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Cited by 43Plant Pathogens and Fungal DiseasesFungal and yeast genetics researchPlant pathogens and resistance mechanisms

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Role of Nitrogen-Metabolism Genes Expressed During Pathogenicity of the Alkalinizing Colletotrichum gloeosporioides and Their Differential Expression in Acidifying Pathogens

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Miyara, I., Shnaiderman, C., Meng, X., Vargas, W. A., Diaz-Minguez, J. M., Sherman, A., Thon, M., Prusky, D.·2012·Molecular Plant-Microbe Interactions®
DOI 10.1094/mpmi-01-12-0017-r
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Abstract

Pathogens can actively alter fruit pH around the infection site, signaling modulation of pathogenicity-factor expression, as found for alkalinizing (Colletotrichum and Alternaria spp.) and acidifying (Penicillium, Botrytis, and Sclerotinia spp.) fungi. The nitrogen-metabolism genes GDH2, GS1, GLT, and MEP genes are differentially expressed during colonization by Colletotrichum gloeosporioides, and a Δgdh2 strain reduces ammonia accumulation and pathogenicity. We analyzed the contribution of transporters GLT and MEPB to C. gloeosporiodes pathogenicity. Germinating spores of Δglt strains showed reduced appressorium formation; those of ΔmepB mutants showed rapid ammonia uptake and accumulation inside the hyphae, indicating deregulated uptake. Both mutants reduced pathogenicity, indicating that these transporters function during alkalinizing species pathogenicity. We compared the expressions of these genes in C. gloeosporioides and Sclerotinia sclerotiorum, and found five to 10-fold higher expression at the transcript level in the former. Interestingly, GLT and MEPB in the alkalinizing species showed no and very low sequence identity, respectively, with their counterparts in the acidifying species. Knockout analysis of GLT and MEPB and their differential transcript regulation in the alkalinizing and acidifying species suggest that the ammonia accumulation contributing to pathogenicity in the former is modulated by factors at the gene-regulation levels that are lacking in the acidifying species.

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Metadata

Authors
Miyara, I., Shnaiderman, C., Meng, X., Vargas, W. A., Diaz-Minguez, J. M., Sherman, A., Thon, M., & Prusky, D.
Affiliations
  • Agricultural Research Organization (IL)
  • Hebrew University of Jerusalem (IL)
  • Guangdong Academy of Agricultural Sciences (CN)
  • Universidad de Salamanca (ES)
Corresponding author
Prusky, D.
ORCID
Venue
Molecular Plant-Microbe Interactions®
Publisher
Scientific Societies
Published
2012-09
Volume
25
Issue
9
Pages
1251-1263
ISSN
  • 0894-0282 (ISSN-L)
  • 1943-7706
DOI
10.1094/mpmi-01-12-0017-r
OpenAlex
W2030198301
Cited by
43
References
0
Citations by year
2025: 3 · 2024: 2 · 2023: 5 · 2022: 3 · 2021: 1 · 2020: 1 · 2019: 1 · 2018: 2 · 2017: 4 · 2016: 5 · 2015: 1 · 2014: 5 · 2013: 8 · 2012: 2
FWCI
2.42
Type
journal article
Language
en
Open access
Locations
  • Molecular Plant-Microbe Interactions (publishedVersion)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Funders
United States - Israel Binational Agricultural Research and Development Fund (IS-3945-06)
Topics
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Pathogens and Fungal Diseases
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant pathogens and resistance mechanisms
Keywords
Colletotrichum gloeosporioides, Sclerotinia sclerotiorum, nitrogen metabolism, ammonia accumulation, GDH2, glutamine synthetase, gene expression, pathogenicity, appressorium formation, fruit pH, gene expression regulation, plant pathogenic fungi
MeSH terms
Amino Acid Sequence, Ammonia, Ascomycota, Carrier Proteins, Fungal Proteins, Hydrogen-Ion Concentration, Molecular Sequence Data, Nitrogen, Phylogeny, Gene Expression Regulation, Fungal

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