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Highly efficient cellular labeling of mesoporous nanoparticles in human mesenchymal stem cells: implication for stem cell tracking
Abstract
Tracking the distribution of stem cells is crucial to their therapeutic use. However, the usage of current vectors in cellular labeling is restricted by their low internalizing efficiency. Here, we reported a cellular labeling approach with a novel vector composed of mesoporous silica nanoparticles (MSNs) conjugated with fluorescein isothiocyanate in human bone marrow mesenchymal stem cells and 3T3‐L1 cells, and the mechanism about fluorescein isothiocyanate‐conjugated MSNs (FITC‐MSNs) internalization was studied. FITC‐MSNs were efficiently internalized into mesenchymal stem cells and 3T3‐L1 cells even in short‐term incubation. The process displayed a time‐ and concentration‐dependent manner and was dependent on clathrin‐mediated endocytosis. In addition, clathrin‐dependent endocytosis seemed to play a decisive role on more internalization and longer stay of FITC‐MSNs in mesenchymal stem cells than in 3T3‐L1 cells. The internalization of FITC‐MSNs did not affect the cell viability, proliferation, immunophenotype, and differentiation potential of mesenchymal stem cells, and 3T3‐L1 cells. Finally, FITC‐MSNs could escape from endolysosomal vesicles and were retained the architectonic integrity after internalization. We conclude that the advantages of biocompatibility, durability, and higher efficiency in internalization suit MSNs to be a better vector for stem cell tracking than others currently used.
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Cited by
Mesoporous silica nanoparticles for drug delivery and biosensing applications
Slowing, I. I., Trewyn, B. G., Giri, S., & Lin, V. S. ‐. (2007) · Advanced Functional Materials · doi:10.1002/adfm.200601191 · Cited by 1,699
Trewyn, B. G., Slowing, I. I., Giri, S., Chen, H.-T., & Lin, V. S.-Y. (2007) · Accounts of Chemical Research · doi:10.1021/ar600032u · Cited by 1,123
Harush-Frenkel, O., Rozentur, E., Benita, S., & Altschuler, Y. (2008) · Biomacromolecules · doi:10.1021/bm700535p · Cited by 405
Surface charge-mediated rapid hepatobiliary excretion of mesoporous silica nanoparticles
Souris, J. S., Lee, C.-H., Cheng, S.-H., Chen, C.-T., Yang, C.-S., Ho, J.-A. A., Mou, C.-Y., & Lo, L.-W. (2010) · Biomaterials · doi:10.1016/j.biomaterials.2010.03.048 · Cited by 320
Self-citationModels for oral uptake of nanoparticles in consumer products
Fröhlich, E., & Roblegg, E. (2012) · Toxicology · doi:10.1016/j.tox.2011.11.004 · Cited by 295
Metadata
- Authors
- Huang, D., Hung, Y., Ko, B., Hsu, S., Chen, W., Chien, C., Tsai, C., Kuo, C., Kang, J., Yang, C., Mou, C., & Chen, Y.
- Venue
- The FASEB Journal
- Publisher
- Wiley
- Published
- 2005-12
- Volume
- 19
- Issue
- 14
- Pages
- 2014-2016
- ISSN
- 0892-6638 (ISSN-L)
- 1530-6860
- OpenAlex
- W2171040892
- Cited by
- 289
- References
- 0
- FWCI
- 4.17
- Type
- journal article
- Language
- en
- Open access
- closed
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- Huang, D., Hung, Y., Ko, B., Hsu, S., Chen, W., Chien, C., Tsai, C., Kuo, C., Kang, J., Yang, C., Mou, C., & Chen, Y. (2005). Highly efficient cellular labeling of mesoporous nanoparticles in human mesenchymal stem cells: implication for stem cell tracking. The FASEB Journal. https://doi.org/10.1096/fj.05-4288fje
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