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Cited by 308

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Chitinolytic Enzymes of Trichoderma harzianum: Purification of Chitobiosidase and Endochitinase

Harman, G. E.·1993·Phytopathology

Abstract

Trichoderma harzianum strain P1 produces a variety of chitinolytic enzymes including N-acetyl-β-D-glucosaminidases, chitin 1,4-β-chitobiosidases, and an endochitinase. Chitabiosidases and an endochitinase were purified from dialyzed, concentrated culture filtrates using gel filtration, chromatofocusing, and isoelectric focusing. Three protein bands were evident in the purified chitobiosidase preparation, representing different levels of N-glycosylation of the same protein. The pI of all purified proteins was ∼3.9 []

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Authors
Harman, G. E.
Venue
Phytopathology
Published
1993
Volume
83
Issue
3
Pages
313
ISSN
0031-949X (ISSN-L)
OpenAlex
W2001603167
Cited by
308
References
0
FWCI
3.12
Type
journal article
Language
en
Open access
closed

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