Auxin controls local cytokinin biosynthesis in the nodal stem in apical dominance
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Summary: In intact plants, the shoot apex grows predominantly and inhibits outgrowth of axillary buds. After decapitation of the shoot apex, outgrowth of axillary buds begins. This phenomenon is called an apical dominance. Although the involvement of auxin, which represses outgrowth of axillary buds, and cytokinin (CK), which promotes outgrowth of axillary buds, has been proposed, little is known about the underlying molecular mechanisms. In the present study, we demonstrated that auxin negatively regulates local CK biosynthesis in the nodal stem by controlling the expression level of the pea (Pisum sativumL.) gene adenosine phosphate–isopentenyltransferase (PsIPT), which encodes a key enzyme in CK biosynthesis. Before decapitation, PsIPT1 and PsIPT2 transcripts were undetectable; after decapitation, they were markedly induced in the nodal stem along with accumulation of CK. Expression of PsIPT was repressed by the application of indole‐3‐acetic acid (IAA). In excised nodal stem, PsIPT expression and CK levels also increased under IAA‐free conditions. Furthermore, β‐glucuronidase expression, under the control of the PsIPT2 promoter region in transgenic Arabidopsis, was repressed by an IAA. Our results indicate that in apical dominance one role of auxin is to repress local biosynthesis of CK in the nodal stem and that, after decapitation, CKs, which are thought to be derived from the roots, are locally biosynthesized in the nodal stem rather than in the roots.
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Basipetally transported auxin from the plant apex decreases expression of the cytokinin biosynthesis gene IPT in the stem.
“Basipetally transported auxin from the plant apex decreases expression of the cytokinin biosynthesis gene ISOPENTENYLTRANSFERASE (IPT) in the stem (Tanaka et al., 2006).”
From Shaping plant architecture (2015) · cites this paper as Tanaka et al., 2006
In pea, PsIPT1 and PsIPT2 genes are induced in the nodal stem near the axillary buds after decapitation, and increased cytokinin levels could be detected in excised nodal stems.
“In pea, the PsIPT1 and PsIPT2 genes are induced in the nodal stem near the axillary buds after decapitation. Consistently, increased cytokinin levels could be detected in excised nodal stems (Tanaka et al., 2006).”
From Shaping plant architecture (2015) · cites this paper as Tanaka et al., 2006
Cites
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“Basipetally transported auxin from the plant apex decreases expression of the cytokinin biosynthesis gene ISOPENTENYLTRANSFERASE (IPT) in the stem (Tanaka et al., 2006).”
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- Authors
- Tanaka, M., Takei, K., Kojima, M., Sakakibara, H., & Mori, H.
- Affiliations
- Nagoya University (JP)
- RIKEN (JP)
- Corresponding author
- Mori, Hitoshi
- Venue
- The Plant Journal
- Publisher
- Wiley
- Published
- 2006-02-17
- Volume
- 45
- Issue
- 6
- Pages
- 1028-1036
- ISSN
- 0960-7412 (ISSN-L)
- 1365-313X
- OpenAlex
- W1978143022
- Cited by
- 512
- References
- 9
- Citations by year
- 2026: 9 · 2025: 23 · 2024: 23 · 2023: 19 · 2022: 33 · 2021: 29 · 2020: 36 · 2019: 22 · 2018: 35 · 2017: 29 · 2016: 24 · 2015: 27 · 2014: 18 · 2013: 37 · 2012: 27
- FWCI
- 27.89
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- The Plant Journal (publishedVersion)
- PubMed (publishedVersion)
- Institutional Repositories DataBase (IRDB) (submittedVersion, cc-by-nc-nd)
- Nagoya University Repository (NAGOYA Repository) (submittedVersion, cc-by-nc-nd)
- Funders
- Shimane University
- Japan Society for the Promotion of Science (17051014, 17027013)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Molecular Biology Research
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Parasitism and Resistance
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant nutrient uptake and metabolism
- Keywords
- apical dominance, auxin, cytokinin biosynthesis, indole-3-acetic acid, decapitation, axillary bud outgrowth, Pisum sativum, transgenic Arabidopsis
- MeSH terms
- Cytokinins, Indoleacetic Acids, Plant Proteins, Promoter Regions, Genetic, Triiodobenzoic Acids, Arabidopsis, Gene Expression Regulation, Plant, Plant Roots, Plant Shoots, Pisum sativum, Plant Stems, Alkyl and Aryl Transferases, Plants, Genetically Modified
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