The DOI matches its Crossref record; 50 of the 50 references it cites were found in a registry or an index.
Triiodothyronine‐mediated upregulation of UCP2 and UCP3 mRNA expression in human skeletal muscle without coordinated induction of mitochondrial respiratory chain genes
Abstract
Triiodothyronine (T3) increases mitochondrial respiration and promotes the uncoupling between oxygen consumption and ATP synthesis. T3 effect is mediated partly through transcriptional control of genes encoding mitochondrial proteins. We determined the effect of T3 on mRNA levels of uncoupling proteins (UCP) and proteins involved in the biogenesis of the respiratory chain in human skeletal muscle and on UCP2 mRNA expression in adipose tissue. Ten young, healthy males received 75 to 100 µg of T3 per day for 14 days. The increase in plasma‐free T3 levels was associated with an increase of resting metabolic rate and a decrease of respiratory quotient. In skeletal muscle, treatment with T3 induced a twofold increase of both UCP2 and UCP3 mRNA levels (p < 0.01). The mRNA levels of cytochrome c oxidase subunits 2 and 4, nuclear respiratory factor 1, mitochondrial transcription factor A, and the co‐activator PGC1 did not change during the treatment. In adipose tissue, UCP2 mRNA levels increased threefold. The direct effect of T3 on skeletal muscle and adipose tissue UCP2 and UCP3 mRNA expression was demonstrated in vitro in human primary cultures. Our data show that T3 induces UCP2 and UCP3 mRNA expression in humans. In skeletal muscle, UCP regulation by T3 is not associated with the transcriptional regulation of respiratory chain proteins.
Overview
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
In the citing paper
“In addition, most utilized high doses of levotriiodothyronine (LT3), not minimally suppressive doses of LT4.”
From Effects of levothyroxine replacement or suppressive therapy on energy expenditure and body composition (2016) · cites this paper as [4]
In the citing paper
“These studies uniformly showed increased resting and 24-hour energy expenditure, but were too brief to document changes in body composition.”
From Effects of levothyroxine replacement or suppressive therapy on energy expenditure and body composition (2016) · cites this paper as [4]
In the citing paper
“Past studies have reported increased resting or 24-hour energy expenditure and whole body oxygen consumption with exogenous administration of pharmacologic doses of LT4 or LT3 to healthy humans (3-7) due to increased expression of uncoupling proteins and decreased mitochondrial efficiency (3,4,7).”
From Effects of levothyroxine replacement or suppressive therapy on energy expenditure and body composition (2016) · cites this paper as [4]
Citations
Cites
Wu, Z., Puigserver, P., Andersson, U., Zhang, C., Adelmant, G., Mootha, V., Troy, A., Cinti, S., Lowell, B., Scarpulla, R. C., et al. (1999) · Cell · doi:10.1016/s0092-8674(00)80611-x · Cited by 4,139
A Cold-Inducible Coactivator of Nuclear Receptors Linked to Adaptive Thermogenesis
Puigserver, P., Wu, Z., Park, C. W., Graves, R., Wright, M., & Spiegelman, B. M. (1998) · Cell · doi:10.1016/s0092-8674(00)81410-5 · Cited by 3,864
Uncoupling protein-2: a novel gene linked to obesity and hyperinsulinemia
Fleury, C., Neverova, M., Collins, S., Raimbault, S., Champigny, O., Lévi-Meyrueis, C., Bouillaud, F., Seldin, M. F., Surwit, R. S., Ricquier, D., et al. (1997) · Nature Genetics · doi:10.1038/ng0397-269 · Cited by 1,630
Boss, O., Samec, S., Paoloni‐Giacobino, A., Rossier, C., Dulloo, A. G., Seydoux, J., Muzzin, P., & Giacobino, J. (1997) · FEBS Letters · doi:10.1016/s0014-5793(97)00384-0 · Cited by 1,041
The uncoupling protein homologues: UCP1, UCP2, UCP3, StUCP and AtUCP
Ricquier, D., & Bouillaud, F. (2000) · Biochemical Journal · doi:10.1042/bj3450161 · Cited by 811
A role for uncoupling protein‐2 as a regulator of mitochondrial hydrogen peroxide generation
