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Differential Expression of Estrogen Receptor-α and -β and Androgen Receptor in the Ovaries of Marmosets and Humans
Abstract
Estrogens and androgens are essential for the maturation of the ovarian follicle and normal fertility in the female. We have used antibodies specific for both forms of estrogen receptor (alpha [ERalpha] and beta [ERbeta]) and androgen receptor (AR) to investigate the pattern of receptor expression in ovaries obtained from women and from a New World primate, the Common marmoset (Callthrix jacchus). On Western blots, three antibodies directed against different peptides within human ERbeta all recognized recombinant human (h) ERbeta but did not bind to recombinant hERalpha. The ERbeta protein was extracted from human ovary and prostate and marmoset ovary. In marmoset and human ovaries, ERbeta protein was detected in the nuclei of granulosa cells in all sizes of follicle (both before and after formation of the antrum), and it was also detected in thecal cells, corpora lutea, surface epithelium, and stroma. In contrast, ERalpha protein was not detected in the nuclei of granulosa cells in preantral follicles, was low/absent from stromal and thecal cells, but was expressed in granulosa cells of antral follicles and in the surface epithelium. The pattern of expression of AR protein more closely resembled that of ERbeta than ERalpha. In conclusion, three independent antibodies have demonstrated convincingly that ERbeta is expressed in a wide range of cells in the primate ovary. Granulosa cells in preantral follicles could contain ERbeta:beta dimers. In antral follicles, however, ERalpha is also expressed, and the formation of homo- or heterodimers containing ERalpha may influence the pattern of gene activation within these cells.
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“Detailed immunohistochemical studies using fixed full-thickness endometrial tissues in combination with validated antibodies [27] have identified nuclear staining for AR, ERalpha and ERbeta in the human endometrium during the normal menstrual cycle [16,18,28,29].”
From Endometrial Intracrinology: Oestrogens, Androgens and Endometrial Disorders (2018) · cites this paper as [27]
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Metadata
- Authors
- Saunders, P. T. K., Millar, M. R., Williams, K., Macpherson, S., Harkiss, D., Anderson, R. A., Orr, B., Groome, N. P., Scobie, G., & Fraser, H. M.
- Affiliations
- Oxford Brookes University (GB)
- Corresponding author
- Saunders, Philippa T.K.
- Venue
- Biology of Reproduction
- Publisher
- Oxford University Press (OUP)
- Published
- 2000-10-01
- Volume
- 63
- Issue
- 4
- Pages
- 1098-1105
- ISSN
- 0006-3363 (ISSN-L)
- 1529-7268
- OpenAlex
- W1971365457
- Cited by
- 175
- References
- 49
- Citations by year
- 2025: 2 · 2024: 4 · 2023: 6 · 2022: 5 · 2021: 3 · 2020: 3 · 2019: 2 · 2018: 4 · 2017: 2 · 2016: 6 · 2015: 6 · 2014: 6 · 2013: 2 · 2012: 2
- FWCI
- 9.36
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Biology of Reproduction (publishedVersion)
- PubMed (publishedVersion)
- BioOne Complete (BioOne) (submittedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Endocrinology › Estrogen and related hormone effects
- Health Sciences › Medicine › Reproductive Medicine › Reproductive Biology and Fertility
- Health Sciences › Medicine › Reproductive Medicine › Ovarian function and disorders
- Keywords
- estrogen receptor alpha, estrogen receptor beta, androgen receptor, marmoset, Callithrix jacchus, human ovary, granulosa cells, theca cells, corpus luteum, ovarian follicle maturation, receptor heterodimers, antibody specificity
- MeSH terms
- Amino Acid Sequence, Animals, Antibody Specificity, Callithrix, Female, Gene Expression Regulation, Ovarian Follicle, Humans, Molecular Sequence Data, Ovary, Receptors, Androgen, Receptors, Estrogen, Transcriptional Activation, Estrogen Receptor alpha, Estrogen Receptor beta
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