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Small, Stable Shuttle Vectors Based on the Minimal pVS1 Replicon for Use in Gram-Negative, Plant-Associated Bacteria

Open access
Heeb, Stephan, Itoh, Yoshifumi, Nishijyo, Takayuki, Schnider, Ursula, Keel, Christoph, Wade, Julie, Walsh, Ultan, O'Gara, Fergal, Haas, Dieter·2000·Molecular Plant-Microbe Interactions®

Abstract

The minimal replicon of the Pseudomonas plasmid pVS1 was genetically defined and combined with the Escherichia coli p15A replicon, to provide a series of new, oligocopy cloning vectors (5.3 to 8.3 kb). Recombinant plasmids derived from these vectors were stable in growing and nongrowing cells of root-colonizing P. fluorescens strains incubated under different environmental conditions for more than 1 month.

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Crossref · 10.1094/mpmi.2000.13.2.232

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Metadata

Authors
Heeb, S., Itoh, Y., Nishijyo, T., Schnider, U., Keel, C., Wade, J., Walsh, U., O'Gara, F., & Haas, D.
Venue
Molecular Plant-Microbe Interactions®
Published
2000-02
Volume
13
Issue
2
Pages
232-237
ISSN
  • 0894-0282 (ISSN-L)
  • 1943-7706
OpenAlex
W2084879740
Cited by
367
References
0
FWCI
8.35
Type
journal article
Language
en
Open access
green

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CoChat. (2026). Citation check: Small, Stable Shuttle Vectors Based on the Minimal pVS1 Replicon for Use in Gram-Negative, Plant-Associated Bacteria. https://app.cochat.ai/papers/10.1094/mpmi.2000.13.2.232
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Heeb, S., Itoh, Y., Nishijyo, T., Schnider, U., Keel, C., Wade, J., Walsh, U., O'Gara, F., & Haas, D. (2000). Small, Stable Shuttle Vectors Based on the Minimal pVS1 Replicon for Use in Gram-Negative, Plant-Associated Bacteria. Molecular Plant-Microbe Interactions®. https://doi.org/10.1094/mpmi.2000.13.2.232

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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).

Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.