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Long-Term Proliferation in Culture and Germline Transmission of Mouse Male Germline Stem Cells1
Abstract
Spermatogenesis is a complex process that originates in a small population of spermatogonial stem cells. Here we report the in vitro culture of spermatogonial stem cells that proliferate for long periods of time. In the presence of glial cell line-derived neurotrophic factor, epidermal growth factor, basic fibroblast growth factor, and leukemia inhibitory factor, gonocytes isolated from neonatal mouse testis proliferated over a 5-month period (>10(14)-fold) and restored fertility to congenitally infertile recipient mice following transplantation into seminiferous tubules. Long-term spermatogonial stem cell culture will be useful for studying spermatogenesis mechanism and has important implications for developing new technology in transgenesis or medicine.
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Cited by
Generation of Pluripotent Stem Cells from Neonatal Mouse Testis
Kanatsu-Shinohara, M., Inoue, K., Lee, J., Yoshimoto, M., Ogonuki, N., Miki, H., Baba, S., Kato, T., Kazuki, Y., Toyokuni, S., et al. (2004) · Cell · doi:10.1016/j.cell.2004.11.011 · Cited by 814
Self-citationEffects of Aging and Niche Microenvironment on Spermatogonial Stem Cell Self-Renewal
Ryu, B.-Y., Orwig, K. E., Oatley, J. M., Avarbock, M. R., & Brinster, R. L. (2006) · Stem Cells · doi:10.1634/stemcells.2005-0580 · Cited by 265
Wu, Y., Zhou, H., Fan, X., Zhang, Y., Zhang, M., Wang, Y., Xie, Z., Bai, M., Yin, Q., Liang, D., et al. (2015) · Cell Research · doi:10.1038/cr.2014.160 · Cited by 247
In vitro production of fertile sperm from murine spermatogonial stem cell lines
Sato, T., Katagiri, K., Yokonishi, T., Kubota, Y., Inoue, K., Ogonuki, N., Matoba, S., Ogura, A., & Ogawa, T. (2011) · Nature Communications · doi:10.1038/ncomms1478 · Cited by 228
Self-citationEffects of Growth Factors on In Vitro Culture of Neonatal Piglet Testicular Tissue Fragments
Ibtisham, F., Cham, T.-C., Fayaz, M. A., & Honaramooz, A. (2023) · Cells · doi:10.3390/cells12182234 · Cited by 12
Metadata
- Authors
- Kanatsu-Shinohara, M., Ogonuki, N., Inoue, K., Miki, H., Ogura, A., Toyokuni, S., & Shinohara, T.
- Venue
- Biology of Reproduction
- Publisher
- Oxford University Press (OUP)
- Published
- 2003-08-01
- Volume
- 69
- Issue
- 2
- Pages
- 612-616
- ISSN
- 0006-3363 (ISSN-L)
- 1529-7268
- OpenAlex
- W2131238404
- Cited by
- 1,048
- References
- 0
- FWCI
- 21.41
- Type
- journal article
- Language
- en
- Open access
- closed
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- Kanatsu-Shinohara, M., Ogonuki, N., Inoue, K., Miki, H., Ogura, A., Toyokuni, S., & Shinohara, T. (2003). Long-Term Proliferation in Culture and Germline Transmission of Mouse Male Germline Stem Cells1. Biology of Reproduction. https://doi.org/10.1095/biolreprod.103.017012
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