The DOI matches its Crossref record; 47 of the 47 references it cites were found in a registry or an index.
Stem cell differentiation requires a paracrine pathway in the heart
Abstract
Members of the transforming growth factor pβ (TGF‐β) superfamily‐namely, TGF‐β and BMP2—applied to undifferentiated murine embryonic stem cells up‐regulated mRNA of mesodermal (Brachyury) and cardiac specific transcription factors (Nkx2.5, MEF2C). Embryoid bodies generated from stem cells primed with these growth factors demonstrated an increased potential for cardiac differentiation with a significant increase in beating areas and enhanced myofibrillogenesis. In an environment of postmitotic cardiomyocytes, stem cells engineered to express a fluorescent protein under the control of a cardiac promoter differentiated into fluorescent ventricular myocytes beating in synchrony with host cells, a process significantly enhanced by TGF‐β or BMP2. In vitro, disruption of the TGF‐β/BMP signaling pathways by latency‐associated peptide and/or noggin prevented differentiation of stem cells. In fact, only host cells that secrete a TGF‐β family member induced a cardiac phenotype in stem cells. In vivo, transplantation of stem cells into heart also resulted in cardiac differentiation provided that TGF‐β/BMP2 signaling was intact. In infarcted myocardium, grafted stem cells differentiated into functional cardiomyocytes integrated with surrounding tissue, improving contractile performance. Thus, embryonic stem cells are directed to differentiate into cardiomyocytes by signaling mediated through TGF‐β/BMP2, a cardiac paracrine pathway required for therapeutic benefit of stem cell transplantation in diseased heart.—Behfar, A., Zingman, L. V., Hodgson, D. M., Rauzier, J.‐M., Kane, G. C., Terzic, A., Pucéat, M. Stem cell differentiation requires a paracrine pathway in the heart. FASEB J . 16, 1558–1566 (2002)
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction
Chomczynski, P., & Sacchi, N. (1987) · Analytical Biochemistry · doi:10.1016/0003-2697(87)90021-2 · Cited by 46,426
Bone marrow cells regenerate infarcted myocardium
Orlic, D., Kajstura, J., Chimenti, S., Jakoniuk, I., Anderson, S. M., Li, B., Pickel, J., McKay, R., Nadal-Ginard, B., Bodine, D. M., et al. (2001) · Nature · doi:10.1038/35070587 · Cited by 5,155
Purified hematopoietic stem cells can differentiate into hepatocytes in vivo
Lagasse, E., Connors, H., Al-Dhalimy, M., Reitsma, M., Dohse, M., Osborne, L., Wang, X., Finegold, M., Weissman, I. L., & Grompe, M. (2000) · Nature Medicine · doi:10.1038/81326 · Cited by 2,314
Bone marrow cells adopt the phenotype of other cells by spontaneous cell fusion
Terada, N., Hamazaki, T., Oka, M., Hoki, M., Mastalerz, D. M., Nakano, Y., Meyer, E. M., Morel, L., Petersen, B. E., & Scott, E. W. (2002) · Nature · doi:10.1038/nature730 · Cited by 1,999
Regeneration of ischemic cardiac muscle and vascular endothelium by adult stem cells
Jackson, K. A., Majka, S. M., Wang, H., Pocius, J., Hartley, C. J., Majesky, M. W., Entman, M. L., Michael, L. H., Hirschi, K. K., & Goodell, M. A. (2001) · Journal of Clinical Investigation · doi:10.1172/jci12150 · Cited by 1,960
From Marrow to Brain: Expression of Neuronal Phenotypes in Adult Mice
Brazelton, T. R., Rossi, F. M. V., Keshet, G. I., & Blau, H. M. (2000) · Science · doi:10.1126/science.290.5497.1775 · Cited by 1,676
The Spemann Organizer Signal noggin Binds and Inactivates Bone Morphogenetic Protein 4
Zimmerman, L. B., De Jesús-Escobar, J. M., & Harland, R. M. (1996) · Cell · doi:10.1016/s0092-8674(00)80133-6 · Cited by 1,633
Changing potency by spontaneous fusion
Ying, Q.-L., Nichols, J., Evans, E. P., & Smith, A. G. (2002) · Nature · doi:10.1038/nature729 · Cited by 1,494
Evidence That Human Cardiac Myocytes Divide after Myocardial Infarction
Beltrami, A. P., Urbanek, K., Kajstura, J., Yan, S.-M., Finato, N., Bussani, R., Nadal-Ginard, B., Silvestri, F., Leri, A., Beltrami, C. A., et al. (2001) · New England Journal of Medicine · doi:10.1056/nejm200106073442303 · Cited by 1,429
McDonald, J. W., Liu, X.-Z., Qu, Y., Liu, S., Mickey, S. K., Turetsky, D., Gottlieb, D. I., & Choi, D. W. (1999) · Nature Medicine · doi:10.1038/70986 · Cited by 1,226
Cited by
Laflamme, M. A., & Murry, C. E. (2005) · Nature Biotechnology · doi:10.1038/nbt1117 · Cited by 964
Unchain my heart: the scientific foundations of cardiac repair
Dimmeler, S., Zeiher, A. M., & Schneider, M. D. (2005) · Journal of Clinical Investigation · doi:10.1172/jci200524283 · Cited by 668
Cao, F., Lin, S., Xie, X., Ray, P., Patel, M., Zhang, X., Drukker, M., Dylla, S. J., Connolly, A. J., Chen, X., et al. (2006) · Circulation · doi:10.1161/circulationaha.105.588954 · Cited by 493
Cardiac Stem Cells and Mechanisms of Myocardial Regeneration
Leri, A., Kajstura, J., & Anversa, P. (2005) · Physiological Reviews · doi:10.1152/physrev.00013.2005 · Cited by 451
Formation of Human Myocardium in the Rat Heart from Human Embryonic Stem Cells
Laflamme, M. A., Gold, J., Xu, C., Hassanipour, M., Rosler, E., Police, S., Muskheli, V., & Murry, C. E. (2005) · The American Journal of Pathology · doi:10.1016/s0002-9440(10)62041-x · Cited by 448
Metadata
- Authors
- Behfar, A., Zingman, L. V., Hodgson, D. M., Rauzier, J., Kane, G. C., Terzic, A., & Pucéat, M.
- Venue
- The FASEB Journal
- Publisher
- Wiley
- Published
- 2002-10
- Volume
- 16
- Issue
- 12
- Pages
- 1558-1566
- ISSN
- 0892-6638 (ISSN-L)
- 1530-6860
- OpenAlex
- W1979608640
- Cited by
- 477
- References
- 47
- FWCI
- 8.42
- Type
- journal article
- Language
- en
- Open access
- closed
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: Stem cell differentiation requires a paracrine pathway in the heart. https://app.cochat.ai/papers/10.1096/fj.02-0072com
- The paper
- Behfar, A., Zingman, L. V., Hodgson, D. M., Rauzier, J., Kane, G. C., Terzic, A., & Pucéat, M. (2002). Stem cell differentiation requires a paracrine pathway in the heart. The FASEB Journal. https://doi.org/10.1096/fj.02-0072com
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
