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Quality control and preprocessing of metagenomic datasets

Open access
Schmieder, Robert, Edwards, Robert·2011·Bioinformatics

Abstract

Summary: Here, we present PRINSEQ for easy and rapid quality control and data preprocessing of genomic and metagenomic datasets. Summary statistics of FASTA (and QUAL) or FASTQ files are generated in tabular and graphical form and sequences can be filtered, reformatted and trimmed by a variety of options to improve downstream analysis. Availability and Implementation: This open-source application was implemented in Perl and can be used as a stand alone version or accessed online through a user-friendly web interface. The source code, user help and additional information are available at http://prinseq.sourceforge.net/. Contact: rschmied@sciences.sdsu.edu; redwards@cs.sdsu.edu

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Research works printed but not linked: 6

  1. Blankenberg, 2010, Manipulation of FASTQ data with Galaxy, Bioinformatics, 26, 1783, 10.1093/bioinformatics/btq281 · DOI · Paper page
  2. Burge, 1992, Over- and under-representation of short oligonucleotides in DNA sequences, Proc. Natl Acad. Sci. USA, 89, 1358, 10.1073/pnas.89.4.1358 · DOI · Paper page
  3. Gomez-Alvarez, 2009, Systematic artifacts in metagenomes from complex microbial communities, ISME J., 3, 1314, 10.1038/ismej.2009.72 · DOI · Paper page
  4. Morgulis, 2006, A fast and symmetric DUST implementation to mask low-complexity DNA sequences, J. Comput. Biol., 13, 1028, 10.1089/cmb.2006.13.1028 · DOI · Paper page
  5. Schmieder, 2010, TagCleaner: identification and removal of tag sequences from genomic and metagenomic datasets, BMC Bioinformatics, 11, 341, 10.1186/1471-2105-11-341 · DOI · Paper page
  6. Willner, 2009, Metagenomic signatures of 86 microbial and viral metagenomes, Envir. Microbiol., 11, 1752, 10.1111/j.1462-2920.2009.01901.x · DOI

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Metadata

Authors
Schmieder, R., & Edwards, R.
Venue
Bioinformatics
Published
2011-01-28
Volume
27
Issue
6
Pages
863-864
ISSN
  • 1367-4803 (ISSN-L)
  • 1367-4811
OpenAlex
W2121180999
Cited by
5,072
References
1
FWCI
32.45
Type
journal article
Language
en
Open access
hybrid

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Schmieder, R., & Edwards, R. (2011). Quality control and preprocessing of metagenomic datasets. Bioinformatics. https://doi.org/10.1093/bioinformatics/btr026

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