A Sodium Transporter HvHKT1;1 Confers Salt Tolerance in Barley via Regulating Tissue and Cell Ion Homeostasis
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Our previous studies showed that high salt tolerance in Tibetan wild barley accessions was associated with HvHKT1;1, a member of the high-affinity potassium transporter family. However, molecular mechanisms of HvHKT1;1 for salt tolerance and its roles in K+/Na+ homeostasis remain to be elucidated. Functional characterization of HvHKT1;1 was conducted in the present study. NaCl-induced transcripts of HvHKT1;1 were significantly higher in the roots of Tibetan wild barley XZ16 relative to other genotypes, being closely associated with its higher biomass and lower tissue Na+ content under salt stress. Heterologous expression of HvHKT1;1 in Saccharomyces cerevisiae (yeast) and Xenopus laevis oocytes showed that HvHKT1;1 had higher selectivity for Na+ over K+ and other monovalent cations. HvHKT1;1 was found to be localized at the cell plasma membrane of root stele and epidermis. Knock-down of HvHKT1;1 in barley led to higher Na+ accumulation in both roots and leaves, while overexpression of HvHKT1;1 in salt-sensitive Arabidopsis hkt1-4 and sos1-12 loss-of-function lines resulted in significantly less shoot and root Na+ accumulation. Additionally, microelectrode ion flux measurements and root elongation assay revealed that the transgenic Arabidopsis plants exhibited a remarkable capacity for regulation of Na+, K+, Ca2+ and H+ homeostasis under salt stress. These results indicate that HvHKT1;1 is critical in radial root Na+ transport, which eventually reduces shoot Na+ accumulation. Additionally, HvHKT1;1 may be indirectly involved in retention of K+ and Ca2+ in root cells, which also improves plant salt tolerance.
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Several barley genes have been exploited to address different stress conditions, including HVA1 for drought, HKT1;1 for salinity, and ARE1 for nitrogen use efficiency.
“Several barley genes have been exploited to address different stress conditions (Zhou et al., 2016;Hazzouri et al., 2018;Karunarathne et al., 2020;Mwando et al., 2020), including Hordeum vulgare aleurone 1 (HVA1) for drought, high-affinity K + transporter 1;1 (HKT1;1) for salinity, and abnormal cytokinin response 1 repressor 1 (ARE1) for nitrogen use efficiency (NUE) (Sivamani et al., 2000;Han et”
From Genetic resources and precise gene editing for targeted improvement of barley abiotic stress tolerance (2023) · cites this paper as Han et al., 2018
VIGS allows a quick study of several genes without the need for transformation.
“It allows a quick study of several genes without the need for transformation (Han et al., 2018;Feng et al., 2020b).”
From Genetic resources and precise gene editing for targeted improvement of barley abiotic stress tolerance (2023) · cites this paper as Han et al., 2018
Barley stripe mosaic virus-induced gene silencing has been conducted in barley by Han et al. (2018).
“Barley stripe mosaic virus-induced gene silencing has been conducted in barley by Han et al. (2018), and our lab has an established platform to use VIGS to back up gene selection (unpublished data).”
From Genetic resources and precise gene editing for targeted improvement of barley abiotic stress tolerance (2023) · cites this paper as Han et al. (2018)
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“…et al., 2020), including Hordeum vulgare aleurone 1 ( HVA1 ) for drought, high-affinity K + trans‐ porter 1;1 ( HKT1;1 ) for salinity, and abnormal cytoki‐ nin response 1 repressor 1 ( ARE1 ) for nitrogen use efficiency (NUE) (Sivamani et al., 2000; Han et al., 2018; Karunarathne et al., 2022).”
Self-citation
- Authors
- Han, Y., Yin, S., Huang, L., Wu, X., Zeng, J., Liu, X., Qiu, L., Munns, R., Chen, Z.-H., & Zhang, G.
- Affiliations
- Zhejiang University (CN)
- Western Sydney University (AU)
- The University of Western Australia (AU)
- Corresponding author
- Zhang, Guoping
- ORCID
- Venue
- Plant and Cell Physiology
- Publisher
- Oxford University Press (OUP)
- Published
- 2018-06-15
- Volume
- 59
- Issue
- 10
- Pages
- 1976-1989
- ISSN
- 0032-0781 (ISSN-L)
- 1471-9053
- OpenAlex
- W2808856902
- Cited by
- 85
- References
- 54
- Citations by year
- 2026: 5 · 2025: 9 · 2024: 12 · 2023: 13 · 2022: 9 · 2021: 12 · 2020: 17 · 2019: 7 · 2018: 1
- FWCI
- 8.17
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model (vor)
- Locations
- Plant and Cell Physiology (publishedVersion)
- PubMed (publishedVersion)
- Murdoch University Research Portal (submittedVersion)
- UWA Profiles and Research Repository (UWA) (submittedVersion, other-oa)
- Funders
- National Natural Science Foundation of China (31501296, 31571578, 31620103912)
- China Agriculture Research System (CARS-05)
- Australian Research Council
- Discovery Early Career Researcher (DE1401011143)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Stress Responses and Tolerance
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant nutrient uptake and metabolism
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Ion Transport and Channel Regulation
- Keywords
- salt tolerance, barley, sodium transport, Na+/K+ homeostasis, high-affinity potassium transporter, Tibetan wild barley, NaCl stress, ion homeostasis, ion flux, heterologous expression, plasma membrane localization, root stele
- MeSH terms
- Animals, Hordeum, Plant Proteins, Saccharomyces cerevisiae, Sodium, Xenopus laevis, Arabidopsis, Gene Expression Regulation, Plant, Plants, Genetically Modified, Salt Tolerance
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