ERβ: Identification and characterization of a novel human estrogen receptor
Open accessAbstract
A novel estrogen receptor (hereinafter referred to as ERβ) was cloned using degenerate PCR primers. A comparison of the amino acid sequence of ERβ with the ‘classical’ ER (ERα) shows a high degree of conservation of the DNA‐binding domain (96%), and of the ligand‐binding domain (58%). In contrast, the A/B domain, the hinge region and the F‐domain are not conserved. Northern blot analysis revealed that ERβ is expressed in human thymus, spleen, ovary and testis. Transient transfections of an ERβ expression construct together with an ERE‐based reporter construct in CHO cells clearly demonstrated transactivation of ERβ by 17β‐estradiol. In addition, the ERα antagonist ICI‐164384 is a potent antagonist for ERβ as well. Interestingly, the level of transactivation by 17β‐estradiol is higher for ERα than for ERβ, which may reflect suboptimal conditions for ERβ at the level of the ligand, responsive element or cellular context.
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- Authors
- Mosselman, S., Polman, J., & Dijkema, R.
- Affiliations
- Organon (Netherlands) (NL)
- Corresponding author
- Mosselman, Sietse
- Venue
- FEBS Letters
- Publisher
- Wiley
- Published
- 1996-08-19
- Volume
- 392
- Issue
- 1
- Pages
- 49-53
- ISSN
- 0014-5793 (ISSN-L)
- 1873-3468
- OpenAlex
- W2161623422
- Cited by
- 2,269
- References
- 15
- Citations by year
- 2026: 8 · 2025: 19 · 2024: 12 · 2023: 12 · 2022: 30 · 2021: 44 · 2020: 32 · 2019: 39 · 2018: 40 · 2017: 41 · 2016: 38 · 2015: 45 · 2014: 42 · 2013: 53 · 2012: 66
- FWCI
- 66.18
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- FEBS Letters (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Estrogen and related hormone effects
- Physical Sciences › Environmental Science › Health, Toxicology and Mutagenesis › Effects and risks of endocrine disrupting chemicals
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Receptor Mechanisms and Signaling
- Keywords
- estrogen receptor beta, estrogen receptor alpha, gene cloning, DNA-binding domain, ligand-binding domain, tissue expression, antiestrogens, estrogen response element
- MeSH terms
- Amino Acid Sequence, Base Sequence, Cell Line, Estradiol, Estrogen Antagonists, Female, Gene Expression Regulation, Humans, Male, Molecular Sequence Data, Ovary, Receptors, Estrogen, Testis, Thymus Gland, Sequence Homology, Amino Acid, Genes, Reporter, DNA, Complementary, Estrogen Receptor beta, Polyunsaturated Alkamides
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