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Cited by 706

The DOI matches its Crossref record; 15 of the 15 references it cites were found in a registry or an index.

Pore Size of Porous Hydroxyapatite as the Cell-Substratum Controls BMP-Induced Osteogenesis

Tsuruga, E., Takita, H., Itoh, H., Wakisaka, Y., Kuboki, Y.·1997·Journal of Biochemistry
DOI 10.1093/oxfordjournals.jbchem.a021589
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Abstract

To elucidate the biochemical mechanism of osteogenesis, the effect of matrix geometry upon the osteogenesis induced by bone morphogenetic protein (BMP) was studied. A series of five porous hydroxyapatites with different pore sizes, 106-212, 212-300, 300-400, 400-500, and 500-600 microns, was prepared. A block (approximately 5 x 5 x 1 mm, 40.0 mg) of each hydroxyapatite ceramics was combined with 4 micrograms of recombinant human BMP-2 and implanted subcutaneously into the back skin of rat. Osteoinductive ability of each implant was estimated by quantifying osteocalcin content and alkaline phosphatase activity in the implant up to 4 wk after implantation. In the ceramics of 106-212 microns, the highest alkaline phosphatase activity was found 2 wk after implantation, and the highest osteocalcin content 4 wk after implantation, consistent with the results observed with particulate porous hydroxyapatite [Kuboki, Y. et al. (1995) Connect. Tissue Res. 32: 219-226]. Comparison of the alkaline phosphatase activities at 2 wk and the osteocalcin contents at 4 wk after implantation revealed that the highest amount of bone was produced in the ceramics implants with pore size of 300-400 microns. In the ceramics with smaller or larger pore sizes, the amount of bone formation decreased as the pore size deviated from 300-400 microns. The results indicated that the optimal pore size for attachment, differentiation and growth of osteoblasts and vascularization is approximately 300-400 microns. This study using chemically identical but geometrically different cell substrata is the first demonstration that a matrix with a certain geometrical size is most favorable for cell differentiation.

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Metadata

Authors
Tsuruga, E., Takita, H., Itoh, H., Wakisaka, Y., & Kuboki, Y.
Venue
Journal of Biochemistry
Publisher
Oxford University Press (OUP)
Published
1997-02-01
Volume
121
Issue
2
Pages
317-324
ISSN
0021-924X (ISSN-L)
DOI
10.1093/oxfordjournals.jbchem.a021589
OpenAlex
W1972211130
Cited by
706
References
15
FWCI
8.10
Type
journal article
Language
en
Open access
closed

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CoChat. (2026). Citation check: Pore Size of Porous Hydroxyapatite as the Cell-Substratum Controls BMP-Induced Osteogenesis. https://app.cochat.ai/papers/10.1093/oxfordjournals.jbchem.a021589
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Tsuruga, E., Takita, H., Itoh, H., Wakisaka, Y., & Kuboki, Y. (1997). Pore Size of Porous Hydroxyapatite as the Cell-Substratum Controls BMP-Induced Osteogenesis. Journal of Biochemistry. https://doi.org/10.1093/oxfordjournals.jbchem.a021589

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Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.