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Identification of Plant Cytokinin Biosynthetic Enzymes as Dimethylallyl Diphosphate:ATP/ADP Isopentenyltransferases
Abstract
It has been believed that the key step in cytokinin biosynthesis is the addition of a 5-carbon chain to the N(6) of AMP. To identify cytokinin biosynthesis enzymes that catalyze the formation of the isopentenyl side chain of cytokinins, the Arabidopsis genomic sequence was searched for genes that could code for isopentenyltransferases. This resulted in the identification of nine putative genes for isopentenyltransferases. One of these, AtIPT4, was subjected to detailed analysis. Overexpression of AtIPT4 caused cytokinin-independent shoot formation on calli. As shoot formation on calli normally occurs only when cytokinins are applied, it suggested that this gene product catalyzed cytokinin biosynthesis in plants. Recombinant AtIPT4 catalyzed the transfer of an isopentenyl group from dimethylallyl diphosphate to the N(6) of ATP and ADP, but not to that of AMP. AtIPT4 did not exhibit the DMAPP:tRNA isopentenyltransferase activity. These results indicate that cytokinins are, at least in part, synthesized from ATP and ADP in plants.
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Citations
Cited by
Nordström, A., Tarkowski, P., Tarkowska, D., Norbaek, R., Åstot, C., Dolezal, K., & Sandberg, G. (2004) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0402504101 · Cited by 574
Auxin controls local cytokinin biosynthesis in the nodal stem in apical dominance
Tanaka, M., Takei, K., Kojima, M., Sakakibara, H., & Mori, H. (2006) · The Plant Journal · doi:10.1111/j.1365-313x.2006.02656.x · Cited by 512
Auxin-cytokinin interactions in the control of shoot branching
Shimizu-Sato, S., Tanaka, M., & Mori, H. (2009) · Plant Molecular Biology · doi:10.1007/s11103-008-9416-3 · Cited by 387
Cytokinins. New insights into a classic phytohormone
Haberer, G., & Kieber, J. J. (2002) · Plant Physiology · doi:10.1104/pp.010773 · Cited by 334
Metadata
- Authors
- Kakimoto, T.
- Venue
- Plant and Cell Physiology
- Publisher
- Oxford University Press (OUP)
- Published
- 2001-07-15
- Volume
- 42
- Issue
- 7
- Pages
- 677-685
- ISSN
- 0032-0781 (ISSN-L)
- 1471-9053
- OpenAlex
- W2122612270
- Cited by
- 496
- References
- 0
- FWCI
- 27.37
- Type
- journal article
- Language
- en
- Open access
- closed
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- CoChat. (2026). Citation check: Identification of Plant Cytokinin Biosynthetic Enzymes as Dimethylallyl Diphosphate:ATP/ADP Isopentenyltransferases. https://app.cochat.ai/papers/10.1093/pcp/pce112
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- Kakimoto, T. (2001). Identification of Plant Cytokinin Biosynthetic Enzymes as Dimethylallyl Diphosphate:ATP/ADP Isopentenyltransferases. Plant and Cell Physiology. https://doi.org/10.1093/pcp/pce112
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