CoChat
Sign Up
Cited by 189Reproductive Biology and FertilityCancer, Hypoxia, and MetabolismReproductive Physiology in Livestock

The DOI matches its Crossref record; 66 of the 66 references it cites were found in a registry or an index.

Oxygen-Regulated Gene Expression in Bovine Blastocysts1

Harvey, A.J., Kind, K.L., Pantaleon, M., Armstrong, D.T., Thompson, J.G.·2004·Biology of Reproduction
DOI 10.1095/biolreprod.104.028639
Open this paper in CoChat — free account

Abstract

Oxygen concentrations used during in vitro embryo culture can influence embryo development, cell numbers, and gene expression. Here we propose that the preimplantation bovine embryo possesses a molecular mechanism for the detection of, and response to, oxygen, mediated by a family of basic helix-loop-helix transcription factors, the hypoxia-inducible factors (HIFs). Day 5 compacting bovine embryos were cultured under different oxygen tensions (2%, 7%, 20%) and the effect on the expression of oxygen-regulated genes, development, and cell number allocation and HIFalpha protein localization were examined. Bovine in vitro-produced embryos responded to variations in oxygen concentration by altering gene expression. GLUT1 expression was higher following 2% oxygen culture compared with 7% and 20% cultured blastocysts. HIF mRNA expression (HIF1alpha, HIF2alpha) was unaltered by oxygen concentration. HIF2alpha protein was predominantly localized to the nucleus of blastocysts. In contrast, HIF1alpha protein was undetectable at any oxygen concentration or in the presence of the HIF protein stabilizer desferrioxamine (DFO), despite being detectable in cumulus cells following normal maturation conditions, acute anoxic culture, or in the presence of DFO. Oxygen concentration also significantly altered inner cell mass cell proportions at the blastocyst stage. These results suggest that oxygen can influence gene expression in the bovine embryo during postcompaction development and that these effects may be mediated by HIF2alpha.

Overview

Integrity signals

Registry

Crossref · 10.1095/biolreprod.104.028639

Cites

66 of 66 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Verification

Claims the paper makes

This paper’s full text isn’t openly available, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Citations

Cites

Show all 66 references

Research works printed but not linked: 2

  1. 2003, Proceedings of the 34th annual conference of the Society for Reproductive Biology
  2. 2003, Proceedings of the 2nd world congress on fetal origins of adult disease

Cited by

Sorted by citations

See all 189

Related

Metadata

Authors
Harvey, A. J., Kind, K. L., Pantaleon, M., Armstrong, D. T., & Thompson, J. G.
Affiliations
  • Queen Elizabeth Hospital (AU)
  • The University of Queensland (AU)
Corresponding author
Harvey, A.J.
ORCID
Venue
Biology of Reproduction
Publisher
Oxford University Press (OUP)
Published
2004-10-01
Volume
71
Issue
4
Pages
1108-1119
ISSN
  • 0006-3363 (ISSN-L)
  • 1529-7268
DOI
10.1095/biolreprod.104.028639
OpenAlex
W2154875545
Cited by
189
References
66
Citations by year
2026: 1 · 2025: 4 · 2024: 4 · 2023: 2 · 2022: 14 · 2021: 6 · 2020: 15 · 2019: 4 · 2018: 4 · 2017: 10 · 2016: 6 · 2015: 13 · 2014: 4 · 2013: 13 · 2012: 9
FWCI
12.56
Type
journal article
Language
en
Open access
closed
Locations
  • Biology of Reproduction (publishedVersion)
  • PubMed (publishedVersion)
  • BioOne Complete (BioOne) (submittedVersion)
  • Adelaide Research & Scholarship (AR&S) (University of Adelaide) (submittedVersion)
Funders
  • Medical Research Council
  • National Health and Medical Research Council (157941)
Topics
  • Health Sciences › Medicine › Reproductive Medicine › Reproductive Biology and Fertility
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › Cancer, Hypoxia, and Metabolism
  • Life Sciences › Agricultural and Biological Sciences › Animal Science and Zoology › Reproductive Physiology in Livestock
Keywords
oxygen regulation, bovine blastocyst, hypoxia-inducible factor, HIF-1α, HIF-2α, GLUT1, embryo culture, oxygen partial pressure, deferoxamine, inner cell mass, bHLH transcription factors, embryonic development, cumulus cells
MeSH terms
Endothelial PAS Domain-Containing Protein 1, Animals, Blastocyst, Cattle, Monosaccharide Transport Proteins, Oxygen, RNA, Messenger, Tissue Distribution, Transcription Factors, Gene Expression Regulation, Developmental, Vascular Endothelial Growth Factor A, Embryo Culture Techniques, Embryonic Development, Glucose Transporter Type 1, Basic Helix-Loop-Helix Proteins, Hypoxia-Inducible Factor 1, alpha Subunit

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review