Cited by 189Reproductive Biology and FertilityCancer, Hypoxia, and MetabolismReproductive Physiology in Livestock

The DOI matches its Crossref record; 66 of the 66 references it cites were found in a registry or an index.

Oxygen-Regulated Gene Expression in Bovine Blastocysts1

Harvey, A.J., Kind, K.L., Pantaleon, M., Armstrong, D.T., Thompson, J.G.·2004·Biology of Reproduction

Open this paper in CoChat — free account

Abstract

Oxygen concentrations used during in vitro embryo culture can influence embryo development, cell numbers, and gene expression. Here we propose that the preimplantation bovine embryo possesses a molecular mechanism for the detection of, and response to, oxygen, mediated by a family of basic helix-loop-helix transcription factors, the hypoxia-inducible factors (HIFs). Day 5 compacting bovine embryos were cultured under different oxygen tensions (2%, 7%, 20%) and the effect on the expression of oxygen-regulated genes, development, and cell number allocation and HIFalpha protein localization were examined. Bovine in vitro-produced embryos responded to variations in oxygen concentration by altering gene expression. GLUT1 expression was higher following 2% oxygen culture compared with 7% and 20% cultured blastocysts. HIF mRNA expression (HIF1alpha, HIF2alpha) was unaltered by oxygen concentration. HIF2alpha protein was predominantly localized to the nucleus of blastocysts. In contrast, HIF1alpha protein was undetectable at any oxygen concentration or in the presence of the HIF protein stabilizer desferrioxamine (DFO), despite being detectable in cumulus cells following normal maturation conditions, acute anoxic culture, or in the presence of DFO. Oxygen concentration also significantly altered inner cell mass cell proportions at the blastocyst stage. These results suggest that oxygen can influence gene expression in the bovine embryo during postcompaction development and that these effects may be mediated by HIF2alpha.

Integrity signals

Registry

Crossref · 10.1095/biolreprod.104.028639

Cites

66 of 66 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Claims the paper makes

