Cited by 55Cellular transport and secretionNeuroscience and Neuropharmacology ResearchExtracellular vesicles in disease

Chromogranins A and B are key proteins in amine accumulation, but the catecholamine secretory pathway is conserved without them

Díaz‐Vera, Jésica, Camacho, Marcial, Machado, José David, Domínguez, Natalia, Montesinos, Mónica S., Hernández‐Fernaud, Juan R., Luján, Rafael, Borges, Ricardo·2012·The FASEB Journal

Abstract

ABSTRACT Chromogranins are the main soluble proteins in the large dense core secretory vesicles (LDCVs) found in aminergic neurons and chromaffin cells. We recently demonstrated that chromogranins A and B each regulate the concentration of adrenaline in chromaffin granules and its exocytosis. Here we have further studied the role played by these proteins by generating mice lacking both chromogranins. Surprisingly, these animals are both viable and fertile. Although chromogranins are thought to be essential for their biogenesis, LDCVs were evident in these mice. These vesicles do have a somewhat atypical appearance and larger size. Despite their increased size, single‐cell amperometry recordings from chromaffin cells showed that the amine content in these vesicles is reduced by half. These data demonstrate that although chromogranins regulate the amine concentration in LDCVs, they are not completely essential, and other proteins unrelated to neurosecretion, such as fibrinogen, might compensate for their loss to ensure that vesicles are generated and the secretory pathway conserved.—Díaz‐Vera, J., Camacho, M., Machado, J. D., Domínguez, N., Montesinos, M. S., Hernández‐Fernaud, J. R., Luján, R., Borges, R. Chromogranins A and B are key proteins in amine accumulation, but the catecholamine secretory pathway is conserved without them. FASEB J. 26, 430–438 (2012). www.fasebj.org

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Crossref · 10.1096/fj.11-181941

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Authors
Díaz‐Vera, J., Camacho, M., Machado, J. D., Domínguez, N., Montesinos, M. S., Hernández‐Fernaud, J. R., Luján, R., & Borges, R.
Affiliations
  • Unidad de Farmacología, Facultad de Medicina Universidad de La Laguna Tenerife Spain
  • Universidad de La Laguna (ES)
  • University of Castilla-La Mancha (ES)
Corresponding author
Borges, Ricardo
Venue
The FASEB Journal
Publisher
Wiley
Published
2011-10-11
Volume
26
Issue
1
Pages
430-438
ISSN
  • 0892-6638 (ISSN-L)
  • 1530-6860
OpenAlex
W2099298311
Cited by
55
References
36
Citations by year
2026: 3 · 2025: 1 · 2023: 6 · 2022: 2 · 2021: 1 · 2020: 2 · 2019: 7 · 2018: 3 · 2017: 9 · 2016: 4 · 2015: 2 · 2014: 4 · 2013: 5 · 2012: 6
FWCI
2.30
Type
journal article
Language
en
Open access
closed
Locations
  • The FASEB Journal (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular transport and secretion
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Extracellular vesicles in disease
Keywords
chromogranin A, chromogranin B, chromaffin cells, catecholamine release, epinephrine, amine accumulation, vesicle biogenesis, fibrinogen, neurosecretion
MeSH terms
Adrenal Medulla, Animals, Catecholamines, Cells, Cultured, Cytosol, Female, Levodopa, Male, Membrane Potentials, Phenotype, Pregnancy, Tyrosine 3-Monooxygenase, Dopamine Agents, Mice, Knockout, Chromaffin Cells, Mice, Chromogranin B, Chromogranin A, Secretory Pathway

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