Bredesen, D. E. (1993) · Science · doi:10.1126/science.8235659 · Cited by 1,671
Redox Regulation of Fos and Jun DNA-Binding Activity in Vitro
Abate, C., Patel, L., Rauscher, F. J., & Curran, T. (1990) · Science · doi:10.1126/science.2118682 · Cited by 1,573
Hengartner, M. O., & Horvitz, H. R. (1994) · Cell · doi:10.1016/0092-8674(94)90506-1 · Cited by 1,167
THE NERVE GROWTH FACTOR: PURIFICATION AS A 30,000-MOLECULAR-WEIGHT PROTEIN
Bocchini, V., & Angeletti, P. U. (1969) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.64.2.787 · Cited by 910
Reynolds, I., & Hastings, T. (1995) · The Journal of Neuroscience · doi:10.1523/jneurosci.15-05-03318.1995 · Cited by 777
Greenlund, L. J. S., Deckwerth, T. L., & Johnson, E. M. (1995) · Neuron · doi:10.1016/0896-6273(95)90287-2 · Cited by 738
Programmed cell death and Bcl-2 protection in very low oxygen
Jacobson, M. D., & RAFF, M. C. (1995) · Nature · doi:10.1038/374814a0 · Cited by 625
bcl-2 inhibits death of central neural cells induced by multiple agents.
Zhong, L. T., Sarafian, T. A., Kane, D. J., Charles, A. C., Mah, S. P., Edwards, R. H., & Bredesen, D. E. (1993) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.90.10.4533 · Cited by 619
Mattson, M. P., Lovell, M. A., Furukawa, K., & Markesbery, W. R. (1995) · Journal of Neurochemistry · doi:10.1046/j.1471-4159.1995.65041740.x · Cited by 615
Prevention of hypoxia-induced cell death by Bcl-2 and Bcl-xL
Shimizu, S., Eguchi, Y., Kosaka, H., Kamiike, W., Matsuda, H., & Tsujimoto, Y. (1995) · Nature · doi:10.1038/374811a0 · Cited by 611
Rapid Communication: Oxidative Stress Induces Apoptosis in Embryonic Cortical Neurons
Ratan, R. R., Murphy, T. H., & Baraban, J. M. (1994) · Journal of Neurochemistry · doi:10.1046/j.1471-4159.1994.62010376.x · Cited by 585
Greene, L. (1978) · The Journal of Cell Biology · doi:10.1083/jcb.78.3.747 · Cited by 432
Batistatou, A. K., & Greene, L. A. (1991) · The Journal of Cell Biology · doi:10.1083/jcb.115.2.461 · Cited by 357
Rukenstein, A., Rydel, R. E., & Greene, L. (1991) · Journal of Neuroscience · doi:10.1523/jneurosci.11-08-02552.1991 · Cited by 337
Down-regulation of copper/zinc superoxide dismutase causes apoptotic death in PC12 neuronal cells.
Troy, C. M., & Shelanski, M. L. (1994) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.91.14.6384 · Cited by 271
A system for characterizing cellular and molecular events in programmed neuronal cell death
Pittman, R. N., Wang, S., DiBenedetto, A. J., & Mills, J. C. (1993) · Journal of Neuroscience · doi:10.1523/jneurosci.13-09-03669.1993 · Cited by 265
Suda, K., Barde, Y., & Thoenen, H. (1978) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.75.8.4042 · Cited by 245
Walkinshaw, G., & Waters, C. M. (1995) · Journal of Clinical Investigation · doi:10.1172/jci117946 · Cited by 242
Exposure to hydrogen peroxide induces cell death via apoptosis in cultured rat cortical neurons
Whittemore, E. R., Loo, D. T., & Cotman, C. W. (1994) · Neuroreport · doi:10.1097/00001756-199407000-00019 · Cited by 205
Sgonc, R., Boeck, G., Dietrich, H., Gruber, J., Recheis, H., & Wick, G. (1994) · Trends in Genetics · doi:10.1016/0168-9525(94)90140-6 · Cited by 184
Jackson, G. R., Apffel, L., Werrbach‐Perez, K., & Perez-Polo, J. R. (1990) · Journal of Neuroscience Research · doi:10.1002/jnr.490250313 · Cited by 159
Pognonec, P., Kato, H., & Roeder, R. G. (1992) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)35801-0 · Cited by 127
Satoh, T., Sakai, N., Enokido, Y., Uchiyama, Y., & Hatanaka, H. (1996) · Brain Research · doi:10.1016/0006-8993(96)00527-6 · Cited by 114
Self-citationSatoh, T. (1996) · Brain Research · doi:10.1016/s0006-8993(96)00527-6 · Cited by 102