This paper’s full text isn’t openly available, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Hypoxia-inducible factor 1 is a basic-helix-loop-helix-PAS heterodimer regulated by cellular O2 tension.
Wang, G. L., Jiang, B. H., Rue, E. A., & Semenza, G. L. (1995) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.92.12.5510 · Cited by 6,212
Heterozygous embryonic lethality induced by targeted inactivation of the VEGF gene
Ferrara, N., Carver-Moore, K., Chen, H., Dowd, M., Lu, L., O’Shea, K. S., Powell-Braxton, L., Hillan, K. J., & Moore, M. W. (1996) · Nature · doi:10.1038/380439a0 · Cited by 3,661
A conserved family of prolyl-4-hydroxylases that modify HIF
Bruick, R. K., & McKnight, S. L. (2001) · Science · doi:10.1126/science.1066373 · Cited by 2,549
Cellular and developmental control of O 2 homeostasis by hypoxia-inducible factor 1α
Iyer, N. V., Kotch, L. E., Agani, F., Leung, S. W., Laughner, E., Wenger, R. H., Gassmann, M., Gearhart, J. D., Lawler, A. M., Yu, A. Y., et al. (1998) · Genes & Development · doi:10.1101/gad.12.2.149 · Cited by 2,476
Transcriptional regulation of genes encoding glycolytic enzymes by hypoxia-inducible factor 1.
Semenza, G., Roth, P. H., Fang, H. M., & Wang, G. L. (1994) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(17)31580-6 · Cited by 1,892
Molecular and Biological Properties of the Vascular Endothelial Growth Factor Family of Proteins
Ferrara, N., Houck, K. A., Jakeman, L. B., & LEUNG, D. W. (1992) · Endocrine Reviews · doi:10.1210/edrv-13-1-18 · Cited by 1,655
HIF‐1α is required for solid tumor formation and embryonic vascularization
Ryan, H. E., Lo, J., & Johnson, R. S. (1998) · The EMBO Journal · doi:10.1093/emboj/17.11.3005 · Cited by 1,579
Asparagine Hydroxylation of the HIF Transactivation Domain: A Hypoxic Switch
Lando, D., Peet, D. J., Whelan, D. A., Gorman, J. J., & Whitelaw, M. L. (2002) · Science · doi:10.1126/science.1068592 · Cited by 1,558
Oxidative stress and protection against reactive oxygen species in the pre-implantation embryo and its surroundings
Guerin, P. J. (2001) · Human Reproduction Update · doi:10.1093/humupd/7.2.175 · Cited by 1,419
HIF-1 and mechanisms of hypoxia sensing
Semenza, G. L. (2001) · Current Opinion in Cell Biology · doi:10.1016/s0955-0674(00)00194-0 · Cited by 1,301
Hypoxia-inducible factor 1 levels vary exponentially over a physiologically relevant range of O2 tension
Jiang, B. H., Semenza, G. L., Bauer, C., & Marti, H. H. (1996) · American Journal of Physiology-Cell Physiology · doi:10.1152/ajpcell.1996.271.4.c1172 · Cited by 1,213
Hypoxia-inducible factor 1: master regulator of O2 homeostasis
Semenza, G. L. (1998) · Current Opinion in Genetics & Development · doi:10.1016/s0959-437x(98)80016-6 · Cited by 1,092
SUCCESSFUL CULTURE IN VITRO OF SHEEP AND CATTLE OVA
Tervit, H. R., Whittingham, D. G., & ROWSON, L. E. A. (1972) · Reproduction · doi:10.1530/jrf.0.0300493 · Cited by 1,008
Signal transduction to hypoxia-inducible factor 1
Semenza, G. L. (2002) · Biochemical Pharmacology · doi:10.1016/s0006-2952(02)01168-1 · Cited by 860
Differential Transcriptional Regulation of the Two Vascular Endothelial Growth Factor Receptor Genes
Gerber, H.-P., Condorelli, F., Park, J., & Ferrara, N. (1997) · Journal of Biological Chemistry · doi:10.1074/jbc.272.38.23659 · Cited by 794
Oxygen tension in the oviduct and uterus of rhesus monkeys, hamsters and rabbits
Fischer, B., & Bavister, B. D. (1993) · Reproduction · doi:10.1530/jrf.0.0990673 · Cited by 771
Widespread, hypoxia‐inducible expression of HIF‐2α in distinct cell populations of different organs
Wiesener, M. S., Jürgensen, J. S., Rosenberger, C., Scholze, C. K., Hörstrup, J. H., Warnecke, C., Mandriota, S. J., Bechmann, I., Frei, U. A., Pugh, C. W., et al. (2002) · The FASEB Journal · doi:10.1096/fj.02-0445fje · Cited by 770
Desferrioxamine induces erythropoietin gene expression and hypoxia- inducible factor 1 DNA-binding activity: implications for models of hypoxia signal transduction
Wang, G., & Semenza, G. (1993) · Blood · doi:10.1182/blood.v82.12.3610.3610 · Cited by 766
HIF‐1 is expressed in normoxic tissue and displays an organ‐specific regulation under systemic hypoxia
