Changes in plasma free fatty acid concentrations in rheumatoid arthritis patients during fasting and their effects upon T-lymphocyte proliferation
Abstract
OBJECTIVE: To measure whether changes in the concentrations of circulating free fatty acids (FFAs) after a 7 day fast in rheumatoid arthritis (RA) patients would inhibit in vitro T-lymphocyte proliferation. METHODS: The concentration and composition of plasma FFAs were measured in nine RA patients at the conclusion of a 7 day fast. A FFA mixture was made up based on these findings (20% linoleic, 43% oleic, 10% stearic, 27% palmitic acid). Mitogen-induced lymphocyte proliferative responses were measured after co-culture of peripheral blood mononuclear cells (PBMC) from healthy individuals in the presence of increasing concentrations of this FFA mixture (from 0 to 2000 microM) and in the presence of FFA mixtures where the relative proportions of fatty acids varied. RESULTS: Both the concentration of the FFA mixture and the ratio between the unsaturated and saturated fatty acids significantly influenced in vitro lymphocyte proliferation (P<0.0001). Unexpectedly, the highest concentrations of the FFA mixture increased lymphocyte proliferation. At equimolar concentrations (600 microM), manipulating the amounts of oleic and linoleic fatty acids relative to stearic and palmitic fatty acids had a potent inhibitory effect upon lymphocyte proliferation. CONCLUSION: Fasting-associated increases in total plasma FFA concentrations do not inhibit, but rather enhance, in vitro lymphocyte proliferation. An inhibitory effect could only be achieved by manipulating the balance between the unsaturated and saturated fatty acids.
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In humans, average concentration of FFAs in postabsorptive state is 500 to 1,000 μM in plasma, and that of PA can reach 200 μM.
“In humans, the average concentration of FFAs in the postabsorptive state is 500 to 1,000 μM in the plasma, and that of PA can reach 200 μM (17).”
From Mitochondrial Fission Contributes to Mitochondrial Dysfunction and Insulin Resistance in Skeletal Muscle (2012) · cites this paper as [17]
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- Authors
- Fraser, D. A., Thoen, J., Rustan, A. C., Førre, Ø., & Kjeldsen-Kragh, J.
- Affiliations
- Oslo University Hospital (NO)
- Corresponding author
- Fraser, D. A.
- Venue
- Rheumatology
- Publisher
- Oxford University Press (OUP)
- Published
- 1999-10
- Volume
- 38
- Issue
- 10
- Pages
- 948-952
- ISSN
- 1462-0324 (ISSN-L)
- 1462-0332
- OpenAlex
- W2165441472
- Cited by
- 50
- References
- 19
- Citations by year
- 2025: 1 · 2024: 3 · 2023: 1 · 2022: 2 · 2021: 3 · 2020: 3 · 2019: 3 · 2018: 2 · 2017: 2 · 2016: 3 · 2015: 6 · 2014: 3 · 2013: 3 · 2012: 2
- FWCI
- 0.62
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Lara D. Veeken (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Nursing › Nutrition and Dietetics › Fatty Acid Research and Health
- Health Sciences › Medicine › Rheumatology › Rheumatoid Arthritis Research and Therapies
- Health Sciences › Medicine › Physiology › Dietary Effects on Health
- Keywords
- rheumatoid arthritis, plasma free fatty acids, fasting, T cell proliferation, unsaturated fatty acids, saturated fatty acids, linoleic acid, oleic acid, stearic acid, palmitic acid, fatty acid composition, lymphocyte proliferation inhibition
- MeSH terms
- Adult, Aged, Arthritis, Rheumatoid, Cell Division, Cell Survival, Dose-Response Relationship, Drug, Fasting, Fatty Acids, Fatty Acids, Nonesterified, Fatty Acids, Unsaturated, Female, Humans, Male, Middle Aged, T-Lymphocytes, In Vitro Techniques
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