Chromogranins A and B are key proteins in amine accumulation, but the catecholamine secretory pathway is conserved without them
Abstract
ABSTRACT Chromogranins are the main soluble proteins in the large dense core secretory vesicles (LDCVs) found in aminergic neurons and chromaffin cells. We recently demonstrated that chromogranins A and B each regulate the concentration of adrenaline in chromaffin granules and its exocytosis. Here we have further studied the role played by these proteins by generating mice lacking both chromogranins. Surprisingly, these animals are both viable and fertile. Although chromogranins are thought to be essential for their biogenesis, LDCVs were evident in these mice. These vesicles do have a somewhat atypical appearance and larger size. Despite their increased size, single‐cell amperometry recordings from chromaffin cells showed that the amine content in these vesicles is reduced by half. These data demonstrate that although chromogranins regulate the amine concentration in LDCVs, they are not completely essential, and other proteins unrelated to neurosecretion, such as fibrinogen, might compensate for their loss to ensure that vesicles are generated and the secretory pathway conserved.—Díaz‐Vera, J., Camacho, M., Machado, J. D., Domínguez, N., Montesinos, M. S., Hernández‐Fernaud, J. R., Luján, R., Borges, R. Chromogranins A and B are key proteins in amine accumulation, but the catecholamine secretory pathway is conserved without them. FASEB J. 26, 430–438 (2012). www.fasebj.org
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Chga regulates the biogenesis of catecholamine-containing DCGs in the adrenal gland.
“Chga has been shown to regulate the biogenesis of catecholamine-containing DCGs in the adrenal gland (Díaz-Vera et al., 2012; Kim et al., 2001; Pasqua et al., 2016).”
From Intestinal microbiota modulates adrenomedullary response through Nod1 sensing in chromaffin cells (2021) · cites this paper as Díaz-Vera et al., 2012
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- Authors
- Díaz‐Vera, J., Camacho, M., Machado, J. D., Domínguez, N., Montesinos, M. S., Hernández‐Fernaud, J. R., Luján, R., & Borges, R.
- Affiliations
- Unidad de Farmacología, Facultad de Medicina Universidad de La Laguna Tenerife Spain
- Universidad de La Laguna (ES)
- University of Castilla-La Mancha (ES)
- Corresponding author
- Borges, Ricardo
- Venue
- The FASEB Journal
- Publisher
- Wiley
- Published
- 2011-10-11
- Volume
- 26
- Issue
- 1
- Pages
- 430-438
- ISSN
- 0892-6638 (ISSN-L)
- 1530-6860
- OpenAlex
- W2099298311
- Cited by
- 55
- References
- 36
- Citations by year
- 2026: 3 · 2025: 1 · 2023: 6 · 2022: 2 · 2021: 1 · 2020: 2 · 2019: 7 · 2018: 3 · 2017: 9 · 2016: 4 · 2015: 2 · 2014: 4 · 2013: 5 · 2012: 6
- FWCI
- 2.30
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- The FASEB Journal (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular transport and secretion
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Extracellular vesicles in disease
- Keywords
- chromogranin A, chromogranin B, chromaffin cells, catecholamine release, epinephrine, amine accumulation, vesicle biogenesis, fibrinogen, neurosecretion
- MeSH terms
- Adrenal Medulla, Animals, Catecholamines, Cells, Cultured, Cytosol, Female, Levodopa, Male, Membrane Potentials, Phenotype, Pregnancy, Tyrosine 3-Monooxygenase, Dopamine Agents, Mice, Knockout, Chromaffin Cells, Mice, Chromogranin B, Chromogranin A, Secretory Pathway
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