Stroka, D., Burkhardt, T., Desbaillets, I., Wenger, R. H., Neil, D., Bauer, C., GASSMANN, M., & Candinas, D. (2001) · The FASEB Journal · doi:10.1096/fj.01-0125com · Cited by 727
Hypoxia-inducible factor-1 mediates the biological effects of oxygen on human trophoblast differentiation through TGFβ3
Caniggia, I., Mostachfi, H., Winter, J., Gassmann, M., Lye, S. J., Kuliszewski, M., & Post, M. (2000) · Journal of Clinical Investigation · doi:10.1172/jci8316 · Cited by 644
Induction of Endothelial PAS Domain Protein-1 by Hypoxia: Characterization and Comparison With Hypoxia-Inducible Factor-1α
Wiesener, M. S., Turley, H., Allen, W. E., Willam, C., Eckardt, K., Talks, K., Wood, S. M., Gatter, K. C., Harris, A. L., Pugh, C. W., et al. (1998) · Blood · doi:10.1182/blood.v92.7.2260 · Cited by 629
Molecular characterization and chromosomal localization of a third alpha-class hypoxia inducible factor subunit, HIF3alpha.
Gu, Y.-Z., Moran, S. M., Hogenesch, J. B., Wartman, L. D., & Bradfield, C. A. (1998) · Europe PMC (PubMed Central) · Cited by 577
Alterations of expression of developmentally important genes in preimplantation bovine embryos by in vitro culture conditions: Implications for subsequent development
Niemann, H. J., & Wrenzycki, C. (2000) · Theriogenology · doi:10.1016/s0093-691x(99)00237-x · Cited by 475
Direct Comparison of GAPDH, β-Actin, Cyclophilin, and 28S rRNA as Internal Standards for Quantifying RNA Levels under Hypoxia
Zhong, H., & Simons, J. W. (1999) · Biochemical and Biophysical Research Communications · doi:10.1006/bbrc.1999.0815 · Cited by 473
The transcription factor EPAS-1/hypoxia-inducible factor 2α plays an important role in vascular remodeling
Peng, J., Zhang, L., Drysdale, L., & Fong, G.-H. (2000) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.140087397 · Cited by 460 · cited 1×
“5, developing severe vascular defects in both the yolk sac and embryo proper [32], suggesting that HIFs are regulators of critical developmental processes associated with normal postimplantation embryo development.”
Background
Cellular and Molecular Deviations in Bovine In Vitro-Produced Embryos Are Related to the Large Offspring Syndrome1
Lazzari, G., Wrenzycki, C., Herrmann, D., Duchi, R., Kruip, T. A. M., Niemann, H., & Galli, C. (2002) · Biology of Reproduction · doi:10.1095/biolreprod.102.004481 · Cited by 401
Protection against tissue damage in vivo by desferrioxamine: What is its mechanism of action?
Halliwell, B. B. (1989) · Free Radical Biology and Medicine · doi:10.1016/0891-5849(89)90145-7 · Cited by 378
Radical solutions and cultural problems: Could free oxygen radicals be responsible for the impaired development of preimplantation mammalian embryos in vitro?
Johnson, M. H., & Nasr‐Esfahani, M. H. (1994) · BioEssays · doi:10.1002/bies.950160105 · Cited by 342
Oxygen uptake and carbohydrate metabolism by in vitro derived bovine embryos
Thompson, J. G., Partridge, R. J., Houghton, F. D., Cox, C. I., & Leese, H. J. (1996) · Reproduction · doi:10.1530/jrf.0.1060299 · Cited by 333
Self-citation
REDOX regulation of early embryo development
Harvey, A. J., Kind, K. L., & Thompson, J. G. (2002) · Reproduction · doi:10.1530/rep.0.1230479 · Cited by 332
Self-citation
Effects of culture system and protein supplementation on mRNA expression in pre-implantation bovine embryos
Wrenzycki, C., Herrmann, D., Keskintepe, L., Martins, A., Sirisathien, S., Brackett, B. G., & Niemann, H. J. (2001) · Human Reproduction · doi:10.1093/humrep/16.5.893 · Cited by 328
Effect of oxygen concentration on in-vitro development of preimplantation sheep and cattle embryos
Thompson, J. G., Simpson, A. C., Pugh, P. A., Donnelly, P. E., & Tervit, H. R. (1990) · Reproduction · doi:10.1530/jrf.0.0890573 · Cited by 323
Self-citation
Cooperative Interaction of Hypoxia-inducible Factor-2α (HIF-2α) and Ets-1 in the Transcriptional Activation of Vascular Endothelial Growth Factor Receptor-2 (Flk-1)
Elvert, G., Kappel, A., Heidenreich, R., Englmeier, U., Lanz, S., Acker, T., Rauter, M., Plate, K. H., Sieweke, M. H., Breier, G., et al. (2003) · Journal of Biological Chemistry · doi:10.1074/jbc.m211298200 · Cited by 275
