Cited by 611Gut microbiota and healthAdipokines, Inflammation, and Metabolic DiseasesDiet, Metabolism, and Disease

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Germ‐free C57BL/6J mice are resistant to high‐fat‐diet‐induced insulin resistance and have altered cholesterol metabolism

Rabot, Sylvie, Membrez, Mathieu, Bruneau, Aurélia, Gérard, Philippe, Harach, Taoufiq, Moser, Mireille, Raymond, Frederic, Mansourian, Robert, Chou, Chieh J.·2010·The FASEB Journal

DOI 10.1096/fj.10.164921

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Abstract

Recent studies showed that germ‐free (GF) mice are resistant to obesity when consuming a high‐fat, high‐carbohydrate Western diet. However, it remains unclear what mechanisms are involved in the antiobesity phenotype and whether GF mice develop insulin resistance and dyslipidemia with high‐fat (HF) feeding. In the present study, we compared the metabolic consequences of HF feeding on GF and conventional (conv) C57BL/6J mice. GFmice consumed fewer calories, excreted more fecal lipids, and weighed significantly less than conv mice. GF/HF animals also showed enhanced insulin sensitivity with improved glucose tolerance, reduced fasting and nonfasting insuline‐mia, and increased phospho‐Akt (Ser‐473) in adipose tissue. In association with enhanced insulin sensitivity, GF/HF mice had reduced plasma TNF‐α and total serum amyloid A concentrations. Reduced hypercholes‐terolemia, a moderate accretion of hepatic cholesterol, and an increase in fecal cholesterol excretion suggest an altered cholesterol metabolism in GF/HF mice. Pronounced nucleus SREBP2 proteins and up‐regulation of cholesterol biosynthesis genes indicate that enhanced cholesterol biosynthesis contributed to the cholesterol homeostasis in GF/HF mice. Our results demonstrate that fewer calorie consumption and increased lipid excretion contributed to the obesity‐resistant phenotype of GF/HF mice and reveal that insulin sensitivity and cholesterol metabolism are metabolic targets influenced by the gut microbiota.— Rabot, S., Membrez, M., Bruneau, A., Gerard, P., Harach, T., Moser, M., Raymond, F., Mansourian, R., Chou. C. J. Germ‐free C57BL/6J mice are resistant to high‐fat‐diet‐induced insulin resistance and have altered cholesterol metabolism. FASEB J. 24, 4948–4959 (2010). www.fasebj.org

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Result
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Background
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Background
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Background
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Self-citation
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Background
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Background
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“Later, it was further shown that GF mice receiving a HFD showed enhanced insulin sensitivity with improved glucose tolerance and reduced insulinaemia (90).”
BackgroundSelf-citation
The effects of amoxicillin and vancomycin on parameters reflecting cholesterol metabolism
Baumgartner, S., Reijnders, D., Konings, M. C. J. M., Groen, A. K., Lütjohann, D., Goossens, G. H., Blaak, E. E., & Plat, J. (2017) · Chemistry and Physics of Lipids · doi:10.1016/j.chemphyslip.2017.06.006 · Cited by 12
“Compared to conventionally raised mice on a chow diet, germ-free mice had comparable plasma high-density lipoprotein cholesterol (HDL-C) and lower serum low-density lipoprotein cholesterol (LDL-C) concentrations (Velagapudi et al., 2010), while germ-free mice on a high-fat diet had lower serum HDL-C and total choleste…”
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“In the gastrointestinal tract of mice with lipid metabolism disorder, the number of Scleroderma and Bacteroides was significantly increased compared with that of the normal host.([49,50]) R.”
Background
Metabolic alterations of the gut–liver axis induced by cholic acid contribute to hepatic steatosis in rats
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Background

Authors: Rabot, S., Membrez, M., Bruneau, A., Gérard, P., Harach, T., Moser, M., Raymond, F., Mansourian, R., & Chou, C. J.
Affiliations: Microbiologie de l’alimentation au service de la santé (FR)
Department of Nutrition and Health Nestlé Research Center Lausanne Switzerland
Nestlé (Switzerland) (CH)
Department of Bioanalytical Science Nestlé Research Center Lausanne Switzerland
ORCID: Rabot, Sylvie
Membrez, Mathieu
Bruneau, Aurélia
Gérard, Philippe
Moser, Mireille
Chou, Chieh J.
Venue: The FASEB Journal
Publisher: Wiley
Published: 2010-08-19
Volume: 24
Issue: 12
Pages: 4948-4959
ISSN: 0892-6638 (ISSN-L)
1530-6860
DOI: 10.1096/fj.10.164921
OpenAlex: W2088697466
Cited by: 610
References: 41
Citations by year: 2026: 9 · 2025: 19 · 2024: 34 · 2023: 43 · 2022: 50 · 2021: 64 · 2020: 75 · 2019: 49 · 2018: 42 · 2017: 40 · 2016: 39 · 2015: 44 · 2014: 24 · 2013: 33 · 2012: 22
FWCI: 10.17
Type: journal article
Language: en
Open access: closed
Licence: onlinelibrary.wiley.com/termsAndConditions#vor (vor)
Locations: The FASEB Journal (publishedVersion)
PubMed (publishedVersion)
HAL (submittedVersion)
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gut microbiota and health
Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Adipokines, Inflammation, and Metabolic Diseases
Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Diet, Metabolism, and Disease
Keywords: germ-free mice, gut microbiota, high-fat diet, insulin resistance, cholesterol metabolism, hypercholesterolemia, obesity resistance, glucose tolerance, TNF-α, serum amyloid A, SREBP2, cholesterol biosynthesis, liver cholesterol, fecal cholesterol excretion, C57BL/6J mice
MeSH terms: Animals, Body Weight, Cholesterol, Dietary Fats, Germ-Free Life, Glucose Tolerance Test, Glycogen, Insulin Resistance, Lipids, Liver, Male, Mice, Inbred C57BL, Obesity, Blotting, Western, Polymerase Chain Reaction, Oligonucleotide Array Sequence Analysis, Mice

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Germ‐free C57BL/6J mice are resistant to high‐fat‐diet‐induced insulin resistance and have altered cholesterol metabolism

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