Expression of Genes for Photosynthesis and the Relationship to Salt Tolerance of Alfalfa (Medicago sativa) Cells
Abstract
The basis for the salt tolerant phenotype of a line of Medicago sativa (alfalfa) cells (HG2-N1) derived by selection from a salt sensitive line (HG2) was studied. The salt tolerant HG2-N1 cell line shows eleven fold elevated chlorophyll content over that of the parent salt sensitive HG2 cell line, with an additional two fold increase in chlorophyll levels when the cells are grown in 1% NaCl. In this study, we demonstrate that the chlorophyll accumulation and response to salt was associated with large increases in the two photosynthesis related mRNAs, rbcL (ribulose-l,5-bis-phosphate carboxylase [Rubisco] large subunit) and rbcS (Rubisco small subunit) and a substantial increase in the activity of the holoenzyme. The salinity-induced increase in catalytically competent Rubisco protein in the salt tolerant cell line was highly responsive to light and correlated with the salt tolerant phenotype. In addition, NaCl stimulated rbcL and rbcS mRNA and Rubisco accumulation in dark grown salt tolerant cells, indicating that salt could substitute to some degree for light in stimulating increases in specific mRNA and protein concentrations. Increased photosynthetic competence associated with these increased protein levels was apparently important in contributing to the salt tolerant phenotype of HG2-N1, since PS II electron transport inhibitors (DCMU, cyanazine) were found to significantly reduce the growth of this cell line in the presence of salt, but not in the absence of salt. These…
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- Authors
- Winicov, I., & Seemann, J. R.
- Affiliations
- University of Nevada, Reno (US)
- Corresponding author
- Ilga Winicov
- ORCID
- Jeffrey R. Seemann
- Venue
- Plant and Cell Physiology
- Publisher
- Oxford University Press (OUP)
- Published
- 1990
- ISSN
- 0032-0781 (ISSN-L)
- 1471-9053
- OpenAlex
- W1869599190
- Cited by
- 46
- References
- 0
- Citations by year
- 2025: 1 · 2022: 1 · 2021: 1 · 2020: 2 · 2019: 1 · 2018: 1 · 2016: 1 · 2015: 1 · 2014: 1 · 2013: 2 · 2012: 1
- FWCI
- 2.12
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Plant and Cell Physiology (publishedVersion)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Stress Responses and Tolerance
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Photosynthetic Processes and Mechanisms
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant nutrient uptake and metabolism
- Keywords
- salt tolerance, alfalfa, photosynthesis, Rubisco, chlorophyll accumulation, photosystem II electron transport, DCMU, cyanazine, light response, sodium chloride
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