Self-citationInhibition of Drug‐Induced Apoptosis by Survival Factors in PC12 Cells
Lindenboim, L., Haviv, R., & Stein, R. (1995) · Journal of Neurochemistry · doi:10.1046/j.1471-4159.1995.64031054.x · Cited by 78
Apoptotic cell death occurs in hippocampal neurons cultured in a high oxygen atmosphere
Enokido, Y., & Hatanaka, H. (1993) · Neuroscience · doi:10.1016/0306-4522(93)90041-d · Cited by 74
Self-citationJoseph, R., Li, W., & Han, E. (1993) · Molecular Brain Research · doi:10.1016/0169-328x(93)90074-y · Cited by 58
Neuronal differentiation of PC12 cells as a result of prevention of cell death by bcl‐2
Sato, N., Hotta, K., Waguri, S., Nitatori, T., Tohyama, K., Tsujimoto, Y., & Uchiyama, Y. (1994) · Journal of Neurobiology · doi:10.1002/neu.480251005 · Cited by 55
Self-citationOxygen-induced apoptosis in PC12 cells with special reference to the role of Bcl-2
Kubo, T., Enokido, Y., Yamada, M., Oka, T., Uchiyama, Y., & Hatanaka, H. (1996) · Brain Research · doi:10.1016/0006-8993(96)00542-2 · Cited by 32
Self-citationEnokido, Y., & Hatanaka, H. (1990) · Brain Research · doi:10.1016/0006-8993(90)90004-u · Cited by 31
Self-citationSatoh, T., Sakai, N., Kubo, T., Enokido, Y., Uchiyama, Y., & Hatanaka, H. (1995) · Neuroscience Letters · doi:10.1016/0304-3940(95)12150-1 · Cited by 15
Self-citation
Cited by
Sorted by citations
Reactive oxygen intermediates as mediators of programmed cell death in plants and animals
Jabs, T. (1999) · Biochemical Pharmacology · doi:10.1016/s0006-2952(98)00227-5 · Cited by 600
Zhang, P., Liu, B., Kang, S. W., Seo, M. S., Rhee, S. G., & Obeid, L. M. (1997) · Journal of Biological Chemistry · doi:10.1074/jbc.272.49.30615 · Cited by 370
Paul, S., Chakraborty, S., Anand, U., Dey, S., Nandy, S., Ghorai, M., Saha, S. C., Patil, M. T., Kandimalla, R., Proćków, J., et al. (2021) · Biomedicine & Pharmacotherapy · doi:10.1016/j.biopha.2021.112175 · Cited by 306
Mechanisms underlying hypoxia-induced neuronal apoptosis
Banasiak, K. J., Xia, Y., & Haddad, G. G. (2000) · Progress in Neurobiology · doi:10.1016/s0301-0082(00)00011-3 · Cited by 306
Zhang, L., Yu, H., Sun, Y., Lin, X., Chen, B., Tan, C., Cao, G.-X., & Wang, Z. (2007) · European Journal of Pharmacology · doi:10.1016/j.ejphar.2007.01.089 · Cited by 262
Metadata
- Authors
- Satoh, T., Sakai, N., Enokido, Y., Uchiyama, Y., & Hatanaka, H.
- Affiliations
- The University of Osaka (JP)
- Institute for Protein Research, The University of Osaka (JP)
- Corresponding author
- Hatanaka, H.
- Venue
- Journal of Biochemistry
- Publisher
- Oxford University Press (OUP)
- Published
- 1996-09-01
- Volume
- 120
- Issue
- 3
- Pages
- 540-546
- ISSN
- 0021-924X (ISSN-L)
- OpenAlex
- W2023797581
- Cited by
- 146
- References
- 37
- Citations by year
- 2025: 1 · 2023: 6 · 2022: 3 · 2021: 2 · 2020: 5 · 2018: 4 · 2017: 5 · 2016: 3 · 2015: 5 · 2014: 4 · 2013: 7 · 2012: 6
- FWCI
- 2.27
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- The Journal of Biochemistry (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Cell death mechanisms and regulation
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Redox biology and oxidative stress
- Life Sciences › Neuroscience › Neurology › Neuroprotection in Cerebral Ischemia
- Keywords
- nerve growth factor, Bcl-2, PC12 cells, hydrogen peroxide, apoptosis, oxidative stress, reactive oxygen species, DNA fragmentation, membrane blebbing, neuroprotection
- MeSH terms
- Animals, Cell Membrane, Cell Survival, Flow Cytometry, Free Radicals, Hydrogen Peroxide, Kinetics, Nerve Growth Factors, Recombinant Proteins, Transfection, PC12 Cells, Apoptosis, Reactive Oxygen Species, Oxidative Stress, Microscopy, Confocal, Proto-Oncogene Proteins c-bcl-2, Rats
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