Role of Prolyl Hydroxylation in Oncogenically Stabilized Hypoxia-inducible Factor-1α
Chan, D. A., Sutphin, P. D., Denko, N. C., & Giaccia, A. J. (2002) · Journal of Biological Chemistry · doi:10.1074/jbc.m206922200 · Cited by 264
The hypoxia-inducible factors: key transcriptional regulators of hypoxic responses
Bracken, C. P., Whitelaw, M. L., & Peet, D. J. (2003) · Cellular and Molecular Life Sciences · doi:10.1007/s00018-003-2370-y · Cited by 259
Effects of oxygen toxicity on early development of mouse embryos
Umaoka, Y., Noda, Y., Narimoto, K., & Mori, T. (1992) · Molecular Reproduction and Development · doi:10.1002/mrd.1080310106 · Cited by 223
Granulocyte–macrophage colony-stimulating factor promotes human blastocyst development in vitro
Sjöblom, C., Wikland, M., & Robertson, S. A. (1999) · Human Reproduction · doi:10.1093/humrep/14.12.3069 · Cited by 209
Morphology and morphometry of in vivo- and in vitro-produced bovine concepti from early pregnancy to term and association with high birth weights
Bertolini, M., Mason, J. B., Beam, S. W., Carneiro, G. F., Sween, M. L., Kominek, D. J., Moyer, A. L., Famula, T. R., Sainz, R. D., & Anderson, G. B. (2002) · Theriogenology · doi:10.1016/s0093-691x(02)00935-4 · Cited by 204
Effect of inhibitors and uncouplers of oxidative phosphorylation during compaction and blastulation of bovine embryos cultured in vitro
Thompson, J. G., Mcnaughton, C., Gasparrini, B., McGowan, L., & Tervit, H. (2000) · Reproduction · doi:10.1530/reprod/118.1.47 · Cited by 192
Self-citation
Timing of Compaction and Inner Cell Allocation in Bovine Embryos Produced in Vivo after Superovulation1
Soom, A. V., Boerjan, M. L., Bols, P. E. J., Vanroose, G., Lein, A., Coryn, M., & de Kruif, A. (1997) · Biology of Reproduction · doi:10.1095/biolreprod57.5.1041 · Cited by 188
Hypoxic Regulation of Vascular Endothelial Growth Factor mRNA Stability Requires the Cooperation of Multiple RNA Elements
Dibbens, J. A., Miller, D. L., Damert, A., Risau, W., Vadas, M. A., & Goodall, G. J. (1999) · Molecular Biology of the Cell · doi:10.1091/mbc.10.4.907 · Cited by 185
The toxic effect of short exposures to the atmospheric oxygen concentration on early mouse embryonic development
Pabon, J. E., Findley, W. E., & Gibbons, W. E. (1989) · Fertility and Sterility · doi:10.1016/s0015-0282(16)60688-x · Cited by 178
Effects of oocyte quality, oxygen tension, embryo density, cumulus cells and energy substrates on cleavage and morula/blastocyst formation of bovine embryos
Khurana, N. K., & Niemann, H. J. (2000) · Theriogenology · doi:10.1016/s0093-691x(00)00387-3 · Cited by 168
Similarities and differences between the vascular endothelial growth factor (VEGF) splice variants
Neufeld, G., Cohen, T., Gitay-Goren, H., Gluzman‐Poltorak, Z., Tessler, S., Sharon, R., Gengrinovitch, S., & Levi, B.-Z. (1996) · Cancer and Metastasis Reviews · doi:10.1007/bf00437467 · Cited by 164
Oxygen concentration and protein source affect the development of preimplantation goat embryos in vitro
Batt, P. A., Gardner, D. K., & Cameron, A. (1991) · Reproduction Fertility and Development · doi:10.1071/rd9910601 · Cited by 151
Human assisted conception: a cautionary tale. Lessons from domestic animals
Leese, H. J., Donnay, I., & Thompson, J. G. (1998) · Human Reproduction · doi:10.1093/humrep/13.suppl_4.184 · Cited by 145
Self-citation
Hypoxia-inducible factor-1α is regulated at the post-mRNA level
Wenger, R. H., Kvietiko, I., Rolfs, A., Gassmann, M., & Marti, H. H. (1997) · Kidney International · doi:10.1038/ki.1997.79 · Cited by 139
Changes in the relative abundance of mRNA transcripts for insulin-like growth factor (IGF-I and IGF-II) ligands and their receptors (IGF-IR/IGF-IIR) in preimplantation bovine embryos derived from different in vitro systems
Yaseen, M., Wrenzycki, C., Herrmann, D., Carnwath, J., & Niemann, H. J. (2001) · Reproduction · doi:10.1530/rep.0.1220601 · Cited by 117
Expression of RNA from developmentally important genes in preimplantation bovine embryos produced in TCM supplemented with BSA
Wrenzycki, C., Herrmann, D., Carnwath, J. W., & Niemann, H. J. (1998) · Reproduction · doi:10.1530/jrf.0.1120387 · Cited by 109
