The DOI matches its Crossref record; 15 of the 15 references it cites were found in a registry or an index.
Feeder Layer- and Serum-Free Culture of Human Embryonic Stem Cells1
Abstract
In addition to their contribution to the research on early human development, human embryonic stem (hES) cells may also be used for cell-based therapies. Traditionally, these cells have been cultured on mouse embryonic fibroblast feeder layers, which allow their continuous growth in an undifferentiated state. However, the use of hES cells in human therapy requires an animal-free culture system, in which exposure to mouse retroviruses is avoided. In this study we present a novel feeder layer-free culture system for hES cells, based on medium supplemented with 15% serum replacement, a combination of growth factors including transforming growth factor beta1 (TGFbeta1), leukemia inhibitory factor, basic fibroblast growth factor, and fibronectin matrix. Human ES cells grown in these conditions maintain all ES cell features after prolonged culture, including the developmental potential to differentiate into representative tissues of the three embryonic germ layers, unlimited and undifferentiated proliferative ability, and maintenance of normal karyotype. The culture system presented here has two major advantages: 1) application of a well-defined culture system for hES cells and 2) reduced exposure of hES cells to animal pathogens. The feeder layer-free culture system reported here aims at facilitating research practices and providing a safer alternative for future clinical applications of hES cells.
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Citations
Cites
Embryonic Stem Cell Lines Derived from Human Blastocysts
Thomson, J. A., Itskovitz-Eldor, J., Shapiro, S. S., Waknitz, M. A., Swiergiel, J. J., Marshall, V. S., & Jones, J. M. (1998) · Science · doi:10.1126/science.282.5391.1145 · Cited by 15,926
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Embryonic stem cell lines from human blastocysts: somatic differentiation in vitro
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Myeloid leukaemia inhibitory factor maintains the developmental potential of embryonic stem cells
Williams, R. L., Hilton, D. J., Pease, S., Willson, T. A., Stewart, C. L., Gearing, D. P., Wagner, E. F., Metcalf, D., Nicola, N. A., & Gough, N. M. (1988) · Nature · doi:10.1038/336684a0 · Cited by 1,997
Feeder-free growth of undifferentiated human embryonic stem cells
Xu, C., Inokuma, M. S., Denham, J., Golds, K., Kundu, P., Gold, J. D., & Carpenter, M. K. (2001) · Nature Biotechnology · doi:10.1038/nbt1001-971 · Cited by 1,870
"Stemness": Transcriptional Profiling of Embryonic and Adult Stem Cells
Ramalho-Santos, M., Yoon, S., Matsuzaki, Y., Mulligan, R. C., & Melton, D. A. (2002) · Science · doi:10.1126/science.1072530 · Cited by 1,709
Itskovitz-Eldor, J., Schuldiner, M., Karsenti, D., Eden, A., Yanuka, O., Amit, M., Soreq, H., & Benvenisty, N. (2000) · Molecular Medicine · doi:10.1007/bf03401776 · Cited by 1,500
Self-citationAmit, M., Carpenter, M. K., Inokuma, M. S., Chiu, C.-P., Harris, C. P., Waknitz, M. A., Itskovitz-Eldor, J., & Thomson, J. A. (2000) · Developmental Biology · doi:10.1006/dbio.2000.9912 · Cited by 1,472
Self-citationSchuldiner, M., Yanuka, O., Itskovitz-Eldor, J., Melton, D. A., & Benvenisty, N. (2000) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.97.21.11307 · Cited by 1,134
Self-citationIsolation of a primate embryonic stem cell line.
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Cited by
Efficient differentiation of human embryonic stem cells to definitive endoderm
D'Amour, K. A., Agulnick, A. D., Eliazer, S., Kelly, O. G., Kroon, E., & Baetge, E. E. (2005) · Nature Biotechnology · doi:10.1038/nbt1163 · Cited by 1,730
Derivation of human embryonic stem cells in defined conditions
Ludwig, T. E., Levenstein, M. E., Jones, J. M., Berggren, W. T., Mitchen, E. R., Frane, J. L., Crandall, L. J., Daigh, C. A., Conard, K. R., Piekarczyk, M. S., et al. (2006) · Nature Biotechnology · doi:10.1038/nbt1177 · Cited by 1,114
Human embryonic stem cells express an immunogenic nonhuman sialic acid
Martin, M. J., Muotri, A., Gage, F., & Varki, A. (2005) · Nature Medicine · doi:10.1038/nm1181 · Cited by 932
Feeder-independent culture of human embryonic stem cells
Ludwig, T. E., Bergendahl, V., Levenstein, M. E., Yu, J., Probasco, M. D., & Thomson, J. A. (2006) · Nature Methods · doi:10.1038/nmeth902 · Cited by 681
NANOG Is a Direct Target of TGFβ/Activin-Mediated SMAD Signaling in Human ESCs
Xu, R.-H., Sampsell-Barron, T. L., Gu, F., Root, S., Peck, R. M., Pan, G., Yu, J., Antosiewicz-Bourget, J., Tian, S., Stewart, R., et al. (2008) · Cell Stem Cell · doi:10.1016/j.stem.2008.07.001 · Cited by 511
Metadata
- Authors
- Amit, M., Shariki, C., Margulets, V., & Itskovitz-Eldor, J.
- Venue
- Biology of Reproduction
- Publisher
- Oxford University Press (OUP)
- Published
- 2004-03-01
- Volume
- 70
- Issue
- 3
- Pages
- 837-845
- ISSN
- 0006-3363 (ISSN-L)
- 1529-7268
- OpenAlex
- W2151487778
- Cited by
- 664
- References
- 15
- FWCI
- 25.37
- Type
- journal article
- Language
- en
- Open access
- closed
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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