The DOI matches its Crossref record; 56 of the 56 references it cites were found in a registry or an index.
An ABRE Promoter Sequence is Involved in Osmotic Stress-Responsive Expression of the DREB2A Gene, Which Encodes a Transcription Factor Regulating Drought-Inducible Genes in Arabidopsis
Abstract
In plants, osmotic stress-responsive transcriptional regulation depends mainly on two major classes of cis-acting elements found in the promoter regions of stress-inducible genes: ABA-responsive elements (ABREs) and dehydration-responsive elements (DREs). ABRE has been shown to perceive ABA-mediated osmotic stress signals, whereas DRE is known to be involved in an ABA-independent pathway. Previously, we reported that the transcription factor DRE-BINDING PROTEIN 2A (DREB2A) regulates DRE-mediated transcription of target genes under osmotic stress conditions in Arabidopsis (Arabidopsis thaliana). However, the transcriptional regulation of DREB2A itself remains largely uncharacterized. To elucidate the transcriptional mechanism associated with the DREB2A gene under osmotic stress conditions, we generated a series of truncated and base-substituted variants of the DREB2A promoter and evaluated their transcriptional activities individually. We found that both ABRE and coupling element 3 (CE3)-like sequences located approximately -100 bp from the transcriptional initiation site are necessary for the dehydration-responsive expression of DREB2A. Coupling our transient expression analyses with yeast one-hybrid and chromatin immunoprecipitation (ChIP) assays indicated that the ABRE-BINDING PROTEIN 1 (AREB1), AREB2 and ABRE-BINDING FACTOR 3 (ABF3) bZIP transcription factors can bind to and activate the DREB2A promoter in an ABRE-dependent manner. Exogenous ABA application induced only a modest accumulation of the DREB2A transcript when compared with the osmotic stress treatment. However, the osmotic stress-induced DREB2A expression was found to be markedly impaired in several ABA-deficient and ABA-insensitive mutants. These results suggest that in addition to an ABA-independent pathway, the ABA-dependent pathway plays a positive role in the osmotic stress-responsive expression of DREB2A.
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S ALT AND D ROUGHT S TRESS S IGNAL T RANSDUCTION IN P LANTS
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Self-citationDrought and Salt Tolerance in Plants
Bartels, D., & Sunkar, R. (2005) · Critical Reviews in Plant Sciences · doi:10.1080/07352680590910410 · Cited by 2,615
ABSCISIC ACID BIOSYNTHESIS AND CATABOLISM
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Self-citationpGreen: a versatile and flexible binary Ti vector for Agrobacterium-mediated plant transformation
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Ohama, N., Sato, H., Shinozaki, K., & Yamaguchi-Shinozaki, K. (2017) · Trends in Plant Science · doi:10.1016/j.tplants.2016.08.015 · Cited by 1,234
Self-citationNakashima, K., Yamaguchi-Shinozaki, K., & Shinozaki, K. (2014) · Frontiers in Plant Science · doi:10.3389/fpls.2014.00170 · Cited by 891
Self-citationABA signaling in stress-response and seed development
Nakashima, K., & Yamaguchi-Shinozaki, K. (2013) · Plant Cell Reports · doi:10.1007/s00299-013-1418-1 · Cited by 852
Self-citationJoshi, R., Wani, S. H., Singh, B., Bohra, A., Dar, Z. A., Lone, A. A., Pareek, A., & Singla-Pareek, S. L. (2016) · Frontiers in Plant Science · doi:10.3389/fpls.2016.01029 · Cited by 849
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Metadata
- Authors
- Kim, J.-S., Mizoi, J., Yoshida, T., Fujita, Y., Nakajima, J., Ohori, T., Todaka, D., Nakashima, K., Hirayama, T., Shinozaki, K., & Yamaguchi-Shinozaki, K.
- Venue
- Plant and Cell Physiology
- Publisher
- Oxford University Press (OUP)
- Published
- 2011-10-24
- Volume
- 52
- Issue
- 12
- Pages
- 2136-2146
- ISSN
- 0032-0781 (ISSN-L)
- 1471-9053
- OpenAlex
- W2112029938
- Cited by
- 310
- References
- 56
- FWCI
- 15.62
- Type
- journal article
- Language
- en
- Open access
- closed
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- Kim, J.-S., Mizoi, J., Yoshida, T., Fujita, Y., Nakajima, J., Ohori, T., Todaka, D., Nakashima, K., Hirayama, T., Shinozaki, K., & Yamaguchi-Shinozaki, K. (2011). An ABRE Promoter Sequence is Involved in Osmotic Stress-Responsive Expression of the DREB2A Gene, Which Encodes a Transcription Factor Regulating Drought-Inducible Genes in Arabidopsis. Plant and Cell Physiology. https://doi.org/10.1093/pcp/pcr143
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