Relationship Between Placental Vascular Endothelial Growth Factor Expression and Placental/Endometrial Vascularity in the Pig1
Vonnahme, K. A., Wilson, M. E., & Ford, S. P. (2001) · Biology of Reproduction · doi:10.1095/biolreprod64.6.1821 · Cited by 108
Cytokines induce HIF-1 DNA binding and the expression of HIF-1-dependent genes in cultured rat enterocytes
Scharte, M., Han, X., Bertges, D. J., Fink, M. P., & Delude, R. L. (2003) · American Journal of Physiology-Gastrointestinal and Liver Physiology · doi:10.1152/ajpgi.00076.2002 · Cited by 88
Vascular endothelial growth factor (VEGF) mRNA splice variants are differentially expressed in human blastocysts
Krüssel, J.-S. (2001) · Molecular Human Reproduction · doi:10.1093/molehr/7.1.57 · Cited by 76
Diabetes and Preimplantation Events of Embryogenesis
Moley, K. (1999) · Seminars in Reproductive Medicine · doi:10.1055/s-2007-1016221 · Cited by 69
An Unusual Subcellular Localization of GLUT1 and Link with Metabolism in Oocytes and Preimplantation Mouse Embryos1
Pantaleon, M., Ryan, J. P., Gil, M., & Kaye, P. L. (2001) · Biology of Reproduction · doi:10.1095/biolreprod64.4.1247 · Cited by 69
Self-citation
Development of one-cell ovine embryos in two culture media under two gas atmospheres
Betterbed, B., & Wright, R. W. (1985) · Theriogenology · doi:10.1016/0093-691x(85)90026-3 · Cited by 65
An investigation by mathematical modelling of whether mouse and human preimplantation embryos in static culture can satisfy their demands for oxygen by diffusion
Byatt‐Smith, J. G. B., Leese, H. J., & Gosden, R. G. (1991) · Human Reproduction · doi:10.1093/oxfordjournals.humrep.a137258 · Cited by 59
Isoform-Specific Expression of Hypoxia-Inducible Factor-1 During the Late Stages of Mouse Spermiogenesis
Marti, H. H., Katschinski, D. M., Wagner, K. F., Schäffer, L., Stier, B., & Wenger, R. H. (2002) · Molecular Endocrinology · doi:10.1210/mend.16.2.0786 · Cited by 55
Influence of culture system and oxygen tension on the development of ovine zygotes matured and fertilized in vitro
Bernardi, M. L., Fléchon, J. E., & Delouis, C. (1996) · Reproduction · doi:10.1530/jrf.0.1060161 · Cited by 51
Expression of vascular endothelial growth factor mRNA in human preimplantation embryos derived from tripronuclear zygotes
Krüssel, J.-S., Behr, B. R., Hirchenhain, J., Wen, Y., Milki, A. A., Cupisti, S., Bielfeld, P., & Polan, M. L. (2000) · Fertility and Sterility · doi:10.1016/s0015-0282(00)01581-8 · Cited by 43
Microvascular Endothelial Cells Differ in Basal and Hypoxia-Regulated Expression of Angiogenic Factors and Their Receptors
Tscheudschilsuren, G., Aust, G., Nieber, K., Schilling, N., & Spanel‐Borowski, K. (2002) · Microvascular Research · doi:10.1006/mvre.2001.2346 · Cited by 33
Down-regulation of Hypoxia-inducible Factor-2 in PC12 Cells by Nerve Growth Factor Stimulation
Naranjo‐Suarez, S., Castellanos, M. C., Álvarez-Tejado, M., Vara, A., Landázuri, M. O., & del Peso, L. (2003) · Journal of Biological Chemistry · doi:10.1074/jbc.m304079200 · Cited by 33
Beneficial effects of culturing rabbit zygotes to blastocysts in 5% oxygen and 10% carbon dioxide
Farrell, P. B., & Foote, R. H. (1995) · Reproduction · doi:10.1530/jrf.0.1030127 · Cited by 26
Molecular cloning of cDNAs encoding hypoxia-inducible factor (HIF)-1α and -2α of bovine arterial endothelial cells
Hara, S., Kobayashi, C., & Imura, N. (1999) · Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression · doi:10.1016/s0167-4781(99)00048-2 · Cited by 19
Incorporation of glucose by the sheep conceptus between days 13 and 19 of pregnancy
Wales, R. G., Cuneo, C. L., & Waugh, E. (1989) · Reproduction Fertility and Development · doi:10.1071/rd9890137 · Cited by 10
Expression of VEGF-system mRNAs in mouse blastocysts is not affected by in vitro culture
Ingmann, S. C., Hirchenhain, J., Huse, I., Hess, A. P., Bielfeld, P., & Krüssel, J.-S. (2002) · Fertility and Sterility · doi:10.1016/s0015-0282(02)03946-8 · Cited by 4
Oxidation of
Wales, R. G., & Waugh, E. (1993) · Reproduction Fertility and Development · doi:10.1071/rd9930201 · Cited by 4