Nègre‐Salvayre, A., Hirtz, C., Carrera, G., Cazenave, R., Troly, M., Salvayre, R., Pénicaud, L., & Casteilla, L. (1997) · The FASEB Journal · doi:10.1096/fasebj.11.10.9271366 · Cited by 773
Gong, D., He, Y., Karas, M., & Reitman, M. L. (1997) · Journal of Biological Chemistry · doi:10.1074/jbc.272.39.24129 · Cited by 740
Vidal‐Puig, A., Solanes, G., Grujić, D., Flier, J. S., & Lowell, B. B. (1997) · Biochemical and Biophysical Research Communications · doi:10.1006/bbrc.1997.6740 · Cited by 711
Energy metabolism in uncoupling protein 3 gene knockout mice
Vidal-Puig, A. J., Grujic, D., Zhang, C.-Y., Hagen, T., Boss, O., Ido, Y., Szczepanik, A., Wade, J., Mootha, V., Cortright, R., et al. (2000) · Journal of Biological Chemistry · doi:10.1074/jbc.m910179199 · Cited by 677
Mice overexpressing human uncoupling protein-3 in skeletal muscle are hyperphagic and lean
Clapham, J. C., Arch, J. R. S., Chapman, H., Haynes, A., Lister, C., Moore, G. B. T., Piercy, V., Carter, S. A., Lehner, I., Smith, S. A., et al. (2000) · Nature · doi:10.1038/35019082 · Cited by 604
Research works printed but not linked: 2
- Arner P., 1996, Regulation of fat cell lipolysis, Diabetes Rev., 4, 450
- 10.1210/en.136.3.1003 · DOI
Cited by
Sorted by citations
Thyroid Hormone Regulation of Metabolism
Mullur, R., Liu, Y.-Y., & Brent, G. A. (2014) · Physiological Reviews · doi:10.1152/physrev.00030.2013 · Cited by 2,421
Thermogenic Mechanisms and Their Hormonal Regulation
Silva, J. E. (2006) · Physiological Reviews · doi:10.1152/physrev.00009.2005 · Cited by 687
Duan, Y., Gong, K., Xu, S., Zhang, F., Meng, X., & Han, J. (2022) · Signal Transduction and Targeted Therapy · doi:10.1038/s41392-022-01125-5 · Cited by 538
Hypothalamus‐Pituitary‐Thyroid Axis
Ortiga-Carvalho, T. M., Chiamolera, M. I., Pazos-Moura, C. C., & Wondisford, F. E. (2016) · Comprehensive physiology · doi:10.1002/cphy.c150027 · Cited by 485
Role of thyroid hormone in skeletal muscle physiology
Bloise, F. F., Cordeiro, A., & Ortiga-Carvalho, T. M. (2017) · Journal of Endocrinology · doi:10.1530/joe-16-0611 · Cited by 242
Metadata
- Authors
- Barbe, P., Larrouy, D., Boulanger, C., Chevillotte, E., Viguerie, N., Thalamas, C., Oliva Trastoy, M., Roques, M., Vidal, H., & Langin, D.
- Affiliations
- Université Toulouse III - Paul Sabatier (FR)
- Inserm (FR)
- Hôpital Purpan (FR)
- INSERM Unit 317 Institut Louis Bugnard Université Paul Sabatier Hôpital Rangueil Toulouse France
- ORCID
- Venue
- The FASEB Journal
- Publisher
- Wiley
- Published
- 2000-11-09
- Volume
- 15
- Issue
- 1
- Pages
- 13-15
- ISSN
- 0892-6638 (ISSN-L)
- 1530-6860
- OpenAlex
- W2159785275
- Cited by
- 118
- References
- 49
- Citations by year
- 2026: 2 · 2025: 3 · 2024: 1 · 2023: 2 · 2022: 6 · 2021: 3 · 2020: 1 · 2019: 3 · 2018: 4 · 2017: 3 · 2016: 7 · 2015: 4 · 2014: 2 · 2013: 2 · 2012: 7
- FWCI
- 2.65
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- The FASEB Journal (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Institut National de la Santé et de la Recherche Médicale (4P007E, 4P020D)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Mitochondrial Function and Pathology
- Health Sciences › Medicine › Physiology (medical) › Adipose Tissue and Metabolism
- Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Thyroid Disorders and Treatments
- Keywords
- triiodothyronine, uncoupling proteins, UCP2, UCP3, skeletal muscle, adipose tissue, mitochondrial uncoupling, mitochondrial transcription factors, PGC-1, resting metabolic rate, respiratory quotient
- MeSH terms
- Uncoupling Protein 2, Uncoupling Protein 3, Adipose Tissue, Carrier Proteins, Cells, Cultured, DNA, Mitochondrial, Electron Transport, Humans, Ion Channels, Male, Mitochondria, Models, Biological, Models, Genetic, Proteins, RNA, Messenger, Triiodothyronine, Uncoupling Agents, Up-Regulation, Muscle, Skeletal, Mitochondrial Proteins, Membrane Transport Proteins
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
