The DOI matches its Crossref record; 51 of the 51 references it cites were found in a registry or an index.
A Draft Genome Sequence of Nicotiana benthamiana to Enhance Molecular Plant-Microbe Biology Research
Open accessAbstract
Nicotiana benthamiana is a widely used model plant species for the study of fundamental questions in molecular plant-microbe interactions and other areas of plant biology. This popularity derives from its well-characterized susceptibility to diverse pathogens and, especially, its amenability to virus-induced gene silencing and transient protein expression methods. Here, we report the generation of a 63-fold coverage draft genome sequence of N. benthamiana and its availability on the Sol Genomics Network for both BLAST searches and for downloading to local servers. The estimated genome size of N. benthamiana is 3 Gb (gigabases). The current assembly consists of approximately 141,000 scaffolds, spanning 2.6 Gb with 50% of the genome sequence contained within scaffolds >89 kilobases. Of the approximately 16,000 N. benthamiana unigenes available in GenBank, >90% are represented in the assembly. The usefulness of the sequence was demonstrated by the retrieval of N. benthamiana orthologs for 24 immunity-associated genes from other species including Ago2, Ago7, Bak1, Bik1, Crt1, Fls2, Pto, Prf, Rar1, and mitogen-activated protein kinases. The sequence will also be useful for comparative genomics in the Solanaceae family as shown here by the discovery of microsynteny between N. benthamiana and tomato in the region encompassing the Pto and Prf genes.
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The Sol Genomics Network (SGN)—from genotype to phenotype to breeding
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Self-citationPlant gene editing through de novo induction of meristems
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Self-citationMicroRNAs from the parasitic plant Cuscuta campestris target host messenger RNAs
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Senthil-Kumar, M., & Mysore, K. S. (2014) · Nature Protocols · doi:10.1038/nprot.2014.092 · Cited by 381
Metadata
- Authors
- Bombarely, A., Rosli, H. G., Vrebalov, J., Moffett, P., Mueller, L. A., & Martin, G. B.
- Venue
- Molecular Plant-Microbe Interactions®
- Publisher
- Scientific Societies
- Published
- 2012-12
- Volume
- 25
- Issue
- 12
- Pages
- 1523-1530
- ISSN
- 0894-0282 (ISSN-L)
- 1943-7706
- OpenAlex
- W2092053018
- Cited by
- 472
- References
- 51
- FWCI
- 12.31
- Type
- journal article
- Language
- en
- Open access
- green
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- Bombarely, A., Rosli, H. G., Vrebalov, J., Moffett, P., Mueller, L. A., & Martin, G. B. (2012). A Draft Genome Sequence of Nicotiana benthamiana to Enhance Molecular Plant-Microbe Biology Research. Molecular Plant-Microbe Interactions®. https://doi.org/10.1094/mpmi-06-12-0148-ta
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