Research works printed but not linked: 2

2003, Proceedings of the 34th annual conference of the Society for Reproductive Biology
2003, Proceedings of the 2nd world congress on fetal origins of adult disease

Sorted by citations

The role of oxygen availability in embryonic development and stem cell function
Simon, M. C., & Keith, B. (2008) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm2354 · Cited by 1,015
HIF-2α regulates Oct-4: effects of hypoxia on stem cell function, embryonic development, and tumor growthPreprint
Covello, K. L., Kehler, J., Yu, H., Gordan, J. D., Arsham, A. M., Hu, C., Labosky, P. A., Simon, M. C., & Keith, B. (2006) · Genes & Development · doi:10.1101/gad.1399906 · Cited by 850
Low O 2 tensions and the prevention of differentiation of hES cells
Ezashi, T., Das, P., & Roberts, R. M. (2005) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0501283102 · Cited by 824
Placental Origins of Chronic Disease
Burton, G. J., Fowden, A. L., & Thornburg, K. L. (2016) · Physiological Reviews · doi:10.1152/physrev.00029.2015 · Cited by 717
Mitochondrial Regulation of Cell Cycle and Proliferation
Arciuch, V. G. A., Elguero, M. E., Poderoso, J. J., & Carreras, M. C. (2011) · Antioxidants and Redox Signaling · doi:10.1089/ars.2011.4085 · Cited by 449

See all 189

Authors: Harvey, A. J., Kind, K. L., Pantaleon, M., Armstrong, D. T., & Thompson, J. G.
Affiliations: Queen Elizabeth Hospital (AU)
The University of Queensland (AU)
Corresponding author: Harvey, A.J.
ORCID: Harvey, A.J.
Kind, K.L.
Pantaleon, M.
Thompson, J.G.
Venue: Biology of Reproduction
Publisher: Oxford University Press (OUP)
Published: 2004-10-01
Volume: 71
Issue: 4
Pages: 1108-1119
ISSN: 0006-3363 (ISSN-L)
1529-7268
DOI: 10.1095/biolreprod.104.028639
OpenAlex: W2154875545
Cited by: 189
References: 66
Citations by year: 2026: 1 · 2025: 4 · 2024: 4 · 2023: 2 · 2022: 14 · 2021: 6 · 2020: 15 · 2019: 4 · 2018: 4 · 2017: 10 · 2016: 6 · 2015: 13 · 2014: 4 · 2013: 13 · 2012: 9
FWCI: 12.56
Type: journal article
Language: en
Open access: closed
Locations: Biology of Reproduction (publishedVersion)
PubMed (publishedVersion)
BioOne Complete (BioOne) (submittedVersion)
Adelaide Research & Scholarship (AR&S) (University of Adelaide) (submittedVersion)
Funders: Medical Research Council
National Health and Medical Research Council (157941)
Topics: Health Sciences › Medicine › Reproductive Medicine › Reproductive Biology and Fertility
Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › Cancer, Hypoxia, and Metabolism
Life Sciences › Agricultural and Biological Sciences › Animal Science and Zoology › Reproductive Physiology in Livestock
Keywords: oxygen regulation, bovine blastocyst, hypoxia-inducible factor, HIF-1α, HIF-2α, GLUT1, embryo culture, oxygen partial pressure, deferoxamine, inner cell mass, bHLH transcription factors, embryonic development, cumulus cells
MeSH terms: Endothelial PAS Domain-Containing Protein 1, Animals, Blastocyst, Cattle, Monosaccharide Transport Proteins, Oxygen, RNA, Messenger, Tissue Distribution, Transcription Factors, Gene Expression Regulation, Developmental, Vascular Endothelial Growth Factor A, Embryo Culture Techniques, Embryonic Development, Glucose Transporter Type 1, Basic Helix-Loop-Helix Proteins, Hypoxia-Inducible Factor 1, alpha Subunit

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review

Oxygen-Regulated Gene Expression in Bovine Blastocysts1

Abstract

Overview

Integrity signals

Verification

Claims the paper makes

Claims other papers make about this one

Citations

Cites

Cited by

Related

Metadata

Record

About these checks